AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD
We have compared five different assays for antibodies to aquaporin-4 in 181 cases of suspected Neuromyelitis optica spectrum disorders (NMOSD) and 253 controls to assess their relative utility. As part of a clinically-based survey of NMOSD in Australia and New Zealand, cases of suspected NMOSD were...
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doaj-cd95a3512217467f9cd50b492ad5254f2020-11-24T20:45:00ZengFrontiers Media S.A.Frontiers in Neurology1664-22952019-10-011010.3389/fneur.2019.01028483793AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSDKerri Prain0Mark Woodhall1Angela Vincent2Sudarshini Ramanathan3Sudarshini Ramanathan4Michael H. Barnett5Christine S. Bundell6Christine S. Bundell7John D. E. Parratt8John D. E. Parratt9Roger A. Silvestrini10Wajih Bukhari11Wajih Bukhari12The Australian and New Zealand NMO CollaborationFabienne Brilot13Fabienne Brilot14Patrick Waters15Simon A. Broadley16Simon A. Broadley17Pathology Queensland Central Laboratory, Division of Immunology, Royal Brisbane and Women's Hospital, Herston, QLD, AustraliaOxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomOxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomBrain Autoimmunity Group, Kids Neuroscience Centre, Kids Research at the Children's Hospital, Westmead, NSW, AustraliaBrain and Mind Centre, University of Sydney, Camperdown, NSW, AustraliaBrain and Mind Centre, University of Sydney, Camperdown, NSW, AustraliaSchool of Biomedical Science, Medicine, University of Western Australia, Nedlands, WA, AustraliaPathWest Laboratory Medicine, Department of Immunology, QEII Medical Centre, Nedlands, WA, AustraliaBrain and Mind Centre, University of Sydney, Camperdown, NSW, AustraliaDepartment of Neurology, Royal North Shore Hospital, Sydney, NSW, AustraliaBrain Autoimmunity Group, Kids Neuroscience Centre, Kids Research at the Children's Hospital, Westmead, NSW, AustraliaSchool of Medicine, Gold Coast Campus, Griffith University, Southport, QLD, AustraliaDepartment of Neurology, Gold Coast University Hospital, Southport, QLD, AustraliaBrain Autoimmunity Group, Kids Neuroscience Centre, Kids Research at the Children's Hospital, Westmead, NSW, AustraliaBrain and Mind Centre, University of Sydney, Camperdown, NSW, AustraliaOxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United KingdomSchool of Medicine, Gold Coast Campus, Griffith University, Southport, QLD, AustraliaDepartment of Neurology, Gold Coast University Hospital, Southport, QLD, AustraliaWe have compared five different assays for antibodies to aquaporin-4 in 181 cases of suspected Neuromyelitis optica spectrum disorders (NMOSD) and 253 controls to assess their relative utility. As part of a clinically-based survey of NMOSD in Australia and New Zealand, cases of suspected NMOSD were referred from 23 centers. Clinical details and magnetic imaging were reviewed and used to apply the 2015 IPND diagnostic criteria. In addition, 101 age- and sex-matched patients with multiple sclerosis were referred. Other inflammatory disease (n = 49) and healthy controls (n = 103) were also recruited. Samples from all participants were tested using tissue-based indirect immunofluorescence assays and a subset were tested using four additional ELISA and cell-based assays. Antibodies to myelin oligodendrocyte glycoprotein (MOG) were also assayed. All aquaporin-4 antibody assays proved to be highly specific. Sensitivities ranged from 60 to 94%, with cell-based assays having the highest sensitivity. Antibodies to MOG were detected in 8/79 (10%) of the residual suspected cases of NMOSD. Under the 2015 IPND diagnostic criteria for NMOSD, cell-based assays for aquaporin-4 are sensitive and highly specific, performing better than tissue-based and ELISA assays. A fixed cell-based assay showed near-identical results to a live-cell based assay. Antibodies to MOG account for only a small number of suspected cases.https://www.frontiersin.org/article/10.3389/fneur.2019.01028/fullneuromyelitis opticaautoantibodyaquaporinmyelin oligodendrocyte glycoproteinastrocytopathydemyelination |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kerri Prain Mark Woodhall Angela Vincent Sudarshini Ramanathan Sudarshini Ramanathan Michael H. Barnett Christine S. Bundell Christine S. Bundell John D. E. Parratt John D. E. Parratt Roger A. Silvestrini Wajih Bukhari Wajih Bukhari The Australian and New Zealand NMO Collaboration Fabienne Brilot Fabienne Brilot Patrick Waters Simon A. Broadley Simon A. Broadley |
spellingShingle |
Kerri Prain Mark Woodhall Angela Vincent Sudarshini Ramanathan Sudarshini Ramanathan Michael H. Barnett Christine S. Bundell Christine S. Bundell John D. E. Parratt John D. E. Parratt Roger A. Silvestrini Wajih Bukhari Wajih Bukhari The Australian and New Zealand NMO Collaboration Fabienne Brilot Fabienne Brilot Patrick Waters Simon A. Broadley Simon A. Broadley AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD Frontiers in Neurology neuromyelitis optica autoantibody aquaporin myelin oligodendrocyte glycoprotein astrocytopathy demyelination |
author_facet |
Kerri Prain Mark Woodhall Angela Vincent Sudarshini Ramanathan Sudarshini Ramanathan Michael H. Barnett Christine S. Bundell Christine S. Bundell John D. E. Parratt John D. E. Parratt Roger A. Silvestrini Wajih Bukhari Wajih Bukhari The Australian and New Zealand NMO Collaboration Fabienne Brilot Fabienne Brilot Patrick Waters Simon A. Broadley Simon A. Broadley |
author_sort |
Kerri Prain |
title |
AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD |
title_short |
AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD |
title_full |
AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD |
title_fullStr |
AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD |
title_full_unstemmed |
AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD |
title_sort |
aqp4 antibody assay sensitivity comparison in the era of the 2015 diagnostic criteria for nmosd |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neurology |
issn |
1664-2295 |
publishDate |
2019-10-01 |
description |
We have compared five different assays for antibodies to aquaporin-4 in 181 cases of suspected Neuromyelitis optica spectrum disorders (NMOSD) and 253 controls to assess their relative utility. As part of a clinically-based survey of NMOSD in Australia and New Zealand, cases of suspected NMOSD were referred from 23 centers. Clinical details and magnetic imaging were reviewed and used to apply the 2015 IPND diagnostic criteria. In addition, 101 age- and sex-matched patients with multiple sclerosis were referred. Other inflammatory disease (n = 49) and healthy controls (n = 103) were also recruited. Samples from all participants were tested using tissue-based indirect immunofluorescence assays and a subset were tested using four additional ELISA and cell-based assays. Antibodies to myelin oligodendrocyte glycoprotein (MOG) were also assayed. All aquaporin-4 antibody assays proved to be highly specific. Sensitivities ranged from 60 to 94%, with cell-based assays having the highest sensitivity. Antibodies to MOG were detected in 8/79 (10%) of the residual suspected cases of NMOSD. Under the 2015 IPND diagnostic criteria for NMOSD, cell-based assays for aquaporin-4 are sensitive and highly specific, performing better than tissue-based and ELISA assays. A fixed cell-based assay showed near-identical results to a live-cell based assay. Antibodies to MOG account for only a small number of suspected cases. |
topic |
neuromyelitis optica autoantibody aquaporin myelin oligodendrocyte glycoprotein astrocytopathy demyelination |
url |
https://www.frontiersin.org/article/10.3389/fneur.2019.01028/full |
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