Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India
Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue...
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Elsevier
2019-07-01
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Series: | International Journal of Infectious Diseases |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971219300293 |
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Sivaram Gunisetty Kaustuv Nayak Ramesh Chandra Rai Yadya Chawla Elluri Seetharami Reddy Charu Aggarwal Deepti Maheshwari Harekrushna Panda Nasim Akhtar Ansari Prabhat Singh Manpreet Kaur Kritika Dixit Pragati Sharma Priya Bhatnagar Lalita Priyamvada Siddhartha Kumar Bhaumik Syed Fazil Ahamed Rosario Vivek Pratima Ray Anita Shet Poonam Coshic Rakesh Lodha Sushil Kumar Kabra Dil Afroze Adfar Yousuf Rafi Ahmed Kaja Murali-Krishna Anmol Chandele |
spellingShingle |
Sivaram Gunisetty Kaustuv Nayak Ramesh Chandra Rai Yadya Chawla Elluri Seetharami Reddy Charu Aggarwal Deepti Maheshwari Harekrushna Panda Nasim Akhtar Ansari Prabhat Singh Manpreet Kaur Kritika Dixit Pragati Sharma Priya Bhatnagar Lalita Priyamvada Siddhartha Kumar Bhaumik Syed Fazil Ahamed Rosario Vivek Pratima Ray Anita Shet Poonam Coshic Rakesh Lodha Sushil Kumar Kabra Dil Afroze Adfar Yousuf Rafi Ahmed Kaja Murali-Krishna Anmol Chandele Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India International Journal of Infectious Diseases |
author_facet |
Sivaram Gunisetty Kaustuv Nayak Ramesh Chandra Rai Yadya Chawla Elluri Seetharami Reddy Charu Aggarwal Deepti Maheshwari Harekrushna Panda Nasim Akhtar Ansari Prabhat Singh Manpreet Kaur Kritika Dixit Pragati Sharma Priya Bhatnagar Lalita Priyamvada Siddhartha Kumar Bhaumik Syed Fazil Ahamed Rosario Vivek Pratima Ray Anita Shet Poonam Coshic Rakesh Lodha Sushil Kumar Kabra Dil Afroze Adfar Yousuf Rafi Ahmed Kaja Murali-Krishna Anmol Chandele |
author_sort |
Sivaram Gunisetty |
title |
Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India |
title_short |
Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India |
title_full |
Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India |
title_fullStr |
Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India |
title_full_unstemmed |
Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India |
title_sort |
analysis of dengue specific memory b cells, neutralizing antibodies and binding antibodies in healthy adults from india |
publisher |
Elsevier |
series |
International Journal of Infectious Diseases |
issn |
1201-9712 |
publishDate |
2019-07-01 |
description |
Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region. Methods: We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n = 70) from New Delhi. Results: NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50–1:30,264), binding antibodies (range, 1:3,000–1:134,000,) as well as the MBC (range = 0.006%–5.05%). Conclusion: These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs – emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines. Keywords: Dengue, India, Neutralizing antibody, Memory B-cell |
url |
http://www.sciencedirect.com/science/article/pii/S1201971219300293 |
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doaj-ccda8c5005984bc7abfe03d7ab6696922020-11-24T21:54:37ZengElsevierInternational Journal of Infectious Diseases1201-97122019-07-0184S57S63Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from IndiaSivaram Gunisetty0Kaustuv Nayak1Ramesh Chandra Rai2Yadya Chawla3Elluri Seetharami Reddy4Charu Aggarwal5Deepti Maheshwari6Harekrushna Panda7Nasim Akhtar Ansari8Prabhat Singh9Manpreet Kaur10Kritika Dixit11Pragati Sharma12Priya Bhatnagar13Lalita Priyamvada14Siddhartha Kumar Bhaumik15Syed Fazil Ahamed16Rosario Vivek17Pratima Ray18Anita Shet19Poonam Coshic20Rakesh Lodha21Sushil Kumar Kabra22Dil Afroze23Adfar Yousuf24Rafi Ahmed25Kaja Murali-Krishna26Anmol Chandele27ICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India; Department of Pediatrics, Division of Infectious Disease, Emory University School of Medicine, Atlanta, GA, USA; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, 30322, USAICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, IndiaDepartment of Pediatrics, Division of Infectious Disease, Emory University School of Medicine, Atlanta, GA, USADepartment of Pediatrics, Division of Infectious Disease, Emory University School of Medicine, Atlanta, GA, USADivision of Infectious Diseases, St. John’s Research Institute, St. John’s National Academy of Health Sciences, Koramangala, Bangalore, 560034, India; The University of Trans-Disciplinary Health Sciences & Technology, Bangalore, 560064, Karnataka, IndiaDivision of Infectious Diseases, St. John’s Research Institute, St. John’s National Academy of Health Sciences, Koramangala, Bangalore, 560034, IndiaDepartment of Biotechnology, School of Clinical and Life Sciences, Jamia Hamdard, Mehrauli-Bardarpur Road, New Delhi, 110062, IndiaDivision of Infectious Diseases, St. John’s Research Institute, St. John’s National Academy of Health Sciences, Koramangala, Bangalore, 560034, India; International Vaccine Access Center, Johns Hopkins Bloomberg School of Public Health, 415 N Washington St, Baltimore, 21231, USADepartment of Transfusion Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, IndiaDivision of Pediatric Pulmonology and Intensive Care, Department of Pediatrics, AIIMS, New Delhi, IndiaDivision of Pediatric Pulmonology and Intensive Care, Department of Pediatrics, AIIMS, New Delhi, IndiaImmunology & Molecular Medicine, Sher-i-Kashmir Institute of Medical Sciences-Srinagar, Jammu and Kashmir, 190011, IndiaImmunology & Molecular Medicine, Sher-i-Kashmir Institute of Medical Sciences-Srinagar, Jammu and Kashmir, 190011, IndiaEmory Vaccine Center, Emory University School of Medicine, Atlanta, GA, 30322, USA; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, 30322, USAICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India; Department of Pediatrics, Division of Infectious Disease, Emory University School of Medicine, Atlanta, GA, USA; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, 30322, USA; Corresponding authors at: ICGEB-Emory Vaccine Center, International center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India; Department of Pediatrics, Division of Infectious Disease, Emory University School of Medicine, Atlanta, GA 30322, USA.ICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India; Corresponding authors.Background: The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region. Methods: We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n = 70) from New Delhi. Results: NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50–1:30,264), binding antibodies (range, 1:3,000–1:134,000,) as well as the MBC (range = 0.006%–5.05%). Conclusion: These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs – emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines. Keywords: Dengue, India, Neutralizing antibody, Memory B-cellhttp://www.sciencedirect.com/science/article/pii/S1201971219300293 |