Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring
The aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccases, which were secreted in media containing environmental wastes, were characterized for biochemical and kinetic novelty and applied in the decol...
Main Author: | |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2020-03-01
|
Series: | Biotechnology Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X19304473 |
id |
doaj-cc340e59eb0e475aa26ae32faaca0a7f |
---|---|
record_format |
Article |
spelling |
doaj-cc340e59eb0e475aa26ae32faaca0a7f2020-11-25T02:27:36ZengElsevierBiotechnology Reports2215-017X2020-03-0125Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouringJohn O. Unuofin0SA-MRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice, 5700, South Africa; Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice, 5700, South Africa; Department of Environmental, Water and Earth Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa; Corresponding author at: Department of Environmental, Water and Earth Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South AfricaThe aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccases, which were secreted in media containing environmental wastes, were characterized for biochemical and kinetic novelty and applied in the decolourization of some synthetic dyes and subsequently, denim bleaching. The laccases exhibited enhanced pH-, thermo-, psychro-, metal-, halo-, and surfacto-tolerance, eliciting residual activities of at least ca. 71%. Thereafter, the enzymes were able to decolourize novel high concentrations of synthetic dyes (0.2% w v−1) at 56 h of incubation, and also elicit a mediator-assisted perpetual wash up and decolourization of indigo pigment from fabric under 6 h. The outcomes observed in this study therefore warrant the adoption of these isolates for applications toward a sustainable and total environment through production of fine biochemicals, and the minimization of environmental wastes. Keywords: Environmental sustainability, Dye decolourization, Denim bleaching, Laccasehttp://www.sciencedirect.com/science/article/pii/S2215017X19304473 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
John O. Unuofin |
spellingShingle |
John O. Unuofin Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring Biotechnology Reports |
author_facet |
John O. Unuofin |
author_sort |
John O. Unuofin |
title |
Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring |
title_short |
Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring |
title_full |
Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring |
title_fullStr |
Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring |
title_full_unstemmed |
Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring |
title_sort |
sustainability potentials of novel laccase tinctures from stenotrophomonas maltophilia bij16 and bordetella bronchiseptica hso16: from dye decolourization to denim bioscouring |
publisher |
Elsevier |
series |
Biotechnology Reports |
issn |
2215-017X |
publishDate |
2020-03-01 |
description |
The aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccases, which were secreted in media containing environmental wastes, were characterized for biochemical and kinetic novelty and applied in the decolourization of some synthetic dyes and subsequently, denim bleaching. The laccases exhibited enhanced pH-, thermo-, psychro-, metal-, halo-, and surfacto-tolerance, eliciting residual activities of at least ca. 71%. Thereafter, the enzymes were able to decolourize novel high concentrations of synthetic dyes (0.2% w v−1) at 56 h of incubation, and also elicit a mediator-assisted perpetual wash up and decolourization of indigo pigment from fabric under 6 h. The outcomes observed in this study therefore warrant the adoption of these isolates for applications toward a sustainable and total environment through production of fine biochemicals, and the minimization of environmental wastes. Keywords: Environmental sustainability, Dye decolourization, Denim bleaching, Laccase |
url |
http://www.sciencedirect.com/science/article/pii/S2215017X19304473 |
work_keys_str_mv |
AT johnounuofin sustainabilitypotentialsofnovellaccasetincturesfromstenotrophomonasmaltophiliabij16andbordetellabronchisepticahso16fromdyedecolourizationtodenimbioscouring |
_version_ |
1724842134663593984 |