Altered expression of a quality control protease in E. coli reshapes the in vivo mutational landscape of a model enzyme
Protein mutational landscapes are shaped by the cellular environment, but key factors and their quantitative effects are often unknown. Here we show that Lon, a quality control protease naturally absent in common E. coli expression strains, drastically reshapes the mutational landscape of the metabo...
Main Authors: | Samuel Thompson, Yang Zhang, Christine Ingle, Kimberly A Reynolds, Tanja Kortemme |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2020-07-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/53476 |
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