Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran
Background: Cystic Echinococcosis (CE) is one of the most common parasitic zoonosis caused by Echinococcus granulosus worldwide. This study investigated genotype diversity of Echinococcus granulosus isolated from stray dogs and golden jackals in Ilam province, West of Iran. Methods: Adult worms we...
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Tehran University of Medical Sciences
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doaj-cbd6061c6b634d1daa06db9543ec4dc32021-03-02T05:01:52ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2017-12-01124712Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of IranAbdolhossein DALIMI0Morteza SHAMSI1Afra KHOSRAVI2Fatemeh GHAFFARIFAR3Dept. of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDept. of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDept. of Immunology, School of Medicine, Ilam University of Medical Sciences, Ilam, IranDept. of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Background: Cystic Echinococcosis (CE) is one of the most common parasitic zoonosis caused by Echinococcus granulosus worldwide. This study investigated genotype diversity of Echinococcus granulosus isolated from stray dogs and golden jackals in Ilam province, West of Iran. Methods: Adult worms were collected from the small intestine of the stray dogs and golden jackals from Ilam Province roads during 2012-2014. DNA was extracted from the adult worms and the partial mitochondrial NADH dehydrogenase subunit1 (nad1) was amplified by PCR then the products were digested by using HpaII, Rsa1, and Alu1 restriction enzymes. In order to confirm RFLP results, a number of PCR products were bi-directionally sequenced. Results: Totally, 20 stray dogs out of 75 (26.66%) and two out of 73 (2.74%) golden jackal showed infection with E. granulosus. Amplified PCR product for all isolates was a band of approximately 550bp. Alu1 digested the product into two bands of approximately 160bp and 390bp fragments, while the Rsa1 cut the product into 320bp and 230bp fragments and the HpaII had no effect on the PCR product for both dog and jackal samples. The isolate sequences of mtDNAnad1 gene indicated 100% homology with references G1, G2 and G3 sequences in the GenBank database. Conclusion: The genotype of adult E. granulosus was similar to larval stage genotypes of parasite (G1-G3 complex). https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1936Echinococcus granulosusStray dogGolden jackalGenotypingIran |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abdolhossein DALIMI Morteza SHAMSI Afra KHOSRAVI Fatemeh GHAFFARIFAR |
spellingShingle |
Abdolhossein DALIMI Morteza SHAMSI Afra KHOSRAVI Fatemeh GHAFFARIFAR Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran Iranian Journal of Parasitology Echinococcus granulosus Stray dog Golden jackal Genotyping Iran |
author_facet |
Abdolhossein DALIMI Morteza SHAMSI Afra KHOSRAVI Fatemeh GHAFFARIFAR |
author_sort |
Abdolhossein DALIMI |
title |
Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran |
title_short |
Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran |
title_full |
Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran |
title_fullStr |
Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran |
title_full_unstemmed |
Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran |
title_sort |
genotyping echinococcus granulosus from canine isolates in ilam province, west of iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2017-12-01 |
description |
Background: Cystic Echinococcosis (CE) is one of the most common parasitic zoonosis caused by Echinococcus granulosus worldwide. This study investigated genotype diversity of Echinococcus granulosus isolated from stray dogs and golden jackals in Ilam province, West of Iran.
Methods: Adult worms were collected from the small intestine of the stray dogs and golden jackals from Ilam Province roads during 2012-2014. DNA was extracted from the adult worms and the partial mitochondrial NADH dehydrogenase subunit1 (nad1) was amplified by PCR then the products were digested by using HpaII, Rsa1, and Alu1 restriction enzymes. In order to confirm RFLP results, a number of PCR products were bi-directionally sequenced.
Results: Totally, 20 stray dogs out of 75 (26.66%) and two out of 73 (2.74%) golden jackal showed infection with E. granulosus. Amplified PCR product for all isolates was a band of approximately 550bp. Alu1 digested the product into two bands of approximately 160bp and 390bp fragments, while the Rsa1 cut the product into 320bp and 230bp fragments and the HpaII had no effect on the PCR product for both dog and jackal samples. The isolate sequences of mtDNAnad1 gene indicated 100% homology with references G1, G2 and G3 sequences in the GenBank database.
Conclusion: The genotype of adult E. granulosus was similar to larval stage genotypes of parasite (G1-G3 complex).
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topic |
Echinococcus granulosus Stray dog Golden jackal Genotyping Iran |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1936 |
work_keys_str_mv |
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