Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage
Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2014-03-01
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| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/journal/view.html?uid=28&sort=&scale=&key=year&keyword=&s_v=20&s_n=1&pn=vol&year=2014&vmd=Full |
| Summary: | Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging
diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and
accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae
DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In
sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml
of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time
PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific
primer set could be useful for rapid and accurate detection of P. brassicae. |
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| ISSN: | 1598-2262 2233-9191 |