Liquid Hot Water Pretreatment of Miscanthus X giganteus for the Sustainable Production of Bioethanol
The production of ethanol from lignocellulosic biomass provides an alternative to fossil fuels. In this study, liquid hot water (LHW)-pretreated Miscanthus x giganteus (MxG) was used to produce bioethanol through simultaneous saccharification and fermentation (SSF). MxG was pretreated at temperature...
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North Carolina State University
2015-07-01
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doaj-cd8f9fbe854b4260aba1d607043fffe62020-11-24T22:47:27ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-07-011035890590510.15376/biores.10.3.5890-5905Liquid Hot Water Pretreatment of Miscanthus X giganteus for the Sustainable Production of BioethanolNana Abayie Boakye-Boaten0Shuangning Xiu1Abolghasem Shahbazi2Jonathan Fabish3Department of Natural Resources and Environmental Design, Biological Engineering Program; NC A&T State University; United StatesDepartment of Natural Resources and Environmental Design, Biological Engineering Program; NC A&T State University; United StatesDepartment of Natural Resources and Environmental Design, Biological Engineering Program; NC A&T State University; United StatesDepartment of Natural Resources and Environmental Design, Biological Engineering Program; NC A&T State University; United StatesThe production of ethanol from lignocellulosic biomass provides an alternative to fossil fuels. In this study, liquid hot water (LHW)-pretreated Miscanthus x giganteus (MxG) was used to produce bioethanol through simultaneous saccharification and fermentation (SSF). MxG was pretreated at temperatures between 170 and 200 °C, for 5 to 15 min. The pretreatment was able to remove between 68.3% and 77.0% of the lignin present in the biomass. The highest percentage yields of sugars from pretreated MxG after enzymatic saccharification (32 °C, pH 4.5, 48 h), by a cocktail of two enzymes were 44.0% glucose and 42.0% xylose of theoretical. Ethanol concentrations between 0.780 and 3.715 g/L, and a high ethanol yield of 71.8% of theoretical were obtained using Saccharomyces cerevisiae (ATCC 24858) for fermentation. A comparison of scanning electron micrographs of the pretreated biomass showed morphological changes that enhanced the release of glucose and bioethanol yield.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_5890_Boakye_Boaten_Liquid_Hot_Water_Pretreatment_MiscanthusLiquid hot water pretreatmentBioethanolSimultaneous saccharification and fermentationMiscanthus x giganteus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nana Abayie Boakye-Boaten Shuangning Xiu Abolghasem Shahbazi Jonathan Fabish |
spellingShingle |
Nana Abayie Boakye-Boaten Shuangning Xiu Abolghasem Shahbazi Jonathan Fabish Liquid Hot Water Pretreatment of Miscanthus X giganteus for the Sustainable Production of Bioethanol BioResources Liquid hot water pretreatment Bioethanol Simultaneous saccharification and fermentation Miscanthus x giganteus |
author_facet |
Nana Abayie Boakye-Boaten Shuangning Xiu Abolghasem Shahbazi Jonathan Fabish |
author_sort |
Nana Abayie Boakye-Boaten |
title |
Liquid Hot Water Pretreatment of Miscanthus X giganteus for the Sustainable Production of Bioethanol |
title_short |
Liquid Hot Water Pretreatment of Miscanthus X giganteus for the Sustainable Production of Bioethanol |
title_full |
Liquid Hot Water Pretreatment of Miscanthus X giganteus for the Sustainable Production of Bioethanol |
title_fullStr |
Liquid Hot Water Pretreatment of Miscanthus X giganteus for the Sustainable Production of Bioethanol |
title_full_unstemmed |
Liquid Hot Water Pretreatment of Miscanthus X giganteus for the Sustainable Production of Bioethanol |
title_sort |
liquid hot water pretreatment of miscanthus x giganteus for the sustainable production of bioethanol |
publisher |
North Carolina State University |
series |
BioResources |
issn |
1930-2126 1930-2126 |
publishDate |
2015-07-01 |
description |
The production of ethanol from lignocellulosic biomass provides an alternative to fossil fuels. In this study, liquid hot water (LHW)-pretreated Miscanthus x giganteus (MxG) was used to produce bioethanol through simultaneous saccharification and fermentation (SSF). MxG was pretreated at temperatures between 170 and 200 °C, for 5 to 15 min. The pretreatment was able to remove between 68.3% and 77.0% of the lignin present in the biomass. The highest percentage yields of sugars from pretreated MxG after enzymatic saccharification (32 °C, pH 4.5, 48 h), by a cocktail of two enzymes were 44.0% glucose and 42.0% xylose of theoretical. Ethanol concentrations between 0.780 and 3.715 g/L, and a high ethanol yield of 71.8% of theoretical were obtained using Saccharomyces cerevisiae (ATCC 24858) for fermentation. A comparison of scanning electron micrographs of the pretreated biomass showed morphological changes that enhanced the release of glucose and bioethanol yield. |
topic |
Liquid hot water pretreatment Bioethanol Simultaneous saccharification and fermentation Miscanthus x giganteus |
url |
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_5890_Boakye_Boaten_Liquid_Hot_Water_Pretreatment_Miscanthus |
work_keys_str_mv |
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