Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi
Introduction: Molecular biology techniques have allowed a better knowledge of sources of blood meals in vector insects. However, the usefulness of these techniques depends on both the quantity of ingested blood and the digestion process in the insect. Objective: To identify the time limit for detect...
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doaj-cc7c20efead84c898bc5fa5dde59b0e42020-11-24T22:25:24ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572017-03-0137018719210.7705/biomedica.v37i0.33962057Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansiJosé Gabriel Vergara0Daniel Verbel-Vergara1Ana Milena Montesino2Alveiro Pérez-Doria3Eduar Elías Bejarano4Grupo de Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, ColombiaGrupo de Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, ColombiaGrupo de Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, ColombiaGrupo de Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, ColombiaGrupo de Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, ColombiaIntroduction: Molecular biology techniques have allowed a better knowledge of sources of blood meals in vector insects. However, the usefulness of these techniques depends on both the quantity of ingested blood and the digestion process in the insect. Objective: To identify the time limit for detection of the human cytochrome b (Cyt b) gene in experimentally fed females of Lutzomyia evansi. Materials and methods: Eight groups of L. evansi females were fed on human blood and sacrificed at intervals of 24 hours post-ingestion. Total DNA was extracted from each female and a segment of 358 bp of Cyt b was amplified. In order to eliminate false positives, amplification products were subjected to a restriction fragment length polymorphism (RFLP) analysis. Results: The human Cyt b gene segment was detected in 86% (49/57) of the females of L. evansi, from 0 to 168 hours after blood ingestion. In 7% (4/57) of the individuals we amplified insect DNA, while in the remaining 7%, the band of interest was not amplified. We did not find any statistical differences between groups of females sacrificed at different times post-blood meal regarding the amplification of the human Cyt b gene segment or the number of samples amplified. Conclusion: The human Cyt b gene segment was detectable in L. evansi females up to 168 hours after blood ingestion.https://www.revistabiomedica.org/index.php/biomedica/article/view/3396Psychodidaecytochrome binsect vectorsbloodleishmaniasis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
José Gabriel Vergara Daniel Verbel-Vergara Ana Milena Montesino Alveiro Pérez-Doria Eduar Elías Bejarano |
spellingShingle |
José Gabriel Vergara Daniel Verbel-Vergara Ana Milena Montesino Alveiro Pérez-Doria Eduar Elías Bejarano Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi Biomédica: revista del Instituto Nacional de Salud Psychodidae cytochrome b insect vectors blood leishmaniasis |
author_facet |
José Gabriel Vergara Daniel Verbel-Vergara Ana Milena Montesino Alveiro Pérez-Doria Eduar Elías Bejarano |
author_sort |
José Gabriel Vergara |
title |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_short |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_full |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_fullStr |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_full_unstemmed |
Estimation of time detection limit for human cytochrome b in females of Lutzomyia evansi |
title_sort |
estimation of time detection limit for human cytochrome b in females of lutzomyia evansi |
publisher |
Instituto Nacional de Salud |
series |
Biomédica: revista del Instituto Nacional de Salud |
issn |
0120-4157 0120-4157 |
publishDate |
2017-03-01 |
description |
Introduction: Molecular biology techniques have allowed a better knowledge of sources of blood meals in vector insects. However, the usefulness of these techniques depends on both the quantity of ingested blood and the digestion process in the insect.
Objective: To identify the time limit for detection of the human cytochrome b (Cyt b) gene in experimentally fed females of Lutzomyia evansi.
Materials and methods: Eight groups of L. evansi females were fed on human blood and sacrificed at intervals of 24 hours post-ingestion. Total DNA was extracted from each female and a segment of 358 bp of Cyt b was amplified. In order to eliminate false positives, amplification products were subjected to a restriction fragment length polymorphism (RFLP) analysis.
Results: The human Cyt b gene segment was detected in 86% (49/57) of the females of L. evansi, from 0 to 168 hours after blood ingestion. In 7% (4/57) of the individuals we amplified insect DNA, while in the remaining 7%, the band of interest was not amplified. We did not find any statistical differences between groups of females sacrificed at different times post-blood meal regarding the amplification of the human Cyt b gene segment or the number of samples amplified.
Conclusion: The human Cyt b gene segment was detectable in L. evansi females up to 168 hours after blood ingestion. |
topic |
Psychodidae cytochrome b insect vectors blood leishmaniasis |
url |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3396 |
work_keys_str_mv |
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