Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

A case of melioidosis with a mycotic aneurysm is reported. The blood and tissue isolates were identified as three different species of Burkholderia using the automated identification systems, VITEK 2 and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The...

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Main Authors: Hee Ryeong Jang, Chung Won Lee, Soon Jung Ok, Min Ji Kim, Mi Ju Bae, Seunghwan Song, Jongyoun Yi, Kye-Hyung Kim
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:International Journal of Infectious Diseases
Subjects:
Burkholderia pseudomallei
Melioidosis
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
RNA sequence analysis
Online Access:http://www.sciencedirect.com/science/article/pii/S1201971215001794
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spelling doaj-cd9648416f6445e68287eec6b0cf06162020-11-24T21:17:16ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112015-09-0138C626410.1016/j.ijid.2015.07.012Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometryHee Ryeong Jang0Chung Won Lee1Soon Jung Ok2Min Ji Kim3Mi Ju Bae4Seunghwan Song5Jongyoun Yi6Kye-Hyung Kim7Department of Internal Medicine, Pusan National University Hospital, 179 Gudeok-ro, Seogu, Busan 602-739, Republic of KoreaDepartment of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, Seogu, Busan, Republic of KoreaDepartment of Laboratory Medicine, Pusan National University Hospital, Seogu, Busan, Republic of KoreaDepartment of Internal Medicine, Pusan National University Hospital, 179 Gudeok-ro, Seogu, Busan 602-739, Republic of KoreaDepartment of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, Seogu, Busan, Republic of KoreaDepartment of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, Seogu, Busan, Republic of KoreaDepartment of Laboratory Medicine, Pusan National University Hospital, Seogu, Busan, Republic of KoreaDepartment of Internal Medicine, Pusan National University Hospital, 179 Gudeok-ro, Seogu, Busan 602-739, Republic of KoreaA case of melioidosis with a mycotic aneurysm is reported. The blood and tissue isolates were identified as three different species of Burkholderia using the automated identification systems, VITEK 2 and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The isolate was confirmed as Burkholderia pseudomallei by 16S rRNA sequencing. The typical features of the Gram staining of colonies and 16S rRNA sequencing can be useful to identify B. pseudomallei.http://www.sciencedirect.com/science/article/pii/S1201971215001794Burkholderia pseudomalleiMelioidosisMatrix-assisted laser desorption/ionization time-of-flight mass spectrometryRNA sequence analysis
collection DOAJ
language English
format Article
sources DOAJ
author Hee Ryeong Jang
Chung Won Lee
Soon Jung Ok
Min Ji Kim
Mi Ju Bae
Seunghwan Song
Jongyoun Yi
Kye-Hyung Kim
spellingShingle Hee Ryeong Jang
Chung Won Lee
Soon Jung Ok
Min Ji Kim
Mi Ju Bae
Seunghwan Song
Jongyoun Yi
Kye-Hyung Kim
Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
International Journal of Infectious Diseases
Burkholderia pseudomallei
Melioidosis
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
RNA sequence analysis
author_facet Hee Ryeong Jang
Chung Won Lee
Soon Jung Ok
Min Ji Kim
Mi Ju Bae
Seunghwan Song
Jongyoun Yi
Kye-Hyung Kim
author_sort Hee Ryeong Jang
title Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_short Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_full Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_fullStr Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_full_unstemmed Melioidosis presenting as a mycotic aneurysm in a Korean patient, diagnosed by 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
title_sort melioidosis presenting as a mycotic aneurysm in a korean patient, diagnosed by 16s rrna sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
publisher Elsevier
series International Journal of Infectious Diseases
issn 1201-9712
1878-3511
publishDate 2015-09-01
description A case of melioidosis with a mycotic aneurysm is reported. The blood and tissue isolates were identified as three different species of Burkholderia using the automated identification systems, VITEK 2 and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The isolate was confirmed as Burkholderia pseudomallei by 16S rRNA sequencing. The typical features of the Gram staining of colonies and 16S rRNA sequencing can be useful to identify B. pseudomallei.
topic Burkholderia pseudomallei
Melioidosis
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
RNA sequence analysis
url http://www.sciencedirect.com/science/article/pii/S1201971215001794
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