Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa
Background: Antimicrobial resistance is one of the major characteristics of infectious agents. Silver nanoparticles (AgNPs) have been introduced as novel antibacterial agents in accordance with the traditional treatments. Our purpose of this study was to evaluate the antimicrobial activity of AgNPs...
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doaj-ccf05debd7404f5b838734abe83a1ac92020-11-24T23:04:20ZengZabol University of Medical sciencesInternational Journal of Basic Science in Medicine2476-664X2016-06-0111252810.15171/ijbsm.2016.06IJBSM-1-20160701040235Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosaAliakbar Nasiri0Roghayeh Afsar Gharebagh1Seyed Ali Nojoumi2Majidreza Akbarizadeh3Sharareh Harirchi4Masoud Arefnezhad5Shahla Sahraei6Mehran Hesaraki7Mahdi Afshari8Fereshteh Javadian9Maryam Sheykhzade Asadi10Zahra Shahi11Aliyeh Sargazi12Assistant Professor of Anesthesiology, Department of Anesthesiology, Zabol University of Medical Sciences, Zabol, IranAssistant Professor of Cardiology, Department of Cardiology, Urmia University of Medical Sciences, Urmia, IranAssistant Professor of Microbiology, Microbiology Research Center, Pasteur Institute of Iran, Tehran, IranAssistant Professor of Pediatrics, Department of Pediatrics, Zabol University of Medical Sciences, Zabol, IranPhD Student of Microbiology, Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, IranZabol University of Medical Sciences, Zabol, IranZabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, IranAssistant Professor of Pediatrics, Department of Pediatrics, Zabol University of Medical Sciences, Zabol, IranZabol University of Medical Sciences, Zabol, IranZabol University of Medical Sciences, Zabol, IranKerman Science and Research Branch, Islamic Azad University, Kerman, IranKerman Science and Research Branch, Islamic Azad University, Kerman, Iran Student Research Committee, Zabol University of Medical Sciences, Zabol, IranBackground: Antimicrobial resistance is one of the major characteristics of infectious agents. Silver nanoparticles (AgNPs) have been introduced as novel antibacterial agents in accordance with the traditional treatments. Our purpose of this study was to evaluate the antimicrobial activity of AgNPs on the Pseudomonas aeruginosa (P. aeruginosa) that are resistant to antibiotics. Methods: During a cross-sectional study, we tried to evaluate 20 strains of P. aeruginosa isolated from the urine cultures of patients admitted to the hospital due to urinary tract infections. The AgNPs were commercially purchased. The minimum inhibitory concentration (MIC) of AgNPs in different concentrations was determined by the dilution in wells on bacteria. The antibiotic susceptibility pattern of P. aeruginosa was evaluated by the Kirby-Bauer disk diffusion standard. Results: Current study indicated that P. aeruginosa were resistant to four types of agents including ampicillin (85%), nitrofurantoin (65%), nalidixic acid (65%), and ciprofloxacin (15%) and result of nanosilver indicated that the most MIC was 100 ppm concentration, and six strains of P. aeruginosa were inhibited by it. Conclusion: Our study presented a new type of silver nanoparticle and indicated that they can be embedded in bone cement to prevent infections once synthetic conditions are tailored for such applications.http://ijbsm.zbmu.ac.ir/PDF/IJBSM-1-25.pdfNanosilverAntimicrobial activityPseudomonas aeruginosaMinimum inhibitory concentrations (MIC)Antibiotic-resistant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aliakbar Nasiri Roghayeh Afsar Gharebagh Seyed Ali Nojoumi Majidreza Akbarizadeh Sharareh Harirchi Masoud Arefnezhad Shahla Sahraei Mehran Hesaraki Mahdi Afshari Fereshteh Javadian Maryam Sheykhzade Asadi Zahra Shahi Aliyeh Sargazi |
spellingShingle |
Aliakbar Nasiri Roghayeh Afsar Gharebagh Seyed Ali Nojoumi Majidreza Akbarizadeh Sharareh Harirchi Masoud Arefnezhad Shahla Sahraei Mehran Hesaraki Mahdi Afshari Fereshteh Javadian Maryam Sheykhzade Asadi Zahra Shahi Aliyeh Sargazi Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa International Journal of Basic Science in Medicine Nanosilver Antimicrobial activity Pseudomonas aeruginosa Minimum inhibitory concentrations (MIC) Antibiotic-resistant |
author_facet |
Aliakbar Nasiri Roghayeh Afsar Gharebagh Seyed Ali Nojoumi Majidreza Akbarizadeh Sharareh Harirchi Masoud Arefnezhad Shahla Sahraei Mehran Hesaraki Mahdi Afshari Fereshteh Javadian Maryam Sheykhzade Asadi Zahra Shahi Aliyeh Sargazi |
author_sort |
Aliakbar Nasiri |
title |
Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa |
title_short |
Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa |
title_full |
Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa |
title_fullStr |
Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa |
title_full_unstemmed |
Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa |
title_sort |
evaluation of the antimicrobial activity of silver nanoparticles on antibiotic-resistant pseudomonas aeruginosa |
publisher |
Zabol University of Medical sciences |
series |
International Journal of Basic Science in Medicine |
issn |
2476-664X |
publishDate |
2016-06-01 |
description |
Background: Antimicrobial resistance is one of the major characteristics of infectious agents. Silver nanoparticles (AgNPs) have been introduced as novel antibacterial agents in accordance with the traditional treatments. Our purpose of this study was to evaluate the antimicrobial activity of AgNPs on the Pseudomonas aeruginosa (P. aeruginosa) that are resistant to antibiotics.
Methods: During a cross-sectional study, we tried to evaluate 20 strains of P. aeruginosa isolated from the urine cultures of patients admitted to the hospital due to urinary tract infections. The AgNPs were commercially purchased. The minimum inhibitory concentration (MIC) of AgNPs in different concentrations was determined by the dilution in wells on bacteria. The antibiotic susceptibility pattern of P. aeruginosa was evaluated by the Kirby-Bauer disk diffusion standard.
Results: Current study indicated that P. aeruginosa were resistant to four types of agents including ampicillin (85%), nitrofurantoin (65%), nalidixic acid (65%), and ciprofloxacin (15%) and result of nanosilver indicated that the most MIC was 100 ppm concentration, and six strains of P. aeruginosa were inhibited by it.
Conclusion: Our study presented a new type of silver nanoparticle and indicated that they can be embedded in bone cement to prevent infections once synthetic conditions are tailored for such applications. |
topic |
Nanosilver Antimicrobial activity Pseudomonas aeruginosa Minimum inhibitory concentrations (MIC) Antibiotic-resistant |
url |
http://ijbsm.zbmu.ac.ir/PDF/IJBSM-1-25.pdf |
work_keys_str_mv |
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