An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case–case–control study
Abstract Background Asthma and allergic rhinitis are respiratory diseases with a significant global burden. Forkhead box O3 (FOXO3) is a gene involved in the etiology of a number of respiratory diseases. The objective of this study is to assess the association of rs13217795, an intronic FOXO3 single...
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doaj-cbb0b7e8746d4ade854ddd74dc3e1b262021-04-02T08:24:01ZengBMCBMC Medical Genetics1471-23502017-11-011811610.1186/s12881-017-0494-4An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case–case–control studyJustin Z. Amarin0Randa G. Naffa1Haya H. Suradi2Yousof M. Alsaket3Nathir M. Obeidat4Tareq M. Mahafza5Malek A. Zihlif6School of Medicine, The University of JordanMolecular Biology Research Laboratory, School of Medicine, The University of JordanSchool of Medicine, The University of JordanSchool of Dentistry, The University of JordanDepartment of Internal Medicine, School of Medicine, The University of JordanDepartment of Special Surgery, School of Medicine, The University of JordanDepartment of Pharmacology, School of Medicine, The University of JordanAbstract Background Asthma and allergic rhinitis are respiratory diseases with a significant global burden. Forkhead box O3 (FOXO3) is a gene involved in the etiology of a number of respiratory diseases. The objective of this study is to assess the association of rs13217795, an intronic FOXO3 single-nucleotide polymorphism, with asthma and allergic rhinitis. Methods In this case–case–control genetic association study, genotyping was conducted using the PCR–RFLP method. Genotype-based associations were investigated under the general, recessive, and dominant models of disease penetrance using binomial logistic regression; and, allele-based associations were tested using Pearson’s chi-squared test. Results The final study population consisted of 94 controls, 124 asthmatics, and 110 allergic rhinitis patients. The general and recessive models of disease penetrance were statistically significant for both case–control comparisons. Under the general model, the odds of the asthma phenotype were 1.46 (0.64 to 3.34) and 3.42 (1.37 to 8.57) times higher in heterozygotes and derived allele homozygotes, respectively, compared to ancestral allele homozygotes. The corresponding odds ratios for the allergic rhinitis phenotype were 1.05 (0.46 to 2.40) and 2.35 (0.96 to 5.73), respectively. The dominant model of disease penetrance was not statistically significant. The minor allele in all study groups was the ancestral allele, with a frequency of 0.49 in controls. There was no deviation from Hardy–Weinberg equilibrium in controls. Both case–control allele-based associations were statistically significant. Conclusions Herein we present the first report of the association between rs13217795 and allergic rhinitis, and the first independent verification of the association between rs13217795 and asthma. Marker selection in future genetic association studies of asthma and allergic rhinitis should include functional polymorphisms in linkage disequilibrium with rs13217795.http://link.springer.com/article/10.1186/s12881-017-0494-4FOXO3AsthmaAllergic rhinitisSingle-nucleotide polymorphismrs13217795 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Justin Z. Amarin Randa G. Naffa Haya H. Suradi Yousof M. Alsaket Nathir M. Obeidat Tareq M. Mahafza Malek A. Zihlif |
spellingShingle |
Justin Z. Amarin Randa G. Naffa Haya H. Suradi Yousof M. Alsaket Nathir M. Obeidat Tareq M. Mahafza Malek A. Zihlif An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case–case–control study BMC Medical Genetics FOXO3 Asthma Allergic rhinitis Single-nucleotide polymorphism rs13217795 |
author_facet |
Justin Z. Amarin Randa G. Naffa Haya H. Suradi Yousof M. Alsaket Nathir M. Obeidat Tareq M. Mahafza Malek A. Zihlif |
author_sort |
Justin Z. Amarin |
title |
An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case–case–control study |
title_short |
An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case–case–control study |
title_full |
An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case–case–control study |
title_fullStr |
An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case–case–control study |
title_full_unstemmed |
An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case–case–control study |
title_sort |
intronic single-nucleotide polymorphism (rs13217795) in foxo3 is associated with asthma and allergic rhinitis: a case–case–control study |
publisher |
BMC |
series |
BMC Medical Genetics |
issn |
1471-2350 |
publishDate |
2017-11-01 |
description |
Abstract Background Asthma and allergic rhinitis are respiratory diseases with a significant global burden. Forkhead box O3 (FOXO3) is a gene involved in the etiology of a number of respiratory diseases. The objective of this study is to assess the association of rs13217795, an intronic FOXO3 single-nucleotide polymorphism, with asthma and allergic rhinitis. Methods In this case–case–control genetic association study, genotyping was conducted using the PCR–RFLP method. Genotype-based associations were investigated under the general, recessive, and dominant models of disease penetrance using binomial logistic regression; and, allele-based associations were tested using Pearson’s chi-squared test. Results The final study population consisted of 94 controls, 124 asthmatics, and 110 allergic rhinitis patients. The general and recessive models of disease penetrance were statistically significant for both case–control comparisons. Under the general model, the odds of the asthma phenotype were 1.46 (0.64 to 3.34) and 3.42 (1.37 to 8.57) times higher in heterozygotes and derived allele homozygotes, respectively, compared to ancestral allele homozygotes. The corresponding odds ratios for the allergic rhinitis phenotype were 1.05 (0.46 to 2.40) and 2.35 (0.96 to 5.73), respectively. The dominant model of disease penetrance was not statistically significant. The minor allele in all study groups was the ancestral allele, with a frequency of 0.49 in controls. There was no deviation from Hardy–Weinberg equilibrium in controls. Both case–control allele-based associations were statistically significant. Conclusions Herein we present the first report of the association between rs13217795 and allergic rhinitis, and the first independent verification of the association between rs13217795 and asthma. Marker selection in future genetic association studies of asthma and allergic rhinitis should include functional polymorphisms in linkage disequilibrium with rs13217795. |
topic |
FOXO3 Asthma Allergic rhinitis Single-nucleotide polymorphism rs13217795 |
url |
http://link.springer.com/article/10.1186/s12881-017-0494-4 |
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