LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites

LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites

Abstract Background Ligation-mediated PCR protocols have diverse uses including the identification of integration sites of insertional mutagens, integrating vectors and naturally occurring mobile genetic elements. For approaches that employ NGS sequencing, the relative abundance of integrations with...

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Bibliographic Details
Main Authors: Joanna C. Dawes, Philip Webster, Barbara Iadarola, Claudia Garcia-Diaz, Marian Dore, Bruce J. Bolt, Hamlata Dewchand, Gopuraja Dharmalingam, Alex P. McLatchie, Jakub Kaczor, Juan J. Caceres, Alberto Paccanaro, Laurence Game, Simona Parrinello, Anthony G. Uren
Format: Article
Language:English
Published: BMC 2020-02-01
Series:Mobile DNA
Subjects:
Genomics
Genetics
Mutagenesis
Retroviruses
Integration site cloning
Online Access:https://doi.org/10.1186/s13100-020-0201-4

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