Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma.
High hydrostatic pressure has been used to inactivate pathogens in foods for decades. There is a great potential to adapt this technology to inactivate pathogens in plasma and derivatives. To better evaluate the potential of this method, pathogen inoculated plasma samples were pressurized under diff...
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2016-01-01
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doaj-cbb231874fd748b0a902ea98e0e611632020-11-25T01:30:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01118e016177510.1371/journal.pone.0161775Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma.Chunhui YangGuohui BianHong YangXinmin ZhangLimin ChenJingxing WangHigh hydrostatic pressure has been used to inactivate pathogens in foods for decades. There is a great potential to adapt this technology to inactivate pathogens in plasma and derivatives. To better evaluate the potential of this method, pathogen inoculated plasma samples were pressurized under different pressure application modes and temperatures. The inactivation efficacy of pathogens and activities of plasma proteins were monitored after treatment. The CFUs of E.coli was examined as the indicator of the inactivation efficiency. The factor V and VIII were chosen as the indicator of the plasma function. Preliminary experiments identified optimized treatment conditions: 200-250MPa, with 5×1 minute multi-pulsed high pressure at near 0°C (ice-water bath). Under this conditions, the inactivation efficacy of EMCV was >8.5log. The CFUs of E. coli were reduced by 7.5log, B. cereus were 8log. However, PPV and S. aureus cannot be inactivated efficiently. The activities of factor II, VII, IX, X, XI, XII, fibrinogen, IgG, IgM stayed over 95% compared to untreated. Factor V and VIII activity was maintained at 46-63% and 77-82%, respectively.http://europepmc.org/articles/PMC4999174?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chunhui Yang Guohui Bian Hong Yang Xinmin Zhang Limin Chen Jingxing Wang |
spellingShingle |
Chunhui Yang Guohui Bian Hong Yang Xinmin Zhang Limin Chen Jingxing Wang Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma. PLoS ONE |
author_facet |
Chunhui Yang Guohui Bian Hong Yang Xinmin Zhang Limin Chen Jingxing Wang |
author_sort |
Chunhui Yang |
title |
Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma. |
title_short |
Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma. |
title_full |
Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma. |
title_fullStr |
Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma. |
title_full_unstemmed |
Development of High Hydrostatic Pressure Applied in Pathogen Inactivation for Plasma. |
title_sort |
development of high hydrostatic pressure applied in pathogen inactivation for plasma. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
High hydrostatic pressure has been used to inactivate pathogens in foods for decades. There is a great potential to adapt this technology to inactivate pathogens in plasma and derivatives. To better evaluate the potential of this method, pathogen inoculated plasma samples were pressurized under different pressure application modes and temperatures. The inactivation efficacy of pathogens and activities of plasma proteins were monitored after treatment. The CFUs of E.coli was examined as the indicator of the inactivation efficiency. The factor V and VIII were chosen as the indicator of the plasma function. Preliminary experiments identified optimized treatment conditions: 200-250MPa, with 5×1 minute multi-pulsed high pressure at near 0°C (ice-water bath). Under this conditions, the inactivation efficacy of EMCV was >8.5log. The CFUs of E. coli were reduced by 7.5log, B. cereus were 8log. However, PPV and S. aureus cannot be inactivated efficiently. The activities of factor II, VII, IX, X, XI, XII, fibrinogen, IgG, IgM stayed over 95% compared to untreated. Factor V and VIII activity was maintained at 46-63% and 77-82%, respectively. |
url |
http://europepmc.org/articles/PMC4999174?pdf=render |
work_keys_str_mv |
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