Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring

Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring

The aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccases, which were secreted in media containing environmental wastes, were characterized for biochemical and kinetic novelty and applied in the decol...

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Main Author: John O. Unuofin
Format: Article
Language:English
Published: Elsevier 2020-03-01
Series:Biotechnology Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X19304473
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spelling doaj-cc340e59eb0e475aa26ae32faaca0a7f2020-11-25T02:27:36ZengElsevierBiotechnology Reports2215-017X2020-03-0125Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouringJohn O. Unuofin0SA-MRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice, 5700, South Africa; Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice, 5700, South Africa; Department of Environmental, Water and Earth Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa; Corresponding author at: Department of Environmental, Water and Earth Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South AfricaThe aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccases, which were secreted in media containing environmental wastes, were characterized for biochemical and kinetic novelty and applied in the decolourization of some synthetic dyes and subsequently, denim bleaching. The laccases exhibited enhanced pH-, thermo-, psychro-, metal-, halo-, and surfacto-tolerance, eliciting residual activities of at least ca. 71%. Thereafter, the enzymes were able to decolourize novel high concentrations of synthetic dyes (0.2% w v−1) at 56 h of incubation, and also elicit a mediator-assisted perpetual wash up and decolourization of indigo pigment from fabric under 6 h. The outcomes observed in this study therefore warrant the adoption of these isolates for applications toward a sustainable and total environment through production of fine biochemicals, and the minimization of environmental wastes. Keywords: Environmental sustainability, Dye decolourization, Denim bleaching, Laccasehttp://www.sciencedirect.com/science/article/pii/S2215017X19304473
collection DOAJ
language English
format Article
sources DOAJ
author John O. Unuofin
spellingShingle John O. Unuofin
Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring
Biotechnology Reports
author_facet John O. Unuofin
author_sort John O. Unuofin
title Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring
title_short Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring
title_full Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring
title_fullStr Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring
title_full_unstemmed Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring
title_sort sustainability potentials of novel laccase tinctures from stenotrophomonas maltophilia bij16 and bordetella bronchiseptica hso16: from dye decolourization to denim bioscouring
publisher Elsevier
series Biotechnology Reports
issn 2215-017X
publishDate 2020-03-01
description The aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccases, which were secreted in media containing environmental wastes, were characterized for biochemical and kinetic novelty and applied in the decolourization of some synthetic dyes and subsequently, denim bleaching. The laccases exhibited enhanced pH-, thermo-, psychro-, metal-, halo-, and surfacto-tolerance, eliciting residual activities of at least ca. 71%. Thereafter, the enzymes were able to decolourize novel high concentrations of synthetic dyes (0.2% w v−1) at 56 h of incubation, and also elicit a mediator-assisted perpetual wash up and decolourization of indigo pigment from fabric under 6 h. The outcomes observed in this study therefore warrant the adoption of these isolates for applications toward a sustainable and total environment through production of fine biochemicals, and the minimization of environmental wastes. Keywords: Environmental sustainability, Dye decolourization, Denim bleaching, Laccase
url http://www.sciencedirect.com/science/article/pii/S2215017X19304473
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