Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L.
<p>Cantharidin and its analog, endothall, are known to have phytotoxic effects and their mechanism of action involves the inhibition of phosphatases. Enzymes and antioxidant compounds act as barriers against phytotoxic compounds. Catalase and peroxidase are among the most important antioxidant...
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University of Ljubljana, Biotechnical Faculty
2020-09-01
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doaj-cdb61e62d92b4dcaa1969f98567caddc2020-11-25T02:35:49ZengUniversity of Ljubljana, Biotechnical FacultyActa Agriculturae Slovenica1581-91751854-19412020-09-01116118919710.14720/aas.2020.116.1.1232419Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L.Yalda GHOTBZADEH KERMANI0Ramazan Ali KHAVARI NEJAD1Homa MAHMOODZADEH2Khadijeh SHAHROKH ABADI3Science and Research University Of Tehran,Iran.Science and Research University Of Tehran,Iran.Mashhad branch, Islamic Azad University , Mashhad , Iran.Mashhad branch, Islamic Azad University , Mashhad , Iran.<p>Cantharidin and its analog, endothall, are known to have phytotoxic effects and their mechanism of action involves the inhibition of phosphatases. Enzymes and antioxidant compounds act as barriers against phytotoxic compounds. Catalase and peroxidase are among the most important antioxidant enzymes. <em>Cichorium intybus </em>L. has traditionally been used for its medicinal properties and contains various phytochemical and enzymatic compounds. The present study aimed to investigate the comparative effects of cantharidin and endothall with concentration of 2.5, 5.5 and 10 µg ml<sup>-1</sup> on the changes in the gene expression of catalase and glutathione peroxidase. Furthermore, we assess the activities of these enzymes in the shoots and roots of <em>Cichorium intybus</em> L.. According to the findings, the expression of catalase and glutathione peroxidase increased in the samples treated with cantharidin more than endothall compared to the controls in both shoot (the most significant is in cantharidin with 2.5 µg ml<sup>-1 </sup>concentration) and root samples (the most significant is in cantharidin with 5.5 µg ml<sup>-1 </sup>concentration). In addition, the activity of catalase and concentrations of cantharidin (2.5 µg ml<sup>-1</sup>) in shoot samples led to the more intense stimulation of catalase and glutathione peroxidase compared to root samples. We suggest that cantharidin and endothall have negative effect on expression and absorption of antioxidant enzymes.</p>http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/1232cantharidincatalasecichorium intybus l.endothallglutathione reroxidase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yalda GHOTBZADEH KERMANI Ramazan Ali KHAVARI NEJAD Homa MAHMOODZADEH Khadijeh SHAHROKH ABADI |
spellingShingle |
Yalda GHOTBZADEH KERMANI Ramazan Ali KHAVARI NEJAD Homa MAHMOODZADEH Khadijeh SHAHROKH ABADI Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L. Acta Agriculturae Slovenica cantharidin catalase cichorium intybus l. endothall glutathione reroxidase |
author_facet |
Yalda GHOTBZADEH KERMANI Ramazan Ali KHAVARI NEJAD Homa MAHMOODZADEH Khadijeh SHAHROKH ABADI |
author_sort |
Yalda GHOTBZADEH KERMANI |
title |
Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L. |
title_short |
Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L. |
title_full |
Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L. |
title_fullStr |
Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L. |
title_full_unstemmed |
Comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in Cichorium intybus L. |
title_sort |
comparative effects of cantharidin and endothall on gene expression and activity of antioxidant enzymes in cichorium intybus l. |
publisher |
University of Ljubljana, Biotechnical Faculty |
series |
Acta Agriculturae Slovenica |
issn |
1581-9175 1854-1941 |
publishDate |
2020-09-01 |
description |
<p>Cantharidin and its analog, endothall, are known to have phytotoxic effects and their mechanism of action involves the inhibition of phosphatases. Enzymes and antioxidant compounds act as barriers against phytotoxic compounds. Catalase and peroxidase are among the most important antioxidant enzymes. <em>Cichorium intybus </em>L. has traditionally been used for its medicinal properties and contains various phytochemical and enzymatic compounds. The present study aimed to investigate the comparative effects of cantharidin and endothall with concentration of 2.5, 5.5 and 10 µg ml<sup>-1</sup> on the changes in the gene expression of catalase and glutathione peroxidase. Furthermore, we assess the activities of these enzymes in the shoots and roots of <em>Cichorium intybus</em> L.. According to the findings, the expression of catalase and glutathione peroxidase increased in the samples treated with cantharidin more than endothall compared to the controls in both shoot (the most significant is in cantharidin with 2.5 µg ml<sup>-1 </sup>concentration) and root samples (the most significant is in cantharidin with 5.5 µg ml<sup>-1 </sup>concentration). In addition, the activity of catalase and concentrations of cantharidin (2.5 µg ml<sup>-1</sup>) in shoot samples led to the more intense stimulation of catalase and glutathione peroxidase compared to root samples. We suggest that cantharidin and endothall have negative effect on expression and absorption of antioxidant enzymes.</p> |
topic |
cantharidin catalase cichorium intybus l. endothall glutathione reroxidase |
url |
http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/1232 |
work_keys_str_mv |
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