Advanced Extraction of Lipids with DHA from <i>Isochrysis galbana</i> with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions
Microalgal biomass is a sustainable and valuable source of lipids with omega-3 fatty acids. The efficient extraction of lipids from microalgae requires fast and alternative extraction methods, frequently combined with biomass pre-treatment by different procedures. In this work, Pressurized liquid ex...
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doaj-cbcc886f42f347d69dab9e0430ae587a2020-11-25T03:11:22ZengMDPI AGMolecules1420-30492020-07-01253310331010.3390/molecules25143310Advanced Extraction of Lipids with DHA from <i>Isochrysis galbana</i> with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted ExtractionsMaría Señoráns0Natalia Castejón1Francisco Javier Señoráns2Healthy-Lipids Group, Sección Departamental de Ciencias de la Alimentación, Faculty of Sciences, Universidad Autónoma de Madrid, 28049 Madrid, SpainHealthy-Lipids Group, Sección Departamental de Ciencias de la Alimentación, Faculty of Sciences, Universidad Autónoma de Madrid, 28049 Madrid, SpainHealthy-Lipids Group, Sección Departamental de Ciencias de la Alimentación, Faculty of Sciences, Universidad Autónoma de Madrid, 28049 Madrid, SpainMicroalgal biomass is a sustainable and valuable source of lipids with omega-3 fatty acids. The efficient extraction of lipids from microalgae requires fast and alternative extraction methods, frequently combined with biomass pre-treatment by different procedures. In this work, Pressurized liquid extraction (PLE) was optimized and compared with traditional lipid extraction methods, Folch and Bligh and Dyer, and with a new Ultrasound Assisted Extraction (UAE) method for lipids from microalgae <i>Isochrysis galbana</i>. To further optimize PLE and UAE, enzymatic pre-treatment of microalga <i>Isochrysis galbana</i> was studied with commercial enzymes Viscozyme and Celluclast. No significant differences were found for lipid yields among different extraction techniques used. However, advanced extraction techniques with or without pre-treatment are a green, fast, and toxic solvent free alternative to traditional techniques. Lipid composition of <i>Isochrysis</i> was determined by HPLC-ELSD and included neutral and polar lipids, showing that each fraction comprised different contents in omega-3 polyunsaturated fatty acids (PUFA). The highest polar lipids content was achieved with UAE (50 °C and 15 min) and PLE (100 °C) techniques. Moreover, the highest omega-3 PUFA (33.2%), eicosapentaenoic acid (EPA) (3.3%) and docosahexaenoic acid (DHA) (12.0%) contents were achieved with the advanced technique UAE, showing the optimized method as a practical alternative to produce valuable lipids for food and nutraceutical applications.https://www.mdpi.com/1420-3049/25/14/3310omega-3 PUFAmicroalgaeadvanced extraction techniquespressurized liquid extraction (PLE)enzymatic pre-treatment<i>Isochrysis galbana</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María Señoráns Natalia Castejón Francisco Javier Señoráns |
spellingShingle |
María Señoráns Natalia Castejón Francisco Javier Señoráns Advanced Extraction of Lipids with DHA from <i>Isochrysis galbana</i> with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions Molecules omega-3 PUFA microalgae advanced extraction techniques pressurized liquid extraction (PLE) enzymatic pre-treatment <i>Isochrysis galbana</i> |
author_facet |
María Señoráns Natalia Castejón Francisco Javier Señoráns |
author_sort |
María Señoráns |
title |
Advanced Extraction of Lipids with DHA from <i>Isochrysis galbana</i> with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions |
title_short |
Advanced Extraction of Lipids with DHA from <i>Isochrysis galbana</i> with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions |
title_full |
Advanced Extraction of Lipids with DHA from <i>Isochrysis galbana</i> with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions |
title_fullStr |
Advanced Extraction of Lipids with DHA from <i>Isochrysis galbana</i> with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions |
title_full_unstemmed |
Advanced Extraction of Lipids with DHA from <i>Isochrysis galbana</i> with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions |
title_sort |
advanced extraction of lipids with dha from <i>isochrysis galbana</i> with enzymatic pre-treatment combined with pressurized liquids and ultrasound assisted extractions |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-07-01 |
description |
Microalgal biomass is a sustainable and valuable source of lipids with omega-3 fatty acids. The efficient extraction of lipids from microalgae requires fast and alternative extraction methods, frequently combined with biomass pre-treatment by different procedures. In this work, Pressurized liquid extraction (PLE) was optimized and compared with traditional lipid extraction methods, Folch and Bligh and Dyer, and with a new Ultrasound Assisted Extraction (UAE) method for lipids from microalgae <i>Isochrysis galbana</i>. To further optimize PLE and UAE, enzymatic pre-treatment of microalga <i>Isochrysis galbana</i> was studied with commercial enzymes Viscozyme and Celluclast. No significant differences were found for lipid yields among different extraction techniques used. However, advanced extraction techniques with or without pre-treatment are a green, fast, and toxic solvent free alternative to traditional techniques. Lipid composition of <i>Isochrysis</i> was determined by HPLC-ELSD and included neutral and polar lipids, showing that each fraction comprised different contents in omega-3 polyunsaturated fatty acids (PUFA). The highest polar lipids content was achieved with UAE (50 °C and 15 min) and PLE (100 °C) techniques. Moreover, the highest omega-3 PUFA (33.2%), eicosapentaenoic acid (EPA) (3.3%) and docosahexaenoic acid (DHA) (12.0%) contents were achieved with the advanced technique UAE, showing the optimized method as a practical alternative to produce valuable lipids for food and nutraceutical applications. |
topic |
omega-3 PUFA microalgae advanced extraction techniques pressurized liquid extraction (PLE) enzymatic pre-treatment <i>Isochrysis galbana</i> |
url |
https://www.mdpi.com/1420-3049/25/14/3310 |
work_keys_str_mv |
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