Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expression
Abstract Background Schisandra chinenesis (SC) has been reported to have ameliorative effect on osteoporosis. However, the mechanisms underlying the anti-osteoporosis activity of SC have not been clearly elucidated. In the present study, we determined the effects of SC on The receptor activator of N...
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doaj-cc1f109585154485813a138ab68f5f072020-11-25T02:42:42ZengBMCBMC Complementary and Alternative Medicine1472-68822018-10-011811810.1186/s12906-018-2331-5Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expressionEun-Jung Kim0Haesu Lee1Mi Hye Kim2Woong Mo Yang3College of Korean Medicine, Dongguk UniversityDepartment of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee UniversityDepartment of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee UniversityDepartment of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee UniversityAbstract Background Schisandra chinenesis (SC) has been reported to have ameliorative effect on osteoporosis. However, the mechanisms underlying the anti-osteoporosis activity of SC have not been clearly elucidated. In the present study, we determined the effects of SC on The receptor activator of NF-kB ligand (RANKL)-induced osteoclastogenesis and its potential mechanism. Methods Raw 264.7 cells were treated with 0.6, 6 and 60 μg/mL SC in the presence of 100 ng/mL RANKL for 7 days. RANKL-induced osteoclast formation was analyzed by tartrate resistant acid phosphatase (TRAP) staining. The osteoclast differentiation-related factors were confirmed along with TNF-α. Results SC inhibits the RANKL-induced osteoclast differentiation in dose-dependent manner within non-toxic concentrations. The supernatant concentrations of TNF-α were significantly decreased by SC treatment. In addition, osteoclastogenesis-related factors, TRAP6 and NF-κB, were markedly decreased by SC in RANKL-induced osteoclasts. Mechanistically, SC reduced the RANKL-triggered NFATc1 and c-fos expressions. Conclusions Taken together, our data suggest that SC can modulate bone metabolism by suppressing RANKL-induced osteoclast differentiation.http://link.springer.com/article/10.1186/s12906-018-2331-5Schisandra chinensisOsteoclastogenesisRANKLNFATc1C-fos |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eun-Jung Kim Haesu Lee Mi Hye Kim Woong Mo Yang |
spellingShingle |
Eun-Jung Kim Haesu Lee Mi Hye Kim Woong Mo Yang Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expression BMC Complementary and Alternative Medicine Schisandra chinensis Osteoclastogenesis RANKL NFATc1 C-fos |
author_facet |
Eun-Jung Kim Haesu Lee Mi Hye Kim Woong Mo Yang |
author_sort |
Eun-Jung Kim |
title |
Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expression |
title_short |
Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expression |
title_full |
Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expression |
title_fullStr |
Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expression |
title_full_unstemmed |
Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expression |
title_sort |
inhibition of rankl-stimulated osteoclast differentiation by schisandra chinensis through down-regulation of nfatc1 and c-fos expression |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2018-10-01 |
description |
Abstract Background Schisandra chinenesis (SC) has been reported to have ameliorative effect on osteoporosis. However, the mechanisms underlying the anti-osteoporosis activity of SC have not been clearly elucidated. In the present study, we determined the effects of SC on The receptor activator of NF-kB ligand (RANKL)-induced osteoclastogenesis and its potential mechanism. Methods Raw 264.7 cells were treated with 0.6, 6 and 60 μg/mL SC in the presence of 100 ng/mL RANKL for 7 days. RANKL-induced osteoclast formation was analyzed by tartrate resistant acid phosphatase (TRAP) staining. The osteoclast differentiation-related factors were confirmed along with TNF-α. Results SC inhibits the RANKL-induced osteoclast differentiation in dose-dependent manner within non-toxic concentrations. The supernatant concentrations of TNF-α were significantly decreased by SC treatment. In addition, osteoclastogenesis-related factors, TRAP6 and NF-κB, were markedly decreased by SC in RANKL-induced osteoclasts. Mechanistically, SC reduced the RANKL-triggered NFATc1 and c-fos expressions. Conclusions Taken together, our data suggest that SC can modulate bone metabolism by suppressing RANKL-induced osteoclast differentiation. |
topic |
Schisandra chinensis Osteoclastogenesis RANKL NFATc1 C-fos |
url |
http://link.springer.com/article/10.1186/s12906-018-2331-5 |
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