Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage

• Main Authors: Jin Su Choi, Seul Gi Yang, Jeong Young Song, Hong Gi Kim
• Format: Article
• Language: English
• Published: Hanrimwon Publishing Company 2014-03-01
• Series: Research in Plant Disease
• Subjects: Clubroot pathogen; Detection; Plasmodiophora brassicae; Quantitative real-time PCR
• Online Access: http://www.online-rpd.org/journal/view.html?uid=28&sort=&scale=&key=year&keyword=&s_v=20&s_n=1&pn=vol&year=2014&vmd=Full

Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae.