Immune transcriptome alterations in the temporal cortex of subjects with autism

Immune transcriptome alterations in the temporal cortex of subjects with autism

Autism is a severe disorder that involves both genetic and environmental factors. Expression profiling of the superior temporal gyrus of six autistic subjects and matched controls revealed increased transcript levels of many immune system-related genes. We also noticed changes in transcripts related...

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Main Authors: Krassimira Garbett, Philip J. Ebert, Amanda Mitchell, Carla Lintas, Barbara Manzi, Károly Mirnics, Antonio M. Persico
Format: Article
Language:English
Published: Elsevier 2008-06-01
Series:Neurobiology of Disease
Subjects:
DNA microarray
Gene expression
Transcriptome
Autism
Post mortem
Temporal cortex
Online Access:http://www.sciencedirect.com/science/article/pii/S0969996108000247
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spelling doaj-cbc6c6028903476494c27365984ba3652021-03-20T04:55:32ZengElsevierNeurobiology of Disease1095-953X2008-06-01303303311Immune transcriptome alterations in the temporal cortex of subjects with autismKrassimira Garbett0Philip J. Ebert1Amanda Mitchell2Carla Lintas3Barbara Manzi4Károly Mirnics5Antonio M. Persico6Department of Psychiatry, Vanderbilt University, Nashville, USADepartment of Psychiatry, Vanderbilt University, Nashville, USADepartment of Psychiatry, Vanderbilt University, Nashville, USALaboratory of Molecular Psychiatry and Neurogenetics, University “Campus Bio-Medico”, Rome, Italy; Laboratory of Molecular Psychiatry and Psychiatric Genetics, Department of Experimental Neurosciences, I.R.C.C.S. “Fondazione Santa Lucia”, Rome, ItalyDepartment of Child Neuropsychiatry, University “Tor Vergata”, Rome, ItalyDepartment of Psychiatry, Vanderbilt University, Nashville, USA; Vanderbilt Kennedy Center for Research on Human Development, Vanderbilt University, Nashville, USA; Corresponding authors. K. Mirnics is to be contacted at Department of Psychiatry, Vanderbilt University, 8130A MRB III, 465 21st Avenue South, Nashville TN 37203, USA. A.M. Persico, Laboratory of Molecular Psychiatry and Neurogenetics, University “Campus Bio-Medico”, Via Alvaro del Portillo 21, I-00128 Rome, ItalyLaboratory of Molecular Psychiatry and Neurogenetics, University “Campus Bio-Medico”, Rome, Italy; Laboratory of Molecular Psychiatry and Psychiatric Genetics, Department of Experimental Neurosciences, I.R.C.C.S. “Fondazione Santa Lucia”, Rome, Italy; Corresponding authors. K. Mirnics is to be contacted at Department of Psychiatry, Vanderbilt University, 8130A MRB III, 465 21st Avenue South, Nashville TN 37203, USA. A.M. Persico, Laboratory of Molecular Psychiatry and Neurogenetics, University “Campus Bio-Medico”, Via Alvaro del Portillo 21, I-00128 Rome, ItalyAutism is a severe disorder that involves both genetic and environmental factors. Expression profiling of the superior temporal gyrus of six autistic subjects and matched controls revealed increased transcript levels of many immune system-related genes. We also noticed changes in transcripts related to cell communication, differentiation, cell cycle regulation and chaperone systems. Critical expression changes were confirmed by qPCR (BCL6, CHI3L1, CYR61, IFI16, IFITM3, MAP2K3, PTDSR, RFX4, SPP1, RELN, NOTCH2, RIT1, SFN, GADD45B, HSPA6, HSPB8 and SERPINH1). Overall, these expression patterns appear to be more associated with the late recovery phase of autoimmune brain disorders, than with the innate immune response characteristic of neurodegenerative diseases. Moreover, a variance-based analysis revealed much greater transcript variability in brains from autistic subjects compared to the control group, suggesting that these genes may represent autism susceptibility genes and should be assessed in follow-up genetic studies.http://www.sciencedirect.com/science/article/pii/S0969996108000247DNA microarrayGene expressionTranscriptomeAutismPost mortemTemporal cortex
collection DOAJ
language English
format Article
sources DOAJ
author Krassimira Garbett
Philip J. Ebert
Amanda Mitchell
Carla Lintas
Barbara Manzi
Károly Mirnics
Antonio M. Persico
spellingShingle Krassimira Garbett
Philip J. Ebert
Amanda Mitchell
Carla Lintas
Barbara Manzi
Károly Mirnics
Antonio M. Persico
Immune transcriptome alterations in the temporal cortex of subjects with autism
Neurobiology of Disease
DNA microarray
Gene expression
Transcriptome
Autism
Post mortem
Temporal cortex
author_facet Krassimira Garbett
Philip J. Ebert
Amanda Mitchell
Carla Lintas
Barbara Manzi
Károly Mirnics
Antonio M. Persico
author_sort Krassimira Garbett
title Immune transcriptome alterations in the temporal cortex of subjects with autism
title_short Immune transcriptome alterations in the temporal cortex of subjects with autism
title_full Immune transcriptome alterations in the temporal cortex of subjects with autism
title_fullStr Immune transcriptome alterations in the temporal cortex of subjects with autism
title_full_unstemmed Immune transcriptome alterations in the temporal cortex of subjects with autism
title_sort immune transcriptome alterations in the temporal cortex of subjects with autism
publisher Elsevier
series Neurobiology of Disease
issn 1095-953X
publishDate 2008-06-01
description Autism is a severe disorder that involves both genetic and environmental factors. Expression profiling of the superior temporal gyrus of six autistic subjects and matched controls revealed increased transcript levels of many immune system-related genes. We also noticed changes in transcripts related to cell communication, differentiation, cell cycle regulation and chaperone systems. Critical expression changes were confirmed by qPCR (BCL6, CHI3L1, CYR61, IFI16, IFITM3, MAP2K3, PTDSR, RFX4, SPP1, RELN, NOTCH2, RIT1, SFN, GADD45B, HSPA6, HSPB8 and SERPINH1). Overall, these expression patterns appear to be more associated with the late recovery phase of autoimmune brain disorders, than with the innate immune response characteristic of neurodegenerative diseases. Moreover, a variance-based analysis revealed much greater transcript variability in brains from autistic subjects compared to the control group, suggesting that these genes may represent autism susceptibility genes and should be assessed in follow-up genetic studies.
topic DNA microarray
Gene expression
Transcriptome
Autism
Post mortem
Temporal cortex
url http://www.sciencedirect.com/science/article/pii/S0969996108000247
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