Electrochemically Activated Screen-Printed Carbon Sensor Modified with Anionic Surfactant (aSPCE/SDS) for Simultaneous Determination of Paracetamol, Diclofenac and Tramadol

• Main Authors: Jędrzej Kozak, Katarzyna Tyszczuk-Rotko, Magdalena Wójciak, Ireneusz Sowa
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: Materials
• Subjects: electrochemically activated screen-printed carbon electrode modified with sodium dodecyl sulfate; simultaneous determination of paracetamol; diclofenac and tramadol; differential pulse adsorptive stripping voltammetry; river water; human serum and pharmaceutical formulation samples
• Online Access: https://www.mdpi.com/1996-1944/14/13/3581
• ISSN: 1996-1944

In this work, an electrochemically activated screen-printed carbon electrode modified with sodium dodecyl sulfate (aSPCE/SDS) was proposed for the simultaneous determination of paracetamol (PA), diclofenac (DF), and tramadol (TR). Changes of surface morphology and electrochemical behaviour of the electrode after the electrochemical activation with H₂O₂ and SDS surface modification were studied by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The influence of various parameters on the responses of the aSPCE/SDS such as pH and concentration of the buffer, SDS concentration, and techniques parameters were investigated. Using optimised conditions (E_acc. of −0.4 V, t_acc. of 120 s, ΔE_A of 150 mV, ν of 250 mV s⁻¹, and t_m of 10 ms), the aSPCE/SDS showed a good linear response in the concentration ranges of 5.0 × 10⁻⁸–2.0 × 10⁻⁵ for PA, 1.0 × 10⁻⁹–2.0 × 10⁻⁷ for DF, and 1.0 × 10⁻⁸–2.0 × 10⁻⁷ and 2.0 × 10⁻⁷–2.0 × 10⁻⁶ mol L⁻¹ for TR. The limits of detection obtained during the simultaneous determination of PA, DF, and TR are 1.49 × 10⁻⁸ mol L⁻¹, 2.10 × 10⁻¹⁰ mol L⁻¹, and 1.71 × 10⁻⁹ mol L⁻¹, respectively. The selectivity of the aSPCE/SDS was evaluated by examination of the impact of some inorganic and organic substances that are commonly present in environmental and biological samples on the responses of PA, DF, and TR. Finally, the differential pulse adsorptive stripping voltammetric (DPAdSV) procedure using the aSPCE/SDS was successfully applied for the determination of PA, DF, and TR in river water and serum samples as well as pharmaceuticals.