DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian Cells

Silver has a long history of antibacterial effectiveness. The combination of atomically precise metal nanoclusters with the field of nucleic acid nanotechnology has given rise to DNA-templated silver nanoclusters (DNA-AgNCs) which can be engineered with reproducible and unique fluorescent properties...

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Main Authors:	Lewis Rolband, Liam Yourston, Morgan Chandler, Damian Beasock, Leyla Danai, Seraphim Kozlov, Nolan Marshall, Oleg Shevchenko, Alexey V. Krasnoslobodtsev, Kirill A. Afonin
Format:	Article
Language:	English
Published:	MDPI AG 2021-07-01
Series:	Molecules
Subjects:	DNA silver AgNC nanocluster antibacterial fluorescence
Online Access:	https://www.mdpi.com/1420-3049/26/13/4045

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spelling	doaj-cc0d91abe1244d9f8ba6bceacf5d91f12021-07-15T15:42:53ZengMDPI AGMolecules1420-30492021-07-01264045404510.3390/molecules26134045DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian CellsLewis Rolband0Liam Yourston1Morgan Chandler2Damian Beasock3Leyla Danai4Seraphim Kozlov5Nolan Marshall6Oleg Shevchenko7Alexey V. Krasnoslobodtsev8Kirill A. Afonin9Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USADepartment of Physics, University of Nebraska at Omaha, Omaha, NE 68182, USANanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USANanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USANanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USANanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USADepartment of Physics, University of Nebraska at Omaha, Omaha, NE 68182, USANanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USADepartment of Physics, University of Nebraska at Omaha, Omaha, NE 68182, USANanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USASilver has a long history of antibacterial effectiveness. The combination of atomically precise metal nanoclusters with the field of nucleic acid nanotechnology has given rise to DNA-templated silver nanoclusters (DNA-AgNCs) which can be engineered with reproducible and unique fluorescent properties and antibacterial activity. Furthermore, cytosine-rich single-stranded DNA oligonucleotides designed to fold into hairpin structures improve the stability of AgNCs and additionally modulate their antibacterial properties and the quality of observed fluorescent signals. In this work, we characterize the sequence-specific fluorescence and composition of four representative DNA-AgNCs, compare their corresponding antibacterial effectiveness at different pH, and assess cytotoxicity to several mammalian cell lines.https://www.mdpi.com/1420-3049/26/13/4045DNAsilverAgNCnanoclusterantibacterialfluorescence
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Lewis Rolband Liam Yourston Morgan Chandler Damian Beasock Leyla Danai Seraphim Kozlov Nolan Marshall Oleg Shevchenko Alexey V. Krasnoslobodtsev Kirill A. Afonin
spellingShingle	Lewis Rolband Liam Yourston Morgan Chandler Damian Beasock Leyla Danai Seraphim Kozlov Nolan Marshall Oleg Shevchenko Alexey V. Krasnoslobodtsev Kirill A. Afonin DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian Cells Molecules DNA silver AgNC nanocluster antibacterial fluorescence
author_facet	Lewis Rolband Liam Yourston Morgan Chandler Damian Beasock Leyla Danai Seraphim Kozlov Nolan Marshall Oleg Shevchenko Alexey V. Krasnoslobodtsev Kirill A. Afonin
author_sort	Lewis Rolband
title	DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian Cells
title_short	DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian Cells
title_full	DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian Cells
title_fullStr	DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian Cells
title_full_unstemmed	DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian Cells
title_sort	dna-templated fluorescent silver nanoclusters inhibit bacterial growth while being non-toxic to mammalian cells
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2021-07-01
description	Silver has a long history of antibacterial effectiveness. The combination of atomically precise metal nanoclusters with the field of nucleic acid nanotechnology has given rise to DNA-templated silver nanoclusters (DNA-AgNCs) which can be engineered with reproducible and unique fluorescent properties and antibacterial activity. Furthermore, cytosine-rich single-stranded DNA oligonucleotides designed to fold into hairpin structures improve the stability of AgNCs and additionally modulate their antibacterial properties and the quality of observed fluorescent signals. In this work, we characterize the sequence-specific fluorescence and composition of four representative DNA-AgNCs, compare their corresponding antibacterial effectiveness at different pH, and assess cytotoxicity to several mammalian cell lines.
topic	DNA silver AgNC nanocluster antibacterial fluorescence
url	https://www.mdpi.com/1420-3049/26/13/4045
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DNA-Templated Fluorescent Silver Nanoclusters Inhibit Bacterial Growth While Being Non-Toxic to Mammalian Cells

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