Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5
CD44 is a transmembrane glycoprotein that regulates a variety of genes related to cell-adhesion, migration, proliferation, differentiation, and survival. A large number of alternative splicing isoforms of CD44, containing various combinations of alternative exons, have been reported. CD44 standard (...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2018-07-01
|
Series: | Biochemistry and Biophysics Reports |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580818300670 |
id |
doaj-cbe60391c90741f7a9ca4f379c5b725f |
---|---|
record_format |
Article |
spelling |
doaj-cbe60391c90741f7a9ca4f379c5b725f2020-11-24T21:18:56ZengElsevierBiochemistry and Biophysics Reports2405-58082018-07-0114C646810.1016/j.bbrep.2018.03.007Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5Shinji Yamada0Shunsuke Itai1Takuro Nakamura2Miyuki Yanaka3Mika K. Kaneko4Yukinari Kato5Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanCD44 is a transmembrane glycoprotein that regulates a variety of genes related to cell-adhesion, migration, proliferation, differentiation, and survival. A large number of alternative splicing isoforms of CD44, containing various combinations of alternative exons, have been reported. CD44 standard (CD44s), which lacks variant exons, is widely expressed on the surface of most tissues and all hematopoietic cells. In contrast, CD44 variant isoforms show tissue-specific expression patterns and have been extensively studied as both prognostic markers and therapeutic targets in cancer and other diseases. In this study, we immunized mice with CHO-K1 cell lines overexpressing CD44v3-10 to obtain novel anti-CD44 mAbs. One of the clones, C44Mab-5 (IgG1, kappa), recognized both CD44s and CD44v3-10. C44Mab-5 also reacted with oral cancer cells such as Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4 using flow cytometry. Moreover, immunohistochemical analysis revealed that C44Mab-5 detected 166/182 (91.2%) of oral cancers. These results suggest that the C44Mab-5 antibody may be useful for investigating the expression and function of CD44 in various cancers.http://www.sciencedirect.com/science/article/pii/S2405580818300670CD44Monoclonal antibodyImmunohistochemistryOral cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shinji Yamada Shunsuke Itai Takuro Nakamura Miyuki Yanaka Mika K. Kaneko Yukinari Kato |
spellingShingle |
Shinji Yamada Shunsuke Itai Takuro Nakamura Miyuki Yanaka Mika K. Kaneko Yukinari Kato Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5 Biochemistry and Biophysics Reports CD44 Monoclonal antibody Immunohistochemistry Oral cancer |
author_facet |
Shinji Yamada Shunsuke Itai Takuro Nakamura Miyuki Yanaka Mika K. Kaneko Yukinari Kato |
author_sort |
Shinji Yamada |
title |
Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5 |
title_short |
Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5 |
title_full |
Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5 |
title_fullStr |
Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5 |
title_full_unstemmed |
Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5 |
title_sort |
detection of high cd44 expression in oral cancers using the novel monoclonal antibody, c44mab-5 |
publisher |
Elsevier |
series |
Biochemistry and Biophysics Reports |
issn |
2405-5808 |
publishDate |
2018-07-01 |
description |
CD44 is a transmembrane glycoprotein that regulates a variety of genes related to cell-adhesion, migration, proliferation, differentiation, and survival. A large number of alternative splicing isoforms of CD44, containing various combinations of alternative exons, have been reported. CD44 standard (CD44s), which lacks variant exons, is widely expressed on the surface of most tissues and all hematopoietic cells. In contrast, CD44 variant isoforms show tissue-specific expression patterns and have been extensively studied as both prognostic markers and therapeutic targets in cancer and other diseases. In this study, we immunized mice with CHO-K1 cell lines overexpressing CD44v3-10 to obtain novel anti-CD44 mAbs. One of the clones, C44Mab-5 (IgG1, kappa), recognized both CD44s and CD44v3-10. C44Mab-5 also reacted with oral cancer cells such as Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4 using flow cytometry. Moreover, immunohistochemical analysis revealed that C44Mab-5 detected 166/182 (91.2%) of oral cancers. These results suggest that the C44Mab-5 antibody may be useful for investigating the expression and function of CD44 in various cancers. |
topic |
CD44 Monoclonal antibody Immunohistochemistry Oral cancer |
url |
http://www.sciencedirect.com/science/article/pii/S2405580818300670 |
work_keys_str_mv |
AT shinjiyamada detectionofhighcd44expressioninoralcancersusingthenovelmonoclonalantibodyc44mab5 AT shunsukeitai detectionofhighcd44expressioninoralcancersusingthenovelmonoclonalantibodyc44mab5 AT takuronakamura detectionofhighcd44expressioninoralcancersusingthenovelmonoclonalantibodyc44mab5 AT miyukiyanaka detectionofhighcd44expressioninoralcancersusingthenovelmonoclonalantibodyc44mab5 AT mikakkaneko detectionofhighcd44expressioninoralcancersusingthenovelmonoclonalantibodyc44mab5 AT yukinarikato detectionofhighcd44expressioninoralcancersusingthenovelmonoclonalantibodyc44mab5 |
_version_ |
1726007638562439168 |