In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch
Recently, the interest in discovering natural antioxidants has increased tremendously for their application to control onset of multiple diseases. The present study was designed to determine polyphenolic compounds and antioxidant potential of Hedera nepalensis. Crude extract of H. nepalensis and its...
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doaj-cd6800555a8f4b50a782a676c243296b2020-11-24T23:45:48ZengElsevierArabian Journal of Chemistry1878-53522017-05-0110S2S3699S370610.1016/j.arabjc.2014.05.002In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. KochLaila Jafri0Samreen Saleem1Ihsan-ul-Haq2Nazif Ullah3Bushra Mirza4Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biotechnology, Abdul Wali Khan University, Mardan, PakistanDepartment of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanRecently, the interest in discovering natural antioxidants has increased tremendously for their application to control onset of multiple diseases. The present study was designed to determine polyphenolic compounds and antioxidant potential of Hedera nepalensis. Crude extract of H. nepalensis and its fractions (n-hexane, ethyl acetate and aqueous) was prepared by solvent–solvent extraction. Total flavonoid and phenolic contents were determined by colorimetric methods using quercetin and gallic acid as standard. Further quantitative analysis of phenolic and flavonoid compounds was carried out by using high performance liquid chromatography coupled with diode array detector (HPLC-DAD). Ethyl acetate fraction showed the highest amount of total flavonoid (2.4 ± 0.164 mg QE/100 mg) and phenolic contents (12.90 ± 0.15 mg GAE/100 mg). Using HPLC-DAD, catechin was identified in aqueous fraction and caffeic acid was identified in ethyl acetate fraction of H. nepalensis. The antioxidant capacity of H. nepalensis was evaluated by measuring the scavenging potential of hydrogen peroxide (H2O2), total antioxidant capacity and reducing power. The extract/fractions showed significant (P < 0.05) hydrogen peroxide scavenging potential with their IC50 values ranging from 31.19 to 200 μg/ml. Among crude extract and all the fractions of H. nepalensis, ethyl acetate fraction showed the highest total antioxidant activity by phosphomolybdenum method followed by n-hexane, crude extract and aqueous fraction. Moreover, ethyl acetate fraction has shown the highest reducing power followed by aqueous fraction, n-hexane fraction and crude extract. The present study provides evidence that ethyl acetate fraction of H. nepalensis has significant antioxidant potential which correlates well with its high phenolic and flavonoid contents. To the best of our knowledge catechin and caffeic acid have been reported for the first time in H. nepalensis.http://www.sciencedirect.com/science/article/pii/S1878535214000781Antioxidant assaysHedera nepalensisHPLC-DAD analysisFlavonoidsPhenolic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laila Jafri Samreen Saleem Ihsan-ul-Haq Nazif Ullah Bushra Mirza |
spellingShingle |
Laila Jafri Samreen Saleem Ihsan-ul-Haq Nazif Ullah Bushra Mirza In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch Arabian Journal of Chemistry Antioxidant assays Hedera nepalensis HPLC-DAD analysis Flavonoids Phenolic |
author_facet |
Laila Jafri Samreen Saleem Ihsan-ul-Haq Nazif Ullah Bushra Mirza |
author_sort |
Laila Jafri |
title |
In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch |
title_short |
In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch |
title_full |
In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch |
title_fullStr |
In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch |
title_full_unstemmed |
In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch |
title_sort |
in vitro assessment of antioxidant potential and determination of polyphenolic compounds of hedera nepalensis k. koch |
publisher |
Elsevier |
series |
Arabian Journal of Chemistry |
issn |
1878-5352 |
publishDate |
2017-05-01 |
description |
Recently, the interest in discovering natural antioxidants has increased tremendously for their application to control onset of multiple diseases. The present study was designed to determine polyphenolic compounds and antioxidant potential of Hedera nepalensis. Crude extract of H. nepalensis and its fractions (n-hexane, ethyl acetate and aqueous) was prepared by solvent–solvent extraction. Total flavonoid and phenolic contents were determined by colorimetric methods using quercetin and gallic acid as standard. Further quantitative analysis of phenolic and flavonoid compounds was carried out by using high performance liquid chromatography coupled with diode array detector (HPLC-DAD). Ethyl acetate fraction showed the highest amount of total flavonoid (2.4 ± 0.164 mg QE/100 mg) and phenolic contents (12.90 ± 0.15 mg GAE/100 mg). Using HPLC-DAD, catechin was identified in aqueous fraction and caffeic acid was identified in ethyl acetate fraction of H. nepalensis. The antioxidant capacity of H. nepalensis was evaluated by measuring the scavenging potential of hydrogen peroxide (H2O2), total antioxidant capacity and reducing power. The extract/fractions showed significant (P < 0.05) hydrogen peroxide scavenging potential with their IC50 values ranging from 31.19 to 200 μg/ml. Among crude extract and all the fractions of H. nepalensis, ethyl acetate fraction showed the highest total antioxidant activity by phosphomolybdenum method followed by n-hexane, crude extract and aqueous fraction. Moreover, ethyl acetate fraction has shown the highest reducing power followed by aqueous fraction, n-hexane fraction and crude extract. The present study provides evidence that ethyl acetate fraction of H. nepalensis has significant antioxidant potential which correlates well with its high phenolic and flavonoid contents. To the best of our knowledge catechin and caffeic acid have been reported for the first time in H. nepalensis. |
topic |
Antioxidant assays Hedera nepalensis HPLC-DAD analysis Flavonoids Phenolic |
url |
http://www.sciencedirect.com/science/article/pii/S1878535214000781 |
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