Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats
Context Chloranthus serratus [(Thunb.) Roem. et Schult, (Chloranthaceae)] is a folk medicine used for the treatment of rheumatoid arthritis. Objective The aim of this study was to investigate anti-arthritic effects of the ethanol extracts of the roots (ER), stems (ES) and leaves (EL) of C. serratus...
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doaj-cccdbf71c1144773b1f971258ea6d7d02021-05-06T15:44:47ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162020-01-0158152853710.1080/13880209.2020.17671591767159Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in ratsShuping Sun0Shengli Li1Yunyan Du2Chenguang Wu3Mengyuan Zhang4Jiarong Li5Xiaoping Zhang6College of Pharmacy, Wannan Medical CollegeThe Fifth People’s Hospital of WuhuCollege of Pharmacy, Wannan Medical CollegeCollege of Pharmacy, Wannan Medical CollegeCollege of Pharmacy, Wannan Medical CollegeCollege of Pharmacy, Wannan Medical CollegeCollege of Life Science, Anhui Normal UniversityContext Chloranthus serratus [(Thunb.) Roem. et Schult, (Chloranthaceae)] is a folk medicine used for the treatment of rheumatoid arthritis. Objective The aim of this study was to investigate anti-arthritic effects of the ethanol extracts of the roots (ER), stems (ES) and leaves (EL) of C. serratus on adjuvant arthritis rats and related mechanisms. Materials and methods The rats were immunized by intradermal injection of complete Freund’s adjuvant (CFA, 0.18 mL) into the right hind feet, and received intragastric administrations of the ER, ES and EL (2.07, 1.61 and 0.58 g/kg/d, respectively) for 14 days. The anti-arthritic activity was assessed by swelling rates, serum indicators, antioxidant capacity, histopathological and immunohistochemical analyses. Results The LD50 of the ER, ES and EL was higher than 10.35, 8.05 and 2.90 g/kg/p.o., respectively. Extract treatments decreased swelling rates, tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), interleukin 1 beta (IL-1β), migration inhibitory factor 1 (MIF-1), immunoglobulin G (IgG) and immunoglobulin M (IgM) levels and positive expression of VEGF in the arthritic rats (p < 0.01 or p < 0.05). The ER significantly decreased NO (3.91 ± 0.61 µmol/L), IL-6 (75.67 ± 16.83 pg/mL) and malondialdehyde (MDA) (2.28 ± 0.32 nmol/mL) contents and clearly increased IFN-γ (2082 ± 220.93 pg/mL) and superoxide dismutase (SOD) (601.98 ± 38.40 U/mL) levels. The ES and EL did not reverse the changes in some indicators. All the extracts alleviated inflammatory cell infiltration and synovial cell proliferation. Among them, the ER was the most pronounced. Discussion and conclusions ER exerts the most promising effects, as shown by inhibiting the releases of inflammatory cytokines and enhancing antioxidant capacity, which provides a scientific basis for further research on C. serratus and its clinical applications.http://dx.doi.org/10.1080/13880209.2020.1767159rheumatoid arthritiscomplete freund’s adjuvantpro-inflammatory mediatorsoxidative stressarticular cartilagearthritic score |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuping Sun Shengli Li Yunyan Du Chenguang Wu Mengyuan Zhang Jiarong Li Xiaoping Zhang |
spellingShingle |
Shuping Sun Shengli Li Yunyan Du Chenguang Wu Mengyuan Zhang Jiarong Li Xiaoping Zhang Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats Pharmaceutical Biology rheumatoid arthritis complete freund’s adjuvant pro-inflammatory mediators oxidative stress articular cartilage arthritic score |
author_facet |
Shuping Sun Shengli Li Yunyan Du Chenguang Wu Mengyuan Zhang Jiarong Li Xiaoping Zhang |
author_sort |
Shuping Sun |
title |
Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats |
title_short |
Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats |
title_full |
Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats |
title_fullStr |
Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats |
title_full_unstemmed |
Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats |
title_sort |
anti-inflammatory effects of the root, stem and leaf extracts of chloranthus serratus on adjuvant-induced arthritis in rats |
publisher |
Taylor & Francis Group |
series |
Pharmaceutical Biology |
issn |
1388-0209 1744-5116 |
publishDate |
2020-01-01 |
description |
Context Chloranthus serratus [(Thunb.) Roem. et Schult, (Chloranthaceae)] is a folk medicine used for the treatment of rheumatoid arthritis. Objective The aim of this study was to investigate anti-arthritic effects of the ethanol extracts of the roots (ER), stems (ES) and leaves (EL) of C. serratus on adjuvant arthritis rats and related mechanisms. Materials and methods The rats were immunized by intradermal injection of complete Freund’s adjuvant (CFA, 0.18 mL) into the right hind feet, and received intragastric administrations of the ER, ES and EL (2.07, 1.61 and 0.58 g/kg/d, respectively) for 14 days. The anti-arthritic activity was assessed by swelling rates, serum indicators, antioxidant capacity, histopathological and immunohistochemical analyses. Results The LD50 of the ER, ES and EL was higher than 10.35, 8.05 and 2.90 g/kg/p.o., respectively. Extract treatments decreased swelling rates, tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), interleukin 1 beta (IL-1β), migration inhibitory factor 1 (MIF-1), immunoglobulin G (IgG) and immunoglobulin M (IgM) levels and positive expression of VEGF in the arthritic rats (p < 0.01 or p < 0.05). The ER significantly decreased NO (3.91 ± 0.61 µmol/L), IL-6 (75.67 ± 16.83 pg/mL) and malondialdehyde (MDA) (2.28 ± 0.32 nmol/mL) contents and clearly increased IFN-γ (2082 ± 220.93 pg/mL) and superoxide dismutase (SOD) (601.98 ± 38.40 U/mL) levels. The ES and EL did not reverse the changes in some indicators. All the extracts alleviated inflammatory cell infiltration and synovial cell proliferation. Among them, the ER was the most pronounced. Discussion and conclusions ER exerts the most promising effects, as shown by inhibiting the releases of inflammatory cytokines and enhancing antioxidant capacity, which provides a scientific basis for further research on C. serratus and its clinical applications. |
topic |
rheumatoid arthritis complete freund’s adjuvant pro-inflammatory mediators oxidative stress articular cartilage arthritic score |
url |
http://dx.doi.org/10.1080/13880209.2020.1767159 |
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