In vitro and in vivo antitumor activity of a novel pH-activated polymeric drug delivery system for doxorubicin.
<h4>Background</h4>Conventional chemotherapy agent such as doxorubicin (DOX) is of limited clinical use because of its inherently low selectivity, which can lead to systemic toxicity in normal healthy tissue.<h4>Methods</h4>A pH stimuli-sensitive conjugate based on polyethyle...
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doaj-cc8936b69110404391661108caa659af2021-03-04T00:16:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4411610.1371/journal.pone.0044116In vitro and in vivo antitumor activity of a novel pH-activated polymeric drug delivery system for doxorubicin.Menglei HuanBangle ZhangZenghui TengHan CuiJieping WangXinyou LiuHui XiaSiyuan ZhouQibing Mei<h4>Background</h4>Conventional chemotherapy agent such as doxorubicin (DOX) is of limited clinical use because of its inherently low selectivity, which can lead to systemic toxicity in normal healthy tissue.<h4>Methods</h4>A pH stimuli-sensitive conjugate based on polyethylene glycol (PEG) with covalently attachment doxorubicin via hydrazone bond (PEG-hyd-DOX) was prepared for tumor targeting delivery system. While PEG-DOX conjugates via amid bond (PEG-ami-DOX) was synthesized as control.<h4>Results</h4>The synthetic conjugates were confirmed by proton nuclear magnetic resonance (NMR) spectroscopy, the release profile of DOX from PEG-hyd-DOX was acid-liable for the hydrazone linkage between DOX and PEG, led to different intracellular uptake route; intracellular accumulation of PEG-hyd-DOX was higher than PEG-ami-DOX due to its pH-triggered profile, and thereby more cytotoxicity against MCF-7, MDA-MB-231 (breast cancer models) and HepG2 (hepatocellular carcinoma model) cell lines. Following the in vitro results, we xenografted MDA-MB-231 cell onto SCID mice, PEG-hyd-DOX showed stronger antitumor efficacy than free DOX and was tumor-targeting.<h4>Conclusions</h4>Results from these in vivo experiments were consistent with our in vitro results; suggested this pH-triggered PEG-hyd-DOX conjugate could target DOX to tumor tissues and release free drugs by acidic tumor environment, which would be potent in antitumor drug delivery.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028490/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Menglei Huan Bangle Zhang Zenghui Teng Han Cui Jieping Wang Xinyou Liu Hui Xia Siyuan Zhou Qibing Mei |
spellingShingle |
Menglei Huan Bangle Zhang Zenghui Teng Han Cui Jieping Wang Xinyou Liu Hui Xia Siyuan Zhou Qibing Mei In vitro and in vivo antitumor activity of a novel pH-activated polymeric drug delivery system for doxorubicin. PLoS ONE |
author_facet |
Menglei Huan Bangle Zhang Zenghui Teng Han Cui Jieping Wang Xinyou Liu Hui Xia Siyuan Zhou Qibing Mei |
author_sort |
Menglei Huan |
title |
In vitro and in vivo antitumor activity of a novel pH-activated polymeric drug delivery system for doxorubicin. |
title_short |
In vitro and in vivo antitumor activity of a novel pH-activated polymeric drug delivery system for doxorubicin. |
title_full |
In vitro and in vivo antitumor activity of a novel pH-activated polymeric drug delivery system for doxorubicin. |
title_fullStr |
In vitro and in vivo antitumor activity of a novel pH-activated polymeric drug delivery system for doxorubicin. |
title_full_unstemmed |
In vitro and in vivo antitumor activity of a novel pH-activated polymeric drug delivery system for doxorubicin. |
title_sort |
in vitro and in vivo antitumor activity of a novel ph-activated polymeric drug delivery system for doxorubicin. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
<h4>Background</h4>Conventional chemotherapy agent such as doxorubicin (DOX) is of limited clinical use because of its inherently low selectivity, which can lead to systemic toxicity in normal healthy tissue.<h4>Methods</h4>A pH stimuli-sensitive conjugate based on polyethylene glycol (PEG) with covalently attachment doxorubicin via hydrazone bond (PEG-hyd-DOX) was prepared for tumor targeting delivery system. While PEG-DOX conjugates via amid bond (PEG-ami-DOX) was synthesized as control.<h4>Results</h4>The synthetic conjugates were confirmed by proton nuclear magnetic resonance (NMR) spectroscopy, the release profile of DOX from PEG-hyd-DOX was acid-liable for the hydrazone linkage between DOX and PEG, led to different intracellular uptake route; intracellular accumulation of PEG-hyd-DOX was higher than PEG-ami-DOX due to its pH-triggered profile, and thereby more cytotoxicity against MCF-7, MDA-MB-231 (breast cancer models) and HepG2 (hepatocellular carcinoma model) cell lines. Following the in vitro results, we xenografted MDA-MB-231 cell onto SCID mice, PEG-hyd-DOX showed stronger antitumor efficacy than free DOX and was tumor-targeting.<h4>Conclusions</h4>Results from these in vivo experiments were consistent with our in vitro results; suggested this pH-triggered PEG-hyd-DOX conjugate could target DOX to tumor tissues and release free drugs by acidic tumor environment, which would be potent in antitumor drug delivery. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028490/?tool=EBI |
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