Pulse-shaping-based two-photon FRET microscopy
We present quantitative pulse-shaping-based two-photon fluorescence resonance energy transfer microscopy. We tailor the spectral phase of the excitation pulses to achieve selective excitation of donor and acceptor, demonstrating the method in live cells.
Main Authors: | Swanson J. A., Straight S. W., Cai D., Brenner M. H., Ogilvie J. P. |
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Format: | Article |
Language: | English |
Published: |
EDP Sciences
2013-03-01
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Series: | EPJ Web of Conferences |
Online Access: | http://dx.doi.org/10.1051/epjconf/20134111009 |
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