Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus

Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus

Although primary cultures of neurons are essential methods for cell biological and pharmacological researches, many animals must be sacrificed for each experiment. Here we introduce a novel system to cryopreserve hippocampal granule cells (GCs) prepared from postnatal rats. Being thawed after as lon...

Full description

Bibliographic Details
Main Authors: Junya Ichikawa, Ryuji X Yamada, Rieko Muramatsu, Yuji Ikegaya, Norio Matsuki, Ryuta Koyama
Format: Article
Language:English
Published: Elsevier 2007-01-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319342380
id doaj-cbe89a8e8f74450987b6f1e6f13da1ee
record_format Article
spelling doaj-cbe89a8e8f74450987b6f1e6f13da1ee2020-11-25T01:26:21ZengElsevierJournal of Pharmacological Sciences1347-86132007-01-011044387391Cryopreservation of Granule Cells From the Postnatal Rat HippocampusJunya Ichikawa0Ryuji X Yamada1Rieko Muramatsu2Yuji Ikegaya3Norio Matsuki4Ryuta Koyama5Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, JapanLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, JapanLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, JapanLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, JapanLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, JapanLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan; Corresponding author. rkoyama@mol.f.u-tokyo.ac.jpAlthough primary cultures of neurons are essential methods for cell biological and pharmacological researches, many animals must be sacrificed for each experiment. Here we introduce a novel system to cryopreserve hippocampal granule cells (GCs) prepared from postnatal rats. Being thawed after as long as 60 days of cryopreservation, GCs expressed the mature neuronal marker MAP-2 and elongated single tau-1-positive axons and multiple tau-1-negative dendrites. These properties closely resembled intact GCs in primary cultures, providing the advantage of being able to repeatedly prepare stable cultures with a single sacrifice of animals. Keywords:: dentate gyrus, primary culture, Prox1http://www.sciencedirect.com/science/article/pii/S1347861319342380
collection DOAJ
language English
format Article
sources DOAJ
author Junya Ichikawa
Ryuji X Yamada
Rieko Muramatsu
Yuji Ikegaya
Norio Matsuki
Ryuta Koyama
spellingShingle Junya Ichikawa
Ryuji X Yamada
Rieko Muramatsu
Yuji Ikegaya
Norio Matsuki
Ryuta Koyama
Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus
Journal of Pharmacological Sciences
author_facet Junya Ichikawa
Ryuji X Yamada
Rieko Muramatsu
Yuji Ikegaya
Norio Matsuki
Ryuta Koyama
author_sort Junya Ichikawa
title Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus
title_short Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus
title_full Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus
title_fullStr Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus
title_full_unstemmed Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus
title_sort cryopreservation of granule cells from the postnatal rat hippocampus
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2007-01-01
description Although primary cultures of neurons are essential methods for cell biological and pharmacological researches, many animals must be sacrificed for each experiment. Here we introduce a novel system to cryopreserve hippocampal granule cells (GCs) prepared from postnatal rats. Being thawed after as long as 60 days of cryopreservation, GCs expressed the mature neuronal marker MAP-2 and elongated single tau-1-positive axons and multiple tau-1-negative dendrites. These properties closely resembled intact GCs in primary cultures, providing the advantage of being able to repeatedly prepare stable cultures with a single sacrifice of animals. Keywords:: dentate gyrus, primary culture, Prox1
url http://www.sciencedirect.com/science/article/pii/S1347861319342380
work_keys_str_mv AT junyaichikawa cryopreservationofgranulecellsfromthepostnatalrathippocampus
AT ryujixyamada cryopreservationofgranulecellsfromthepostnatalrathippocampus
AT riekomuramatsu cryopreservationofgranulecellsfromthepostnatalrathippocampus
AT yujiikegaya cryopreservationofgranulecellsfromthepostnatalrathippocampus
AT noriomatsuki cryopreservationofgranulecellsfromthepostnatalrathippocampus
AT ryutakoyama cryopreservationofgranulecellsfromthepostnatalrathippocampus
_version_ 1725109425027416064

Similar Items