A baicalin-loaded coaxial nanofiber scaffold regulated inflammation and osteoclast differentiation for vascularized bone regeneration

• Main Authors: Shue Jin, Jing Gao, Renli Yang, Chen Yuan, Ruili Wang, Qin Zou, Yi Zuo, Meifang Zhu, Yubao Li, Yi Man, Jidong Li
• Format: Article
• Language: English
• Published: KeAi Communications Co., Ltd. 2022-02-01
• Series: Bioactive Materials
• Subjects: Coaxial nanofiber; Baicalin; Inflammation; Osteoclast differentiation; Vascularized bone regeneration
• Online Access: http://www.sciencedirect.com/science/article/pii/S2452199X21003145

We demonstrate a simple, effective and feasible method to address the shrinkage of Poly (lactic-co-glycolic acid) (PLGA) through a core-shell structure fiber strategy. The results revealed that introducing size-stable poly-caprolactone (PCL) as the core fiber significantly improved the PLGA-based fibrous scaffold's dimensional maintenance. We further utilized fish collagen to modify the PLGA shell layer (PFC) of coaxial fibers and loaded baicalin (BA) into the PCL core layer (PCL-BA) to endow fibrous scaffold with more functional biological cues. The PFC/PCL-BA fibrous scaffold promoted the osteogenic differentiation of bone mesenchymal stem cells and stimulated the RAW264.7 cells to polarize into a pro-reparative phenotype. Importantly, the in vivo study demonstrated that the PFC/PCL-BA scaffold could regulate inflammation and osteoclast differentiation, favor neovascularization and bone formation. This work tactfully combined PLGA and PCL to establish a drug release platform based on the core-shell fibrous scaffold for vascularized bone regeneration.