Long Noncoding RNA KCNMB2-AS1 Promotes SMAD5 by Targeting miR-3194-3p to Induce Bladder Cancer Progression
PurposeBladder cancer is a common malignant tumor of the urinary system, with the fourth-highest incidence of male malignant tumors in Europe and the United States. So far, the mechanism of bladder cancer progression and metastasis has not been clarified. The aim of our study was to validate the way...
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doaj-ccd95495e100421d8ca7deb50aac79722021-05-07T08:28:06ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-05-011110.3389/fonc.2021.649778649778Long Noncoding RNA KCNMB2-AS1 Promotes SMAD5 by Targeting miR-3194-3p to Induce Bladder Cancer ProgressionYong-Sheng Chen0Yong-Peng Xu1Wen-Hua Liu2De-Chao Li3Huan Wang4Chang-Fu Li5Department of Urology, Harbin Medical University Cancer Hospital, Harbin, ChinaDepartment of Urology, Harbin Medical University Cancer Hospital, Harbin, ChinaIntensive Care Unit (ICU) Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Urology, Harbin Medical University Cancer Hospital, Harbin, ChinaDepartment of Urology, Harbin Medical University Cancer Hospital, Harbin, ChinaDepartment of Urology, Harbin Medical University Cancer Hospital, Harbin, ChinaPurposeBladder cancer is a common malignant tumor of the urinary system, with the fourth-highest incidence of male malignant tumors in Europe and the United States. So far, the mechanism of bladder cancer progression and metastasis has not been clarified. The aim of our study was to validate the way of long noncoding RNA (lncRNA) KCNMB2-AS1 on the metabolism and growth of bladder cancer cells by miR-3194-3p/SMAD5.Patients and MethodsThe Gene Expression was analyzed by qRT-PCR in bladder cancer tissues and cell lines, with the highly expressed KCNMB2-AS1 screened out. Cell proliferation was detected by Edu staining and clone formation assay, cell migration, and invasion by wound healing and transwell assays. Cell stemness was determined by assessing sphere-forming ability and stemness marker. Correlation between miRNA and lncRNA/gene was verified by dual‐luciferase assay and RIP, and the effect of KCNMB2-AS1 on bladder cancer growth by nude mice tumor formation experiment.ResultsHere, we revealed the increased level of KCNMB2-AS1 in bladder cancer for the first time. Knockdown of KCNMB2-AS1 in vitro prevented the ability of proliferation, metastasis, and stemness of cancer cells. In vivo, the silencing of KCNMB2-AS1 also prevented tumor growth in vivo. Next, we revealed that KCNMB2-AS1 could interact with miR-3194-3p and uncovered that SAMD5 was a downstream target of miR-3194-3p.ConclusionIn conclusion, KCNMB2-AS1 mediated the bladder cancer cells progress by regulating the miR-3194-3p/SAMD5 signal pathway, which would provide a new target for bladder cancer research.https://www.frontiersin.org/articles/10.3389/fonc.2021.649778/fullbladder cancerKCNMB2-AS1miR-3194-3pstemnessSAMD5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong-Sheng Chen Yong-Peng Xu Wen-Hua Liu De-Chao Li Huan Wang Chang-Fu Li |
spellingShingle |
Yong-Sheng Chen Yong-Peng Xu Wen-Hua Liu De-Chao Li Huan Wang Chang-Fu Li Long Noncoding RNA KCNMB2-AS1 Promotes SMAD5 by Targeting miR-3194-3p to Induce Bladder Cancer Progression Frontiers in Oncology bladder cancer KCNMB2-AS1 miR-3194-3p stemness SAMD5 |
author_facet |
Yong-Sheng Chen Yong-Peng Xu Wen-Hua Liu De-Chao Li Huan Wang Chang-Fu Li |
author_sort |
Yong-Sheng Chen |
title |
Long Noncoding RNA KCNMB2-AS1 Promotes SMAD5 by Targeting miR-3194-3p to Induce Bladder Cancer Progression |
title_short |
Long Noncoding RNA KCNMB2-AS1 Promotes SMAD5 by Targeting miR-3194-3p to Induce Bladder Cancer Progression |
title_full |
Long Noncoding RNA KCNMB2-AS1 Promotes SMAD5 by Targeting miR-3194-3p to Induce Bladder Cancer Progression |
title_fullStr |
Long Noncoding RNA KCNMB2-AS1 Promotes SMAD5 by Targeting miR-3194-3p to Induce Bladder Cancer Progression |
title_full_unstemmed |
Long Noncoding RNA KCNMB2-AS1 Promotes SMAD5 by Targeting miR-3194-3p to Induce Bladder Cancer Progression |
title_sort |
long noncoding rna kcnmb2-as1 promotes smad5 by targeting mir-3194-3p to induce bladder cancer progression |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2021-05-01 |
description |
PurposeBladder cancer is a common malignant tumor of the urinary system, with the fourth-highest incidence of male malignant tumors in Europe and the United States. So far, the mechanism of bladder cancer progression and metastasis has not been clarified. The aim of our study was to validate the way of long noncoding RNA (lncRNA) KCNMB2-AS1 on the metabolism and growth of bladder cancer cells by miR-3194-3p/SMAD5.Patients and MethodsThe Gene Expression was analyzed by qRT-PCR in bladder cancer tissues and cell lines, with the highly expressed KCNMB2-AS1 screened out. Cell proliferation was detected by Edu staining and clone formation assay, cell migration, and invasion by wound healing and transwell assays. Cell stemness was determined by assessing sphere-forming ability and stemness marker. Correlation between miRNA and lncRNA/gene was verified by dual‐luciferase assay and RIP, and the effect of KCNMB2-AS1 on bladder cancer growth by nude mice tumor formation experiment.ResultsHere, we revealed the increased level of KCNMB2-AS1 in bladder cancer for the first time. Knockdown of KCNMB2-AS1 in vitro prevented the ability of proliferation, metastasis, and stemness of cancer cells. In vivo, the silencing of KCNMB2-AS1 also prevented tumor growth in vivo. Next, we revealed that KCNMB2-AS1 could interact with miR-3194-3p and uncovered that SAMD5 was a downstream target of miR-3194-3p.ConclusionIn conclusion, KCNMB2-AS1 mediated the bladder cancer cells progress by regulating the miR-3194-3p/SAMD5 signal pathway, which would provide a new target for bladder cancer research. |
topic |
bladder cancer KCNMB2-AS1 miR-3194-3p stemness SAMD5 |
url |
https://www.frontiersin.org/articles/10.3389/fonc.2021.649778/full |
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