Black Soldier Fly, <em>Hermetia illucens</em> as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, <em>Lates calcarifer</em> (Bloch, 1790)
A feeding trial was conducted to test the effects of partial replacement of fishmeal (FM) protein and fish oil (FO) with partially defatted black soldier fly, <i>Hermetia illucens</i> insect protein, and oil, respectively, on growth performance, immune response, gut and skin barrier stat...
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doaj-cdce92244d5f40e2a959005bdcc6e3522021-06-30T23:28:49ZengMDPI AGBiology2079-77372021-06-011050550510.3390/biology10060505Black Soldier Fly, <em>Hermetia illucens</em> as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, <em>Lates calcarifer</em> (Bloch, 1790)Amanda Hender0Muhammad A.B. Siddik1Janet Howieson2Ravi Fotedar3School of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bentley, WA 6102, AustraliaSchool of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bentley, WA 6102, AustraliaSchool of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bentley, WA 6102, AustraliaSchool of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bentley, WA 6102, AustraliaA feeding trial was conducted to test the effects of partial replacement of fishmeal (FM) protein and fish oil (FO) with partially defatted black soldier fly, <i>Hermetia illucens</i> insect protein, and oil, respectively, on growth performance, immune response, gut and skin barrier status, and flesh quality in juvenile barramundi. Four isonitrogenous and isocaloric diets used in the study were a control diet based on FM, 30% FM replaced with <i>H. illucens</i> protein (HiP), 30% FO replaced with <i>H. illucens</i> oil (HiO), and both 30% FM and 30% FO replaced with <i>H. illucens</i> protein and oil (HiPO). Diets were fed twice a day to satiety in triplicated groups of barramundi with an initial body weight of 1.74 ± 0.15 g per fish. At the end of the trial, growth and feed utilization indices were found insignificant (<i>p</i> > 0.05) between the test diets and control. A significant increase in bactericidal activity was observed in fish fed the HiP diet while serum lysozyme activity was unchanged. Stress-related heat shock proteins (HSP70 and HSP90) did not differ significantly among the test diets while immune-relevant genes (IL-1β and IL-10) were significantly upregulated in HiP and HiOP groups. The number of mucin cells were increased in the gut and skin of HiP and HiOP fed fish when compared to the control diet. The total fatty acid compositions (∑SFA, ∑MUFA, ∑PUFA, ∑n-3, and ∑n-6) in the muscles of barramundi were not significantly influenced with <i>H. illucens</i> protein and oil diets when compared to the control.https://www.mdpi.com/2079-7737/10/6/505Insect larvaefishmeal replacementintestinal morphologyacidic mucinfatty acidcytokine expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amanda Hender Muhammad A.B. Siddik Janet Howieson Ravi Fotedar |
spellingShingle |
Amanda Hender Muhammad A.B. Siddik Janet Howieson Ravi Fotedar Black Soldier Fly, <em>Hermetia illucens</em> as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, <em>Lates calcarifer</em> (Bloch, 1790) Biology Insect larvae fishmeal replacement intestinal morphology acidic mucin fatty acid cytokine expression |
author_facet |
Amanda Hender Muhammad A.B. Siddik Janet Howieson Ravi Fotedar |
author_sort |
Amanda Hender |
title |
Black Soldier Fly, <em>Hermetia illucens</em> as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, <em>Lates calcarifer</em> (Bloch, 1790) |
title_short |
Black Soldier Fly, <em>Hermetia illucens</em> as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, <em>Lates calcarifer</em> (Bloch, 1790) |
title_full |
Black Soldier Fly, <em>Hermetia illucens</em> as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, <em>Lates calcarifer</em> (Bloch, 1790) |
title_fullStr |
Black Soldier Fly, <em>Hermetia illucens</em> as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, <em>Lates calcarifer</em> (Bloch, 1790) |
title_full_unstemmed |
Black Soldier Fly, <em>Hermetia illucens</em> as an Alternative to Fishmeal Protein and Fish Oil: Impact on Growth, Immune Response, Mucosal Barrier Status, and Flesh Quality of Juvenile Barramundi, <em>Lates calcarifer</em> (Bloch, 1790) |
title_sort |
black soldier fly, <em>hermetia illucens</em> as an alternative to fishmeal protein and fish oil: impact on growth, immune response, mucosal barrier status, and flesh quality of juvenile barramundi, <em>lates calcarifer</em> (bloch, 1790) |
publisher |
MDPI AG |
series |
Biology |
issn |
2079-7737 |
publishDate |
2021-06-01 |
description |
A feeding trial was conducted to test the effects of partial replacement of fishmeal (FM) protein and fish oil (FO) with partially defatted black soldier fly, <i>Hermetia illucens</i> insect protein, and oil, respectively, on growth performance, immune response, gut and skin barrier status, and flesh quality in juvenile barramundi. Four isonitrogenous and isocaloric diets used in the study were a control diet based on FM, 30% FM replaced with <i>H. illucens</i> protein (HiP), 30% FO replaced with <i>H. illucens</i> oil (HiO), and both 30% FM and 30% FO replaced with <i>H. illucens</i> protein and oil (HiPO). Diets were fed twice a day to satiety in triplicated groups of barramundi with an initial body weight of 1.74 ± 0.15 g per fish. At the end of the trial, growth and feed utilization indices were found insignificant (<i>p</i> > 0.05) between the test diets and control. A significant increase in bactericidal activity was observed in fish fed the HiP diet while serum lysozyme activity was unchanged. Stress-related heat shock proteins (HSP70 and HSP90) did not differ significantly among the test diets while immune-relevant genes (IL-1β and IL-10) were significantly upregulated in HiP and HiOP groups. The number of mucin cells were increased in the gut and skin of HiP and HiOP fed fish when compared to the control diet. The total fatty acid compositions (∑SFA, ∑MUFA, ∑PUFA, ∑n-3, and ∑n-6) in the muscles of barramundi were not significantly influenced with <i>H. illucens</i> protein and oil diets when compared to the control. |
topic |
Insect larvae fishmeal replacement intestinal morphology acidic mucin fatty acid cytokine expression |
url |
https://www.mdpi.com/2079-7737/10/6/505 |
work_keys_str_mv |
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