Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
Ribotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of...
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Instituto Oswaldo Cruz, Ministério da Saúde
2007-08-01
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doaj-cc3aceb215214527bffec9178610b77f2020-11-24T20:46:00ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602007-08-011025587592Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in BrazilCarlos Henrique Gomes MartinsTaís Maria BauabClarice Queico Fujimura LeiteDeise Pasetto FalcãoRibotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000500008ribotypingYersinia pseudotuberculosisvirulence markerspolymerase chain reaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carlos Henrique Gomes Martins Taís Maria Bauab Clarice Queico Fujimura Leite Deise Pasetto Falcão |
spellingShingle |
Carlos Henrique Gomes Martins Taís Maria Bauab Clarice Queico Fujimura Leite Deise Pasetto Falcão Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil Memórias do Instituto Oswaldo Cruz. ribotyping Yersinia pseudotuberculosis virulence markers polymerase chain reaction |
author_facet |
Carlos Henrique Gomes Martins Taís Maria Bauab Clarice Queico Fujimura Leite Deise Pasetto Falcão |
author_sort |
Carlos Henrique Gomes Martins |
title |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_short |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_full |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_fullStr |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_full_unstemmed |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_sort |
ribotyping and virulence markers of yersinia pseudotuberculosis strains isolated from animals in brazil |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2007-08-01 |
description |
Ribotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds. |
topic |
ribotyping Yersinia pseudotuberculosis virulence markers polymerase chain reaction |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000500008 |
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