Quality control methods for Aedes albopictus sterile male production.
The capacity of the released sterile males to survive, disperse, compete with wild males and inseminate wild females is an essential prerequisite to be evaluated in any area-wide integrated pest management (AW-IPM) programs including a sterile insect release method. Adequate quality control tests su...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017-09-01
|
Series: | PLoS Neglected Tropical Diseases |
Online Access: | http://europepmc.org/articles/PMC5608434?pdf=render |
id |
doaj-cba05aa2126048569af792be4ac72ad1 |
---|---|
record_format |
Article |
spelling |
doaj-cba05aa2126048569af792be4ac72ad12020-11-25T02:47:00ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352017-09-01119e000588110.1371/journal.pntd.0005881Quality control methods for Aedes albopictus sterile male production.Fabrizio BalestrinoArianna PuggioliMarco CarrieriJérémy BouyerRomeo BelliniThe capacity of the released sterile males to survive, disperse, compete with wild males and inseminate wild females is an essential prerequisite to be evaluated in any area-wide integrated pest management (AW-IPM) programs including a sterile insect release method. Adequate quality control tests supported by standardized procedures need to be developed to measure these parameters and to identify and correct potential inappropriate rearing or handling methods affecting the overall male quality. In this study, we report results on the creation and validation of the first quality control devices designed to infer the survival and mating capacity of radio-sterilized Aedes albopictus males through the observation of their flight capacity under restricted conditions (flight organ device) and after stress treatment (aspirator device). Results obtained consistently indicate comparable flight capacity and quality parameters between untreated and 35 Gy irradiated males while a negative impact was observed with higher radiation doses at all observation time performed. The male flight capacity registered with the proposed quality control devices can be successfully employed, with different predictive capacities and response time, to infer the adult male quality. These simple and cost-effective tools provide a valuable method to detect and amend potentially sub-standard procedures in the sterile male production line and hence contribute to maintaining optimal quality and field performance of the mosquitoes being released.http://europepmc.org/articles/PMC5608434?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fabrizio Balestrino Arianna Puggioli Marco Carrieri Jérémy Bouyer Romeo Bellini |
spellingShingle |
Fabrizio Balestrino Arianna Puggioli Marco Carrieri Jérémy Bouyer Romeo Bellini Quality control methods for Aedes albopictus sterile male production. PLoS Neglected Tropical Diseases |
author_facet |
Fabrizio Balestrino Arianna Puggioli Marco Carrieri Jérémy Bouyer Romeo Bellini |
author_sort |
Fabrizio Balestrino |
title |
Quality control methods for Aedes albopictus sterile male production. |
title_short |
Quality control methods for Aedes albopictus sterile male production. |
title_full |
Quality control methods for Aedes albopictus sterile male production. |
title_fullStr |
Quality control methods for Aedes albopictus sterile male production. |
title_full_unstemmed |
Quality control methods for Aedes albopictus sterile male production. |
title_sort |
quality control methods for aedes albopictus sterile male production. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Neglected Tropical Diseases |
issn |
1935-2727 1935-2735 |
publishDate |
2017-09-01 |
description |
The capacity of the released sterile males to survive, disperse, compete with wild males and inseminate wild females is an essential prerequisite to be evaluated in any area-wide integrated pest management (AW-IPM) programs including a sterile insect release method. Adequate quality control tests supported by standardized procedures need to be developed to measure these parameters and to identify and correct potential inappropriate rearing or handling methods affecting the overall male quality. In this study, we report results on the creation and validation of the first quality control devices designed to infer the survival and mating capacity of radio-sterilized Aedes albopictus males through the observation of their flight capacity under restricted conditions (flight organ device) and after stress treatment (aspirator device). Results obtained consistently indicate comparable flight capacity and quality parameters between untreated and 35 Gy irradiated males while a negative impact was observed with higher radiation doses at all observation time performed. The male flight capacity registered with the proposed quality control devices can be successfully employed, with different predictive capacities and response time, to infer the adult male quality. These simple and cost-effective tools provide a valuable method to detect and amend potentially sub-standard procedures in the sterile male production line and hence contribute to maintaining optimal quality and field performance of the mosquitoes being released. |
url |
http://europepmc.org/articles/PMC5608434?pdf=render |
work_keys_str_mv |
AT fabriziobalestrino qualitycontrolmethodsforaedesalbopictussterilemaleproduction AT ariannapuggioli qualitycontrolmethodsforaedesalbopictussterilemaleproduction AT marcocarrieri qualitycontrolmethodsforaedesalbopictussterilemaleproduction AT jeremybouyer qualitycontrolmethodsforaedesalbopictussterilemaleproduction AT romeobellini qualitycontrolmethodsforaedesalbopictussterilemaleproduction |
_version_ |
1724755324624175104 |