The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity
Obesity and hyperlipidemia are a major problem in the world. Pluchea indica (L.) Less. tea (PIT) is a beverage that has various indications. This study focused on the effect of the PIT on inhibiting adipogenesis of 3T3-L1 cells and pancreatic lipase enzyme activity. The viability of 3T3-L1 cells was...
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doaj-cc78e422c8794849a3a44ff4e5f7e4152020-11-24T23:15:07ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882018-01-01201810.1155/2018/41087874108787The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase ActivityKittipot Sirichaiwetchakoon0Gordon Matthew Lowe1Kanjana Thumanu2Griangsak Eumkeb3School of Preclinic, Institute of Science, Suranaree University of Technology, 111 University Avenue, Suranaree Subdistrict, Muang District, Nakhonratchasima 30000, ThailandSchool of Pharmacy and Biomolecular Sciences, Liverpool John Moores Univerisity, James Parsons Building, Byrom Street, Liverpool, UKSynchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, 30000, ThailandSchool of Preclinic, Institute of Science, Suranaree University of Technology, 111 University Avenue, Suranaree Subdistrict, Muang District, Nakhonratchasima 30000, ThailandObesity and hyperlipidemia are a major problem in the world. Pluchea indica (L.) Less. tea (PIT) is a beverage that has various indications. This study focused on the effect of the PIT on inhibiting adipogenesis of 3T3-L1 cells and pancreatic lipase enzyme activity. The viability of 3T3-L1 cells was not significantly decreased after exposure to 200 to 1000 μg mL−1 PIT compared to controls (p>0.05). The PIT at 750 to 1000 μg mL−1 exhibited a significantly reduced lipid accumulation compared to the control (p<0.05). The inhibitory effects of the PIT at 250 to 1000 μg mL−1 on lipase activity were significantly increased compared to control (p<0.05). The FTIR results showed that the integrated areas of lipids, proteins, nucleic acids, glycogen, and carbohydrates of the PIT-treated 3T3-L1 adipocytes were significantly lower than the untreated 3T3-L1 adipocytes (p<0.05). These findings may indicate that the PIT is not only capable of inhibiting lipids and carbohydrate accumulation in adipocytes but also has a potential to inhibit pancreatic lipase activity. So, the PIT may be further developed to the novel lipid-lowering herbal supplement for the management of overweight or obesity.http://dx.doi.org/10.1155/2018/4108787 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kittipot Sirichaiwetchakoon Gordon Matthew Lowe Kanjana Thumanu Griangsak Eumkeb |
spellingShingle |
Kittipot Sirichaiwetchakoon Gordon Matthew Lowe Kanjana Thumanu Griangsak Eumkeb The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity Evidence-Based Complementary and Alternative Medicine |
author_facet |
Kittipot Sirichaiwetchakoon Gordon Matthew Lowe Kanjana Thumanu Griangsak Eumkeb |
author_sort |
Kittipot Sirichaiwetchakoon |
title |
The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity |
title_short |
The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity |
title_full |
The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity |
title_fullStr |
The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity |
title_full_unstemmed |
The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity |
title_sort |
effect of pluchea indica (l.) less. tea on adipogenesis in 3t3-l1 adipocytes and lipase activity |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2018-01-01 |
description |
Obesity and hyperlipidemia are a major problem in the world. Pluchea indica (L.) Less. tea (PIT) is a beverage that has various indications. This study focused on the effect of the PIT on inhibiting adipogenesis of 3T3-L1 cells and pancreatic lipase enzyme activity. The viability of 3T3-L1 cells was not significantly decreased after exposure to 200 to 1000 μg mL−1 PIT compared to controls (p>0.05). The PIT at 750 to 1000 μg mL−1 exhibited a significantly reduced lipid accumulation compared to the control (p<0.05). The inhibitory effects of the PIT at 250 to 1000 μg mL−1 on lipase activity were significantly increased compared to control (p<0.05). The FTIR results showed that the integrated areas of lipids, proteins, nucleic acids, glycogen, and carbohydrates of the PIT-treated 3T3-L1 adipocytes were significantly lower than the untreated 3T3-L1 adipocytes (p<0.05). These findings may indicate that the PIT is not only capable of inhibiting lipids and carbohydrate accumulation in adipocytes but also has a potential to inhibit pancreatic lipase activity. So, the PIT may be further developed to the novel lipid-lowering herbal supplement for the management of overweight or obesity. |
url |
http://dx.doi.org/10.1155/2018/4108787 |
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