Zika Virus Replicons for Drug Discovery
The current epidemic of Zika virus (ZIKV) has underscored the urgency to establish experimental systems for studying viral replication and pathogenesis, and countermeasure development. Here we report two ZIKV replicon systems: a luciferase replicon that can differentiate between viral translation an...
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doaj-cc71a3d9730b4148bec5309eb12bdc032020-11-25T00:07:11ZengElsevierEBioMedicine2352-39642016-10-0112C15616010.1016/j.ebiom.2016.09.013Zika Virus Replicons for Drug DiscoveryXuping Xie0Jing Zou1Chao Shan2Yujiao Yang3Dieudonné Buh Kum4Kai Dallmeier5Johan Neyts6Pei-Yong Shi7Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USADepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USADepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USADepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USADepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USARega Institute for Medical Research, Laboratory of Virology and Chemotherapy, University of Leuven, Leuven, BelgiumRega Institute for Medical Research, Laboratory of Virology and Chemotherapy, University of Leuven, Leuven, BelgiumDepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USAThe current epidemic of Zika virus (ZIKV) has underscored the urgency to establish experimental systems for studying viral replication and pathogenesis, and countermeasure development. Here we report two ZIKV replicon systems: a luciferase replicon that can differentiate between viral translation and RNA synthesis; and a stable luciferase replicon carrying cell line that can be used to screen and characterize inhibitors of viral replication. The transient replicon was used to evaluate the effect of an NS5 polymerase mutation on viral RNA synthesis and to analyze a known ZIKV inhibitor. The replicon cell line was developed into a 96-well format for antiviral testing. Compare with virus infection-based assay, the replicon cell line allows antiviral screening without using infectious virus. Collectively, the replicon systems have provided critical tools for both basic and translational research.http://www.sciencedirect.com/science/article/pii/S2352396416304200ZikaDrug discoveryRepliconFlavivirus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xuping Xie Jing Zou Chao Shan Yujiao Yang Dieudonné Buh Kum Kai Dallmeier Johan Neyts Pei-Yong Shi |
spellingShingle |
Xuping Xie Jing Zou Chao Shan Yujiao Yang Dieudonné Buh Kum Kai Dallmeier Johan Neyts Pei-Yong Shi Zika Virus Replicons for Drug Discovery EBioMedicine Zika Drug discovery Replicon Flavivirus |
author_facet |
Xuping Xie Jing Zou Chao Shan Yujiao Yang Dieudonné Buh Kum Kai Dallmeier Johan Neyts Pei-Yong Shi |
author_sort |
Xuping Xie |
title |
Zika Virus Replicons for Drug Discovery |
title_short |
Zika Virus Replicons for Drug Discovery |
title_full |
Zika Virus Replicons for Drug Discovery |
title_fullStr |
Zika Virus Replicons for Drug Discovery |
title_full_unstemmed |
Zika Virus Replicons for Drug Discovery |
title_sort |
zika virus replicons for drug discovery |
publisher |
Elsevier |
series |
EBioMedicine |
issn |
2352-3964 |
publishDate |
2016-10-01 |
description |
The current epidemic of Zika virus (ZIKV) has underscored the urgency to establish experimental systems for studying viral replication and pathogenesis, and countermeasure development. Here we report two ZIKV replicon systems: a luciferase replicon that can differentiate between viral translation and RNA synthesis; and a stable luciferase replicon carrying cell line that can be used to screen and characterize inhibitors of viral replication. The transient replicon was used to evaluate the effect of an NS5 polymerase mutation on viral RNA synthesis and to analyze a known ZIKV inhibitor. The replicon cell line was developed into a 96-well format for antiviral testing. Compare with virus infection-based assay, the replicon cell line allows antiviral screening without using infectious virus. Collectively, the replicon systems have provided critical tools for both basic and translational research. |
topic |
Zika Drug discovery Replicon Flavivirus |
url |
http://www.sciencedirect.com/science/article/pii/S2352396416304200 |
work_keys_str_mv |
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