Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala)
Blunt snout bream (Megalobrama amblycephala) is a widely favored herbivorous fish species and is a frequentlyused fish model for studying the metabolism physiology. This study aimed to provide a comprehensive illustration of the mechanisms of a high-starch diet (HSD) induced lipid metabolic disorder...
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doaj-cc83e504500d4a27a5513bf122cbdbc82020-11-24T23:59:40ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-05-01186116110.3390/ijms18061161ijms18061161Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala)Ling-Hong Miao0Yan Lin1Wen-Jing Pan2Xin Huang3Xian-Ping Ge4Ming-Chun Ren5Qun-Lan Zhou6Bo Liu7Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaBlunt snout bream (Megalobrama amblycephala) is a widely favored herbivorous fish species and is a frequentlyused fish model for studying the metabolism physiology. This study aimed to provide a comprehensive illustration of the mechanisms of a high-starch diet (HSD) induced lipid metabolic disorder by identifying microRNAs (miRNAs) controlled pathways in glucose and lipid metabolism in fish using high-throughput sequencing technologies. Small RNA libraries derived from intestines, livers, and brains of HSD and normal-starch diet (NSD) treated M. amblycephala were sequenced and 79, 124 and 77 differentially expressed miRNAs (DEMs) in intestines, livers, and brains of HSD treated fish were identified, respectively. Bioinformatics analyses showed that these DEMs targeted hundreds of predicted genes were enriched into metabolic pathways and biosynthetic processes, including peroxisome proliferator-activated receptor (PPAR), glycolysis/gluconeogenesis, and insulin signaling pathway. These analyses confirmed that miRNAs play crucial roles in glucose and lipid metabolism related to high wheat starch treatment. These results provide information on further investigation of a DEM-related mechanism dysregulated by a high carbohydrate diet.http://www.mdpi.com/1422-0067/18/6/1161microRNAswheat starchglucose and lipid metabolismMegalobrama amblycephala |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ling-Hong Miao Yan Lin Wen-Jing Pan Xin Huang Xian-Ping Ge Ming-Chun Ren Qun-Lan Zhou Bo Liu |
spellingShingle |
Ling-Hong Miao Yan Lin Wen-Jing Pan Xin Huang Xian-Ping Ge Ming-Chun Ren Qun-Lan Zhou Bo Liu Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala) International Journal of Molecular Sciences microRNAs wheat starch glucose and lipid metabolism Megalobrama amblycephala |
author_facet |
Ling-Hong Miao Yan Lin Wen-Jing Pan Xin Huang Xian-Ping Ge Ming-Chun Ren Qun-Lan Zhou Bo Liu |
author_sort |
Ling-Hong Miao |
title |
Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala) |
title_short |
Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala) |
title_full |
Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala) |
title_fullStr |
Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala) |
title_full_unstemmed |
Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala) |
title_sort |
identification of differentially expressed micrornas associate with glucose metabolism in different organs of blunt snout bream (megalobrama amblycephala) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-05-01 |
description |
Blunt snout bream (Megalobrama amblycephala) is a widely favored herbivorous fish species and is a frequentlyused fish model for studying the metabolism physiology. This study aimed to provide a comprehensive illustration of the mechanisms of a high-starch diet (HSD) induced lipid metabolic disorder by identifying microRNAs (miRNAs) controlled pathways in glucose and lipid metabolism in fish using high-throughput sequencing technologies. Small RNA libraries derived from intestines, livers, and brains of HSD and normal-starch diet (NSD) treated M. amblycephala were sequenced and 79, 124 and 77 differentially expressed miRNAs (DEMs) in intestines, livers, and brains of HSD treated fish were identified, respectively. Bioinformatics analyses showed that these DEMs targeted hundreds of predicted genes were enriched into metabolic pathways and biosynthetic processes, including peroxisome proliferator-activated receptor (PPAR), glycolysis/gluconeogenesis, and insulin signaling pathway. These analyses confirmed that miRNAs play crucial roles in glucose and lipid metabolism related to high wheat starch treatment. These results provide information on further investigation of a DEM-related mechanism dysregulated by a high carbohydrate diet. |
topic |
microRNAs wheat starch glucose and lipid metabolism Megalobrama amblycephala |
url |
http://www.mdpi.com/1422-0067/18/6/1161 |
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