Serum and plasma vascular endothelial growth factor in healthy dogs
Vascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy...
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Universidade de São Paulo
2013-10-01
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doaj-cda79a7cd3a7475da5021bb2b1288a2d2020-11-25T03:29:26ZengUniversidade de São PauloBrazilian Journal of Veterinary Research and Animal Science1413-95961678-44562013-10-0150510.11606/issn.2318-3659.v50i5p359-36376979Serum and plasma vascular endothelial growth factor in healthy dogsGenilson Fernandes de QueirozMaria Luiza FranchiniAmérico Froes Garcez NetoJulia Maria MateraVascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs.http://www.revistas.usp.br/bjvras/article/view/79923AngiogenesisGrowth factorOrganic fluids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Genilson Fernandes de Queiroz Maria Luiza Franchini Américo Froes Garcez Neto Julia Maria Matera |
spellingShingle |
Genilson Fernandes de Queiroz Maria Luiza Franchini Américo Froes Garcez Neto Julia Maria Matera Serum and plasma vascular endothelial growth factor in healthy dogs Brazilian Journal of Veterinary Research and Animal Science Angiogenesis Growth factor Organic fluids |
author_facet |
Genilson Fernandes de Queiroz Maria Luiza Franchini Américo Froes Garcez Neto Julia Maria Matera |
author_sort |
Genilson Fernandes de Queiroz |
title |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_short |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_full |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_fullStr |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_full_unstemmed |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_sort |
serum and plasma vascular endothelial growth factor in healthy dogs |
publisher |
Universidade de São Paulo |
series |
Brazilian Journal of Veterinary Research and Animal Science |
issn |
1413-9596 1678-4456 |
publishDate |
2013-10-01 |
description |
Vascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs. |
topic |
Angiogenesis Growth factor Organic fluids |
url |
http://www.revistas.usp.br/bjvras/article/view/79923 |
work_keys_str_mv |
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