A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression
Abstract Background Obesity is a global epidemic disease that increases the risk of metabolic syndrome. However, therapeutic drugs for obesity are still scarce. In recent years, peptides have been identified as new biological regulators. RIFV (R-I-F-V-P-I-K-G-R-P-A-P), a novel active peptide from ou...
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doaj-cbace0aac266413f865766ff999eaf522020-12-27T12:19:43ZengBMCNutrition & Metabolism1743-70752019-12-0116111010.1186/s12986-019-0414-zA novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expressionWen Zhang0Dan Shen1Yun Li2Hong Zhong3Xing Wang4Xian-wei Cui5Chun-Mei Shi6Chen-Bo Ji7Xi-Rong Guo8Ling Chen9Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityDepartment of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityDepartment of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityDepartment of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityDepartment of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityDepartment of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityDepartment of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityDepartment of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityHongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical UniversityAbstract Background Obesity is a global epidemic disease that increases the risk of metabolic syndrome. However, therapeutic drugs for obesity are still scarce. In recent years, peptides have been identified as new biological regulators. RIFV (R-I-F-V-P-I-K-G-R-P-A-P), a novel active peptide from our peptide database. Methods We performed oil red O staining and triglyceride measurement to analyze the influence of RIFV on white preadipocytes differentiation. Then the effects of RIFV on cell proliferation, apoptosis and cell cycle were determined by using CCK-8 assay and flow cytometry. The mRNA and protein levels of adipogenesis-related genes were respectively detected by qRT-PCR and western blot. Rescue experiment was conducted to confirm whether RIFV could regulate adipocytes differentiation via targeting C/EBP-β. Finally, the luciferase reporter gene assay was performed to verify the regulation of RIFV on C/EBP-β gene. Results RIFV was revealed to inhibit the differentiation of human white adipocytes without affecting their proliferation. Additionally, RIFV could also suppress the differentiation of mouse primary white preadipocytes isolated from inguinal fat tissues. Furthermore, RIFV may have an inhibitory effect on adipogenesis by inhibiting the regulation of the adipogenic gene C/EBP-β. Conclusions Our results indicated that RIFV may be a novel essential regulator of adipocyte differentiation and represents a therapeutic strategy for obesity and related complications.https://doi.org/10.1186/s12986-019-0414-zRIFVPeptidesAdipocyte differentiationC/EBP-β |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen Zhang Dan Shen Yun Li Hong Zhong Xing Wang Xian-wei Cui Chun-Mei Shi Chen-Bo Ji Xi-Rong Guo Ling Chen |
spellingShingle |
Wen Zhang Dan Shen Yun Li Hong Zhong Xing Wang Xian-wei Cui Chun-Mei Shi Chen-Bo Ji Xi-Rong Guo Ling Chen A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression Nutrition & Metabolism RIFV Peptides Adipocyte differentiation C/EBP-β |
author_facet |
Wen Zhang Dan Shen Yun Li Hong Zhong Xing Wang Xian-wei Cui Chun-Mei Shi Chen-Bo Ji Xi-Rong Guo Ling Chen |
author_sort |
Wen Zhang |
title |
A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression |
title_short |
A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression |
title_full |
A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression |
title_fullStr |
A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression |
title_full_unstemmed |
A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression |
title_sort |
novel peptide rifv suppresses human adipocyte differentiation through the inhibition of c/ebp-β expression |
publisher |
BMC |
series |
Nutrition & Metabolism |
issn |
1743-7075 |
publishDate |
2019-12-01 |
description |
Abstract Background Obesity is a global epidemic disease that increases the risk of metabolic syndrome. However, therapeutic drugs for obesity are still scarce. In recent years, peptides have been identified as new biological regulators. RIFV (R-I-F-V-P-I-K-G-R-P-A-P), a novel active peptide from our peptide database. Methods We performed oil red O staining and triglyceride measurement to analyze the influence of RIFV on white preadipocytes differentiation. Then the effects of RIFV on cell proliferation, apoptosis and cell cycle were determined by using CCK-8 assay and flow cytometry. The mRNA and protein levels of adipogenesis-related genes were respectively detected by qRT-PCR and western blot. Rescue experiment was conducted to confirm whether RIFV could regulate adipocytes differentiation via targeting C/EBP-β. Finally, the luciferase reporter gene assay was performed to verify the regulation of RIFV on C/EBP-β gene. Results RIFV was revealed to inhibit the differentiation of human white adipocytes without affecting their proliferation. Additionally, RIFV could also suppress the differentiation of mouse primary white preadipocytes isolated from inguinal fat tissues. Furthermore, RIFV may have an inhibitory effect on adipogenesis by inhibiting the regulation of the adipogenic gene C/EBP-β. Conclusions Our results indicated that RIFV may be a novel essential regulator of adipocyte differentiation and represents a therapeutic strategy for obesity and related complications. |
topic |
RIFV Peptides Adipocyte differentiation C/EBP-β |
url |
https://doi.org/10.1186/s12986-019-0414-z |
work_keys_str_mv |
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