Evaluation of the Functional Activity of the Specific Anticholeraic Enterosorbent

• Main Authors: M. V. Ovchinnikova, M. N. Islyaeva, V. G. Nikolaev, K. I. Bardakhivskaya, A. K. Nikiforov
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2015-06-01
• Series: Проблемы особо опасных инфекций
• Subjects: энтеросорбенты; специфические энтеросорбенты; хитозан; функциональная активность; фармакологические маркеры; холерный токсин; специфическая сорбция; enterosorbents; specific enterosorbents; chitosan; functional activity; pharmacological markers; cholera toxin; specific sorption
• Online Access: https://journal.microbe.ru/jour/article/view/233

Carried out is comparative analysis of adsorption activity of the constructed specific enterosorbent as regards pharmacological markers - methylene blue, bovine serum albumin, gelatin, and neutralizing activity in vivo with reference to the specific marker - cholera toxin. The data obtained testify to the fact that the level of the sorption activity in relation to the utilized marker substances both of initial micro-particles of chitosan, and its specific modification, is comparable to the corresponding parameter of the reference drugs. It is established that the process of immunoglobulin component immobilization initiates the changes in electro-chemical properties of the chitosan active surface, leading, on the one side, to the decrease in the sorption activity of the specific preparation in relation to methylene blue and gelatin, and on the other, to the increase in the protein marker sorption activity - bovine serum albumin, as compared to the initial chitosan matrix. In the course of the toxin-neutralizing capacity investigation, identified has been high anti-toxic activity of the experimental preparation in reference to cholera vibrio enterotoxin. The results attained give grounds to view the constructed anti-enterotoxic enterosorbent as potential prototype of efficient aids for cholera prophylaxis and treatment.