Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy
Abstract Background This study aimed to determine early postoperative changes of serum sphingomyelin (SM) and ceramide (CER) species following laparoscopic sleeve gastrectomy (LSG). Methods Twenty obese patients [mean body mass index (BMI) 45,64 ± 6,10 kg/m2] underwent LSG and normal weight control...
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doaj-cbece08276014fdeb740444ee1cc575d2020-11-25T02:02:21ZengBMCLipids in Health and Disease1476-511X2018-11-0117111110.1186/s12944-018-0917-zEarly postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomyHakan Özer0İbrahim Aslan1Mehmet Tahir Oruç2Yaşar Çöpelci3Ebru Afşar4Sabriye Kaya5Mutay Aslan6Internal Medicine Clinic, Antalya Research and Education HospitalEndocrinology Clinic, SBU Antalya Research and Education HospitalSurgery Clinic, Antalya Research and Education HospitalSurgery Clinic, Antalya Research and Education HospitalDepartment of Medical Biochemistry, Akdeniz University Medical FacultyDepartment of Medical Biochemistry, Akdeniz University Medical FacultyDepartment of Medical Biochemistry, Akdeniz University Medical FacultyAbstract Background This study aimed to determine early postoperative changes of serum sphingomyelin (SM) and ceramide (CER) species following laparoscopic sleeve gastrectomy (LSG). Methods Twenty obese patients [mean body mass index (BMI) 45,64 ± 6,10 kg/m2] underwent LSG and normal weight control patients (mean BMI 31,51 ± 6,21 kg/m2) underwent laparoscopic cholecystectomy. Fasting blood samples were collected prior to surgery, at day 1 and day 30 after surgery. Circulating levels of C16-C24 SMs, C16-C24 CERs and sphingosine-1-phosphate (S1P) were determined by an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Serum activity of neutral sphingomyelinase (N-SMase) was assayed by standard kit methods, and ceramide-1-phosphate (C1P) levels were determined by enzyme-linked immunosorbent assay (ELISA). Lipid profile, routine biochemical and hormone parameters were assayed by standard kit methods. Insulin sensitivity was evaluated using homeostatic model assessment for insulin resistance (HOMA IR). Results A significant decrease was observed in serum levels of very-long-chain C24 SM, very-long-chain C22-C24 CERs, HOMA-IR, N-SMase and C1P in LSG patients after postoperation day 1 and day 30 compared to preoperation levels. At 30 days postsurgery, BMI was reduced by 11%, fasting triglycerides were significantly decreased, and insulin sensitivity was increased compared to presurgery values. A significant positive correlation was found between HOMA-IR and serum levels of C22-C24 CERs in LSG patients. Conclusion We conclude that very long chain CERs may mediate improved insulin sensitivity after LSG.http://link.springer.com/article/10.1186/s12944-018-0917-zLaparoscopic sleeve gastrectomySphingomyelinCeramide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hakan Özer İbrahim Aslan Mehmet Tahir Oruç Yaşar Çöpelci Ebru Afşar Sabriye Kaya Mutay Aslan |
spellingShingle |
Hakan Özer İbrahim Aslan Mehmet Tahir Oruç Yaşar Çöpelci Ebru Afşar Sabriye Kaya Mutay Aslan Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy Lipids in Health and Disease Laparoscopic sleeve gastrectomy Sphingomyelin Ceramide |
author_facet |
Hakan Özer İbrahim Aslan Mehmet Tahir Oruç Yaşar Çöpelci Ebru Afşar Sabriye Kaya Mutay Aslan |
author_sort |
Hakan Özer |
title |
Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy |
title_short |
Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy |
title_full |
Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy |
title_fullStr |
Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy |
title_full_unstemmed |
Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy |
title_sort |
early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy |
publisher |
BMC |
series |
Lipids in Health and Disease |
issn |
1476-511X |
publishDate |
2018-11-01 |
description |
Abstract Background This study aimed to determine early postoperative changes of serum sphingomyelin (SM) and ceramide (CER) species following laparoscopic sleeve gastrectomy (LSG). Methods Twenty obese patients [mean body mass index (BMI) 45,64 ± 6,10 kg/m2] underwent LSG and normal weight control patients (mean BMI 31,51 ± 6,21 kg/m2) underwent laparoscopic cholecystectomy. Fasting blood samples were collected prior to surgery, at day 1 and day 30 after surgery. Circulating levels of C16-C24 SMs, C16-C24 CERs and sphingosine-1-phosphate (S1P) were determined by an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Serum activity of neutral sphingomyelinase (N-SMase) was assayed by standard kit methods, and ceramide-1-phosphate (C1P) levels were determined by enzyme-linked immunosorbent assay (ELISA). Lipid profile, routine biochemical and hormone parameters were assayed by standard kit methods. Insulin sensitivity was evaluated using homeostatic model assessment for insulin resistance (HOMA IR). Results A significant decrease was observed in serum levels of very-long-chain C24 SM, very-long-chain C22-C24 CERs, HOMA-IR, N-SMase and C1P in LSG patients after postoperation day 1 and day 30 compared to preoperation levels. At 30 days postsurgery, BMI was reduced by 11%, fasting triglycerides were significantly decreased, and insulin sensitivity was increased compared to presurgery values. A significant positive correlation was found between HOMA-IR and serum levels of C22-C24 CERs in LSG patients. Conclusion We conclude that very long chain CERs may mediate improved insulin sensitivity after LSG. |
topic |
Laparoscopic sleeve gastrectomy Sphingomyelin Ceramide |
url |
http://link.springer.com/article/10.1186/s12944-018-0917-z |
work_keys_str_mv |
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