Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves
There is continued debate regarding the appropriate cell type to replace valvular interstitial cells (VICs) in tissue engineered heart valves (TEHVs), particularly for pediatric patients. In this work, neonatal human dermal fibroblasts (nhDFFs) were compared to human pediatric VICs (hpVICs), based o...
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doaj-ccd0cfb163d34d2e907d2e286072cee92020-11-24T23:04:20ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252014-08-011214616210.3390/jcdd1020146jcdd1020146Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart ValvesMonica M. Fahrenholtz0Huiwen Liu1Debra L. Kearney2Lalita Wadhwa3Charles D. Fraser4K. Jane Grande-Allen5Department of Bioengineering, Rice University, 6100 Main Street MS-142, Houston, TX 77005, USADepartment of Bioengineering, Rice University, 6100 Main Street MS-142, Houston, TX 77005, USADepartment of Pathology, Texas Children's Hospital, 6621 Fannin Street, Houston, TX 77030, USADepartment of Congenital Heart Surgery, Texas Children's Hospital, 6621 Fannin Street, Houston, TX 77030, USADepartment of Congenital Heart Surgery, Texas Children's Hospital, 6621 Fannin Street, Houston, TX 77030, USADepartment of Bioengineering, Rice University, 6100 Main Street MS-142, Houston, TX 77005, USAThere is continued debate regarding the appropriate cell type to replace valvular interstitial cells (VICs) in tissue engineered heart valves (TEHVs), particularly for pediatric patients. In this work, neonatal human dermal fibroblasts (nhDFFs) were compared to human pediatric VICs (hpVICs), based on their phenotypic and gene expression characteristics when cultured on collagen type I, fibronectin, fibrin, and tissue culture polystyrene (TCP) substrates. Similar confluency was achieved over the culture period on collagen and fibronectin between both cell types, although nhDFFs tended to reach lower confluence on collagen than on any other substrate. Morphologically, hpVICs tended to spread and form multiple extensions, while nhDFFs remained homogenously spindle-shaped on all substrates. PCR results indicated that fibroblasts did not differ significantly from VICs in gene expression when cultured on fibrin, whereas on collagen type I and fibronectin they showed increased α-SMA, xylosyltransferase I, and collagen type I expression (p < 0.05). However, protein expression of these targets, analyzed by immunocytochemistry and Western blotting, was not significantly different between cell types. These results suggest that nhDFFs express similar matrix production and remodeling genes as hpVICs, and the choice of substrate for TEHV construction can affect the growth and expression profile of nhDFFs as compared to native hpVICs.http://www.mdpi.com/2308-3425/1/2/146fibroblastspediatricvalvular interstitial cellVICTEHVextracellular matrixsubstrate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Monica M. Fahrenholtz Huiwen Liu Debra L. Kearney Lalita Wadhwa Charles D. Fraser K. Jane Grande-Allen |
spellingShingle |
Monica M. Fahrenholtz Huiwen Liu Debra L. Kearney Lalita Wadhwa Charles D. Fraser K. Jane Grande-Allen Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves Journal of Cardiovascular Development and Disease fibroblasts pediatric valvular interstitial cell VIC TEHV extracellular matrix substrate |
author_facet |
Monica M. Fahrenholtz Huiwen Liu Debra L. Kearney Lalita Wadhwa Charles D. Fraser K. Jane Grande-Allen |
author_sort |
Monica M. Fahrenholtz |
title |
Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves |
title_short |
Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves |
title_full |
Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves |
title_fullStr |
Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves |
title_full_unstemmed |
Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves |
title_sort |
characterization of dermal fibroblasts as a cell source for pediatric tissue engineered heart valves |
publisher |
MDPI AG |
series |
Journal of Cardiovascular Development and Disease |
issn |
2308-3425 |
publishDate |
2014-08-01 |
description |
There is continued debate regarding the appropriate cell type to replace valvular interstitial cells (VICs) in tissue engineered heart valves (TEHVs), particularly for pediatric patients. In this work, neonatal human dermal fibroblasts (nhDFFs) were compared to human pediatric VICs (hpVICs), based on their phenotypic and gene expression characteristics when cultured on collagen type I, fibronectin, fibrin, and tissue culture polystyrene (TCP) substrates. Similar confluency was achieved over the culture period on collagen and fibronectin between both cell types, although nhDFFs tended to reach lower confluence on collagen than on any other substrate. Morphologically, hpVICs tended to spread and form multiple extensions, while nhDFFs remained homogenously spindle-shaped on all substrates. PCR results indicated that fibroblasts did not differ significantly from VICs in gene expression when cultured on fibrin, whereas on collagen type I and fibronectin they showed increased α-SMA, xylosyltransferase I, and collagen type I expression (p < 0.05). However, protein expression of these targets, analyzed by immunocytochemistry and Western blotting, was not significantly different between cell types. These results suggest that nhDFFs express similar matrix production and remodeling genes as hpVICs, and the choice of substrate for TEHV construction can affect the growth and expression profile of nhDFFs as compared to native hpVICs. |
topic |
fibroblasts pediatric valvular interstitial cell VIC TEHV extracellular matrix substrate |
url |
http://www.mdpi.com/2308-3425/1/2/146 |
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