2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals
Abstract ᅟ Previous reports suggest that the 2-methyl butyramide and 2-methyl valeramide metabolites of Ascaris lumbricoides in urine of infected individuals could be considered as urinary biomarkers for active infection. We have developed an LC-MS method with a detection limit of 10 ng/mL using syn...
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doaj-cb9abf0a37b0487cbbce6f8fb75985a12020-11-24T21:12:08ZengBMCParasites & Vectors1756-33052017-12-011011310.1186/s13071-017-2600-z2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individualsOle Lagatie0Emmanuel Njumbe Ediage1Jeroen A. Pikkemaat2Yenny Djuardi3Lieven J. Stuyver4Janssen Diagnostics, Janssen R&DBioanalysis, Janssen R&DPharmaceutical Development and Manufacturing Sciences, Janssen R&DDepartment of Parasitology, Faculty of Medicine, Universitas IndonesiaJanssen Diagnostics, Janssen R&DAbstract ᅟ Previous reports suggest that the 2-methyl butyramide and 2-methyl valeramide metabolites of Ascaris lumbricoides in urine of infected individuals could be considered as urinary biomarkers for active infection. We have developed an LC-MS method with a detection limit of 10 ng/mL using synthetic chemicals as reference material. Urine samples (n = 21) of infected individuals were analyzed for the presence of these metabolites, but they were not detected in any of the samples. Furthermore, the recorded 1H-NMR spectrum for reference 2-methyl butyramide did not match with the spectrum that was described for the Ascaris metabolite. Based on these two observations, we concluded that the urinary biomarkers that were detected for A. lumbricoides infection are not 2-methyl butyramide nor 2-methylvaleramide. New discovery efforts will be required to identify the structure of these metabolite biomarkers in urine of infected individuals. Trial registration Urine samples used in this study were collected as part of a clinical trial with trial number ISRCTN75636394 (12 November 2013).http://link.springer.com/article/10.1186/s13071-017-2600-zAscaris lumbricoidesSoil-transmitted helminthsBiomarkerUrine2-methyl butyramide2-methyl valeramide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ole Lagatie Emmanuel Njumbe Ediage Jeroen A. Pikkemaat Yenny Djuardi Lieven J. Stuyver |
spellingShingle |
Ole Lagatie Emmanuel Njumbe Ediage Jeroen A. Pikkemaat Yenny Djuardi Lieven J. Stuyver 2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals Parasites & Vectors Ascaris lumbricoides Soil-transmitted helminths Biomarker Urine 2-methyl butyramide 2-methyl valeramide |
author_facet |
Ole Lagatie Emmanuel Njumbe Ediage Jeroen A. Pikkemaat Yenny Djuardi Lieven J. Stuyver |
author_sort |
Ole Lagatie |
title |
2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals |
title_short |
2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals |
title_full |
2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals |
title_fullStr |
2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals |
title_full_unstemmed |
2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals |
title_sort |
2-methyl butyramide, a previously identified urine biomarker for ascaris lumbricoides, is not present in infected indonesian individuals |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2017-12-01 |
description |
Abstract ᅟ Previous reports suggest that the 2-methyl butyramide and 2-methyl valeramide metabolites of Ascaris lumbricoides in urine of infected individuals could be considered as urinary biomarkers for active infection. We have developed an LC-MS method with a detection limit of 10 ng/mL using synthetic chemicals as reference material. Urine samples (n = 21) of infected individuals were analyzed for the presence of these metabolites, but they were not detected in any of the samples. Furthermore, the recorded 1H-NMR spectrum for reference 2-methyl butyramide did not match with the spectrum that was described for the Ascaris metabolite. Based on these two observations, we concluded that the urinary biomarkers that were detected for A. lumbricoides infection are not 2-methyl butyramide nor 2-methylvaleramide. New discovery efforts will be required to identify the structure of these metabolite biomarkers in urine of infected individuals. Trial registration Urine samples used in this study were collected as part of a clinical trial with trial number ISRCTN75636394 (12 November 2013). |
topic |
Ascaris lumbricoides Soil-transmitted helminths Biomarker Urine 2-methyl butyramide 2-methyl valeramide |
url |
http://link.springer.com/article/10.1186/s13071-017-2600-z |
work_keys_str_mv |
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