Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes
Abstract Background Tsetse flies (Glossina sp.) are the vectors of human and animal trypanosomiasis throughout sub-Saharan Africa. Tsetse flies are distinguished from other Diptera by unique adaptations, including lactation and the birthing of live young (obligate viviparity), a vertebrate blood-spe...
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2019-09-01
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Series: | Genome Biology |
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Online Access: | http://link.springer.com/article/10.1186/s13059-019-1768-2 |
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English |
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author |
Geoffrey M. Attardo Adly M. M. Abd-Alla Alvaro Acosta-Serrano James E. Allen Rosemary Bateta Joshua B. Benoit Kostas Bourtzis Jelle Caers Guy Caljon Mikkel B. Christensen David W. Farrow Markus Friedrich Aurélie Hua-Van Emily C. Jennings Denis M. Larkin Daniel Lawson Michael J. Lehane Vasileios P. Lenis Ernesto Lowy-Gallego Rosaline W. Macharia Anna R. Malacrida Heather G. Marco Daniel Masiga Gareth L. Maslen Irina Matetovici Richard P. Meisel Irene Meki Veronika Michalkova Wolfgang J. Miller Patrick Minx Paul O. Mireji Lino Ometto Andrew G. Parker Rita Rio Clair Rose Andrew J. Rosendale Omar Rota-Stabelli Grazia Savini Liliane Schoofs Francesca Scolari Martin T. Swain Peter Takáč Chad Tomlinson George Tsiamis Jan Van Den Abbeele Aurelien Vigneron Jingwen Wang Wesley C. Warren Robert M. Waterhouse Matthew T. Weirauch Brian L. Weiss Richard K. Wilson Xin Zhao Serap Aksoy |
spellingShingle |
Geoffrey M. Attardo Adly M. M. Abd-Alla Alvaro Acosta-Serrano James E. Allen Rosemary Bateta Joshua B. Benoit Kostas Bourtzis Jelle Caers Guy Caljon Mikkel B. Christensen David W. Farrow Markus Friedrich Aurélie Hua-Van Emily C. Jennings Denis M. Larkin Daniel Lawson Michael J. Lehane Vasileios P. Lenis Ernesto Lowy-Gallego Rosaline W. Macharia Anna R. Malacrida Heather G. Marco Daniel Masiga Gareth L. Maslen Irina Matetovici Richard P. Meisel Irene Meki Veronika Michalkova Wolfgang J. Miller Patrick Minx Paul O. Mireji Lino Ometto Andrew G. Parker Rita Rio Clair Rose Andrew J. Rosendale Omar Rota-Stabelli Grazia Savini Liliane Schoofs Francesca Scolari Martin T. Swain Peter Takáč Chad Tomlinson George Tsiamis Jan Van Den Abbeele Aurelien Vigneron Jingwen Wang Wesley C. Warren Robert M. Waterhouse Matthew T. Weirauch Brian L. Weiss Richard K. Wilson Xin Zhao Serap Aksoy Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes Genome Biology Tsetse Trypanosomiasis Hematophagy Lactation Disease Neglected |
author_facet |
Geoffrey M. Attardo Adly M. M. Abd-Alla Alvaro Acosta-Serrano James E. Allen Rosemary Bateta Joshua B. Benoit Kostas Bourtzis Jelle Caers Guy Caljon Mikkel B. Christensen David W. Farrow Markus Friedrich Aurélie Hua-Van Emily C. Jennings Denis M. Larkin Daniel Lawson Michael J. Lehane Vasileios P. Lenis Ernesto Lowy-Gallego Rosaline W. Macharia Anna R. Malacrida Heather G. Marco Daniel Masiga Gareth L. Maslen Irina Matetovici Richard P. Meisel Irene Meki Veronika Michalkova Wolfgang J. Miller Patrick Minx Paul O. Mireji Lino Ometto Andrew G. Parker Rita Rio Clair Rose Andrew J. Rosendale Omar Rota-Stabelli Grazia Savini Liliane Schoofs Francesca Scolari Martin T. Swain Peter Takáč Chad Tomlinson George Tsiamis Jan Van Den Abbeele Aurelien Vigneron Jingwen Wang Wesley C. Warren Robert M. Waterhouse Matthew T. Weirauch Brian L. Weiss Richard K. Wilson Xin Zhao Serap Aksoy |
author_sort |
Geoffrey M. Attardo |
title |
Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes |
title_short |
Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes |
title_full |
Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes |
title_fullStr |
Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes |
title_full_unstemmed |
Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes |
title_sort |
comparative genomic analysis of six glossina genomes, vectors of african trypanosomes |
publisher |
BMC |
series |
Genome Biology |
issn |
1474-760X |
publishDate |
2019-09-01 |
description |
Abstract Background Tsetse flies (Glossina sp.) are the vectors of human and animal trypanosomiasis throughout sub-Saharan Africa. Tsetse flies are distinguished from other Diptera by unique adaptations, including lactation and the birthing of live young (obligate viviparity), a vertebrate blood-specific diet by both sexes, and obligate bacterial symbiosis. This work describes the comparative analysis of six Glossina genomes representing three sub-genera: Morsitans (G. morsitans morsitans, G. pallidipes, G. austeni), Palpalis (G. palpalis, G. fuscipes), and Fusca (G. brevipalpis) which represent different habitats, host preferences, and vectorial capacity. Results Genomic analyses validate established evolutionary relationships and sub-genera. Syntenic analysis of Glossina relative to Drosophila melanogaster shows reduced structural conservation across the sex-linked X chromosome. Sex-linked scaffolds show increased rates of female-specific gene expression and lower evolutionary rates relative to autosome associated genes. Tsetse-specific genes are enriched in protease, odorant-binding, and helicase activities. Lactation-associated genes are conserved across all Glossina species while male seminal proteins are rapidly evolving. Olfactory and gustatory genes are reduced across the genus relative to other insects. Vision-associated Rhodopsin genes show conservation of motion detection/tracking functions and variance in the Rhodopsin detecting colors in the blue wavelength ranges. Conclusions Expanded genomic discoveries reveal the genetics underlying Glossina biology and provide a rich body of knowledge for basic science and disease control. They also provide insight into the evolutionary biology underlying novel adaptations and are relevant to applied aspects of vector control such as trap design and discovery of novel pest and disease control strategies. |
topic |
Tsetse Trypanosomiasis Hematophagy Lactation Disease Neglected |
url |
http://link.springer.com/article/10.1186/s13059-019-1768-2 |
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doaj-cc3ed49129c9474f93f589276ff7bcfe2020-11-25T03:44:59ZengBMCGenome Biology1474-760X2019-09-0120113110.1186/s13059-019-1768-2Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomesGeoffrey M. Attardo0Adly M. M. Abd-Alla1Alvaro Acosta-Serrano2James E. Allen3Rosemary Bateta4Joshua B. Benoit5Kostas Bourtzis6Jelle Caers7Guy Caljon8Mikkel B. Christensen9David W. Farrow10Markus Friedrich11Aurélie Hua-Van12Emily C. Jennings13Denis M. Larkin14Daniel Lawson15Michael J. Lehane16Vasileios P. Lenis17Ernesto Lowy-Gallego18Rosaline W. Macharia19Anna R. Malacrida20Heather G. Marco21Daniel Masiga22Gareth L. Maslen23Irina Matetovici24Richard P. Meisel25Irene Meki26Veronika Michalkova27Wolfgang J. Miller28Patrick Minx29Paul O. Mireji30Lino Ometto31Andrew G. Parker32Rita Rio33Clair Rose34Andrew J. Rosendale35Omar Rota-Stabelli36Grazia Savini37Liliane Schoofs38Francesca Scolari39Martin T. Swain40Peter Takáč41Chad Tomlinson42George Tsiamis43Jan Van Den Abbeele44Aurelien Vigneron45Jingwen Wang46Wesley C. Warren47Robert M. Waterhouse48Matthew T. Weirauch49Brian L. Weiss50Richard K. Wilson51Xin Zhao52Serap Aksoy53Department of Entomology and Nematology, University of California, DavisInsect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food & AgricultureDepartment of Vector Biology, Liverpool School of Tropical MedicineVectorBase, European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)Department of Biochemistry, Biotechnology Research Institute - Kenya Agricultural and Livestock Research OrganizationDepartment of Biological Sciences, University of CincinnatiInsect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food & AgricultureDepartment of Biology - Functional Genomics and Proteomics Group, KU LeuvenLaboratory of Microbiology, Parasitology and Hygiene, University of AntwerpVectorBase, European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)Department of Biological Sciences, University of CincinnatiDepartment of Biological Sciences, Wayne State UniversityLaboratoire Evolution, Genomes, Comportement, Ecologie, CNRS, IRD, Univ. Paris-Sud, Université Paris-SaclayDepartment of Biological Sciences, University of CincinnatiDepartment of Comparative Biomedical Sciences, Royal Veterinary CollegeDepartment of Life Sciences, Imperial College LondonDepartment of Vector Biology, Liverpool School of Tropical MedicineSchools of Medicine and Dentistry, University of PlymouthVectorBase, European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)Molecular Biology and Bioinformatics Unit, International Center for Insect Physiology and EcologyDepartment of Biology and Biotechnology, University of PaviaDepartment of Biological Sciences, University of Cape TownMolecular Biology and Bioinformatics Unit, International Center for Insect Physiology and EcologyVectorBase, European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)Biomedical Sciences, Institute of Tropical MedicineDepartment of Biology and Biochemistry, University of HoustonInsect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food & AgricultureDepartment of Biological Sciences, Florida International UniversityDepartment of Cell and Developmental Biology, Medical University of ViennaMcDonnell Genome Institute, Washington University School of MedicineDepartment of Biochemistry, Biotechnology Research Institute - Kenya Agricultural and Livestock Research OrganizationDepartment of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund MachInsect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food & AgricultureDepartment of Biology, West Virginia UniversityDepartment of Vector Biology, Liverpool School of Tropical MedicineDepartment of Biology, Mount St. Joseph UniversityDepartment of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund MachDepartment of Biology and Biotechnology, University of PaviaDepartment of Biology - Functional Genomics and Proteomics Group, KU LeuvenDepartment of Biology and Biotechnology, University of PaviaInstitute of Biological, Environmental and Rural Sciences, Aberystwyth UniversityDepartment of Animal Systematics, Ústav zoológie SAV; Scientica, LtdMcDonnell Genome Institute, Washington University School of MedicineDepartment of Environmental and Natural Resources Management, University of PatrasBiomedical Sciences, Institute of Tropical MedicineDepartment of Epidemiology of Microbial Diseases, Yale School of Public HealthSchool of Life Sciences, Fudan UniversityMcDonnell Genome Institute, Washington University School of MedicineDepartment of Ecology & Evolution, Swiss Institute of Bioinformatics, University of LausanneCenter for Autoimmune Genomics and Etiology and Divisions of Biomedical Informatics and Developmental Biology, Cincinnati Children’s Hospital Medical CenterDepartment of Epidemiology of Microbial Diseases, Yale School of Public HealthMcDonnell Genome Institute, Washington University School of MedicineCAS Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of SciencesDepartment of Epidemiology of Microbial Diseases, Yale School of Public HealthAbstract Background Tsetse flies (Glossina sp.) are the vectors of human and animal trypanosomiasis throughout sub-Saharan Africa. Tsetse flies are distinguished from other Diptera by unique adaptations, including lactation and the birthing of live young (obligate viviparity), a vertebrate blood-specific diet by both sexes, and obligate bacterial symbiosis. This work describes the comparative analysis of six Glossina genomes representing three sub-genera: Morsitans (G. morsitans morsitans, G. pallidipes, G. austeni), Palpalis (G. palpalis, G. fuscipes), and Fusca (G. brevipalpis) which represent different habitats, host preferences, and vectorial capacity. Results Genomic analyses validate established evolutionary relationships and sub-genera. Syntenic analysis of Glossina relative to Drosophila melanogaster shows reduced structural conservation across the sex-linked X chromosome. Sex-linked scaffolds show increased rates of female-specific gene expression and lower evolutionary rates relative to autosome associated genes. Tsetse-specific genes are enriched in protease, odorant-binding, and helicase activities. Lactation-associated genes are conserved across all Glossina species while male seminal proteins are rapidly evolving. Olfactory and gustatory genes are reduced across the genus relative to other insects. Vision-associated Rhodopsin genes show conservation of motion detection/tracking functions and variance in the Rhodopsin detecting colors in the blue wavelength ranges. Conclusions Expanded genomic discoveries reveal the genetics underlying Glossina biology and provide a rich body of knowledge for basic science and disease control. They also provide insight into the evolutionary biology underlying novel adaptations and are relevant to applied aspects of vector control such as trap design and discovery of novel pest and disease control strategies.http://link.springer.com/article/10.1186/s13059-019-1768-2TsetseTrypanosomiasisHematophagyLactationDiseaseNeglected |