Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies

Polyoxometalates (POMs) have become very significant in biomedical research for their structural diversity which renders them highly active against bacterial, viral and cancer diseases. In this study three different POMs were synthesized and nanoassemblies were made with chitosan (CTS), a natural bi...

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Main Authors: Hamid Saeed Shah, Rami Al-Oweini, Ali Haider, Ulrich Kortz, Jamshed Iqbal
Format: Article
Language:English
Published: Elsevier 2014-01-01
Series:Toxicology Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2214750014000341
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spelling doaj-cc84aaf477f34860bc7812e44e1d40cf2020-11-25T00:45:19ZengElsevierToxicology Reports2214-75002014-01-011C34135210.1016/j.toxrep.2014.06.001Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassembliesHamid Saeed Shah0Rami Al-Oweini1Ali Haider2Ulrich Kortz3Jamshed Iqbal4Centre for Advanced Drug Research, COMSATS Institute of Information Technology, Abbottabad 22060, PakistanSchool of Engineering and Science, Jacobs University, P.O. Box 750 561, 28725 Bremen, GermanySchool of Engineering and Science, Jacobs University, P.O. Box 750 561, 28725 Bremen, GermanySchool of Engineering and Science, Jacobs University, P.O. Box 750 561, 28725 Bremen, GermanyCentre for Advanced Drug Research, COMSATS Institute of Information Technology, Abbottabad 22060, PakistanPolyoxometalates (POMs) have become very significant in biomedical research for their structural diversity which renders them highly active against bacterial, viral and cancer diseases. In this study three different POMs were synthesized and nanoassemblies were made with chitosan (CTS), a natural biodegradable polymer with excellent drug carrier properties. The compounds were tested on two isoenzymes of alkaline phosphatases including tissue specific calf intestine alkaline phosphatase (CIAP) and tissue non-specific alkaline phosphatase (TNAP). Compound [TeW6O24]6− (TeW6) showed the highest activity (45.4 ± 11.3 nM) among tested compounds against TNAP. Similarly, chitosan-[TeW6O24]6− (CTS-TeW6) was proved to be a potent inhibitor of CIAP with Ki value of 22 ± 7 nM. A comparative study was made to evaluate their cytotoxic potential against HeLa cells. Among all tested compounds, Chitosan-[NaP5W30O110]14− (CTS-P5W30) has showed higher percent cytotoxicity (88 ± 10%) at 10 μM when compared with the standard anticancer drug vincristine (72 ± 7%). The study revealed that selected POMs proved excellent anticancer potential and were equally effective against alkaline phosphatase enzyme, an increased level of which may indicate cancer metastasis.http://www.sciencedirect.com/science/article/pii/S2214750014000341PolyoxometalateChitosanCytotoxicityAlkaline phosphataseChitosan-polyoxometalate nanoassemblies
collection DOAJ
language English
format Article
sources DOAJ
author Hamid Saeed Shah
Rami Al-Oweini
Ali Haider
Ulrich Kortz
Jamshed Iqbal
spellingShingle Hamid Saeed Shah
Rami Al-Oweini
Ali Haider
Ulrich Kortz
Jamshed Iqbal
Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies
Toxicology Reports
Polyoxometalate
Chitosan
Cytotoxicity
Alkaline phosphatase
Chitosan-polyoxometalate nanoassemblies
author_facet Hamid Saeed Shah
Rami Al-Oweini
Ali Haider
Ulrich Kortz
Jamshed Iqbal
author_sort Hamid Saeed Shah
title Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies
title_short Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies
title_full Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies
title_fullStr Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies
title_full_unstemmed Cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies
title_sort cytotoxicity and enzyme inhibition studies of polyoxometalates and their chitosan nanoassemblies
publisher Elsevier
series Toxicology Reports
issn 2214-7500
publishDate 2014-01-01
description Polyoxometalates (POMs) have become very significant in biomedical research for their structural diversity which renders them highly active against bacterial, viral and cancer diseases. In this study three different POMs were synthesized and nanoassemblies were made with chitosan (CTS), a natural biodegradable polymer with excellent drug carrier properties. The compounds were tested on two isoenzymes of alkaline phosphatases including tissue specific calf intestine alkaline phosphatase (CIAP) and tissue non-specific alkaline phosphatase (TNAP). Compound [TeW6O24]6− (TeW6) showed the highest activity (45.4 ± 11.3 nM) among tested compounds against TNAP. Similarly, chitosan-[TeW6O24]6− (CTS-TeW6) was proved to be a potent inhibitor of CIAP with Ki value of 22 ± 7 nM. A comparative study was made to evaluate their cytotoxic potential against HeLa cells. Among all tested compounds, Chitosan-[NaP5W30O110]14− (CTS-P5W30) has showed higher percent cytotoxicity (88 ± 10%) at 10 μM when compared with the standard anticancer drug vincristine (72 ± 7%). The study revealed that selected POMs proved excellent anticancer potential and were equally effective against alkaline phosphatase enzyme, an increased level of which may indicate cancer metastasis.
topic Polyoxometalate
Chitosan
Cytotoxicity
Alkaline phosphatase
Chitosan-polyoxometalate nanoassemblies
url http://www.sciencedirect.com/science/article/pii/S2214750014000341
work_keys_str_mv AT hamidsaeedshah cytotoxicityandenzymeinhibitionstudiesofpolyoxometalatesandtheirchitosannanoassemblies
AT ramialoweini cytotoxicityandenzymeinhibitionstudiesofpolyoxometalatesandtheirchitosannanoassemblies
AT alihaider cytotoxicityandenzymeinhibitionstudiesofpolyoxometalatesandtheirchitosannanoassemblies
AT ulrichkortz cytotoxicityandenzymeinhibitionstudiesofpolyoxometalatesandtheirchitosannanoassemblies
AT jamshediqbal cytotoxicityandenzymeinhibitionstudiesofpolyoxometalatesandtheirchitosannanoassemblies
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