Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic Patients
Asthma is a common airway inflammation with an intricate underlying mechanism. The role played by circulating miRNAs in asthma remains unclear. In the present study, we aimed to investigate the role of miR-223-3p in leukocytes of asthma and identify the relationship between miR-223-3p and inflammat...
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Tehran University of Medical Sciences
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doaj-cc65acddc7fa4e1ca9459d04fd4223ae2020-11-25T04:08:44ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492020-06-0119310.18502/ijaai.v19i3.34562497Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic PatientsWenjuan Xu0Yimin Wang1Chen Wang2Ying Ma3Shaojun He4Yanmeng Kang5Jiong Yang6Department of Respiratory and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Respiratory and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaClinical Laboratory, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Respiratory and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Respiratory and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of Respiratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, ChinaDepartment of Respiratory and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China Asthma is a common airway inflammation with an intricate underlying mechanism. The role played by circulating miRNAs in asthma remains unclear. In the present study, we aimed to investigate the role of miR-223-3p in leukocytes of asthma and identify the relationship between miR-223-3p and inflammatory cytokines in asthma. Using real-time polymerase chain reaction (RT-PCR), we detected miR-223-3p expression in peripheral blood leukocytes from 23 asthmatic patients and 20 healthy controls. The levels of IFN-γ (Th1 cytokine), IL-4 (Th2 cytokine), IL-17A (Th17 cytokine) in plasma were examined using enzyme-linked immunosorbent assay (ELISA). Analysis of variance (ANOVA) and Spearman’s test was used for statistical analysis. The expression of miR-223-3p in peripheral blood leukocytes was upregulated in the asthmatic patients compared with that in the healthy controls. Increased miR-223-3p expression was associated with forced expiratory volume in 1-second percent predicted (FEV1% predicted). A positive correlation was noted between miR-223-3p and IL-17A. The findings of this study showed that miR-223-3p plays a vital role in the pathogenesis of asthma and can serve as a novel biomarker for asthma. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2497AsthmaInterleukin-17miR-223-3p |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenjuan Xu Yimin Wang Chen Wang Ying Ma Shaojun He Yanmeng Kang Jiong Yang |
spellingShingle |
Wenjuan Xu Yimin Wang Chen Wang Ying Ma Shaojun He Yanmeng Kang Jiong Yang Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic Patients Iranian Journal of Allergy, Asthma and Immunology Asthma Interleukin-17 miR-223-3p |
author_facet |
Wenjuan Xu Yimin Wang Chen Wang Ying Ma Shaojun He Yanmeng Kang Jiong Yang |
author_sort |
Wenjuan Xu |
title |
Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic Patients |
title_short |
Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic Patients |
title_full |
Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic Patients |
title_fullStr |
Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic Patients |
title_full_unstemmed |
Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic Patients |
title_sort |
increased mir-223-3p in leukocytes positively correlated with il-17a in plasma of asthmatic patients |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Allergy, Asthma and Immunology |
issn |
1735-1502 1735-5249 |
publishDate |
2020-06-01 |
description |
Asthma is a common airway inflammation with an intricate underlying mechanism. The role played by circulating miRNAs in asthma remains unclear. In the present study, we aimed to investigate the role of miR-223-3p in leukocytes of asthma and identify the relationship between miR-223-3p and inflammatory cytokines in asthma.
Using real-time polymerase chain reaction (RT-PCR), we detected miR-223-3p expression in peripheral blood leukocytes from 23 asthmatic patients and 20 healthy controls. The levels of IFN-γ (Th1 cytokine), IL-4 (Th2 cytokine), IL-17A (Th17 cytokine) in plasma were examined using enzyme-linked immunosorbent assay (ELISA). Analysis of variance (ANOVA) and Spearman’s test was used for statistical analysis.
The expression of miR-223-3p in peripheral blood leukocytes was upregulated in the asthmatic patients compared with that in the healthy controls. Increased miR-223-3p expression was associated with forced expiratory volume in 1-second percent predicted (FEV1% predicted). A positive correlation was noted between miR-223-3p and IL-17A.
The findings of this study showed that miR-223-3p plays a vital role in the pathogenesis of asthma and can serve as a novel biomarker for asthma.
|
topic |
Asthma Interleukin-17 miR-223-3p |
url |
https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2497 |
work_keys_str_mv |
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