Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance
Digitally driven manufacturing technologies such as aerosol jet printing (AJP) can make a significant contribution to enabling new capabilities in the field of tissue engineering disease modeling and drug screening. AJP is an emerging non-contact and mask-less printing process which has distinct adv...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-08-01
|
Series: | Frontiers in Cell and Developmental Biology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.722294/full |
id |
doaj-cdd40ce01416400d8852320e929a3279 |
---|---|
record_format |
Article |
spelling |
doaj-cdd40ce01416400d8852320e929a32792021-09-03T16:27:46ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-08-01910.3389/fcell.2021.722294722294Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal GuidanceAndrew J. Capel0Matthew A. A. Smith1Silvia Taccola2Maria Pardo-Figuerez3Rowan P. Rimington4Mark P. Lewis5Steven D. R. Christie6Robert W. Kay7Russell A. Harris8School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, United KingdomFaculty of Engineering and Physical Sciences, School of Mechanical Engineering, University of Leeds, Leeds, United KingdomFaculty of Engineering and Physical Sciences, School of Mechanical Engineering, University of Leeds, Leeds, United KingdomSchool of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, United KingdomSchool of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, United KingdomSchool of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, United KingdomSchool of Science, Loughborough University, Loughborough, United KingdomFaculty of Engineering and Physical Sciences, School of Mechanical Engineering, University of Leeds, Leeds, United KingdomFaculty of Engineering and Physical Sciences, School of Mechanical Engineering, University of Leeds, Leeds, United KingdomDigitally driven manufacturing technologies such as aerosol jet printing (AJP) can make a significant contribution to enabling new capabilities in the field of tissue engineering disease modeling and drug screening. AJP is an emerging non-contact and mask-less printing process which has distinct advantages over other patterning technologies as it offers versatile, high-resolution, direct-write deposition of a variety of materials on planar and non-planar surfaces. This research demonstrates the ability of AJP to print digitally controlled patterns that influence neuronal guidance. These consist of patterned poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) tracks on both glass and poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) coated glass surfaces, promoting selective adhesion of SH-SY5Y neuroblastoma cells. The cell attractive patterns had a maximum height ≥0.2 μm, width and half height ≥15 μm, Ra = 3.5 nm, and RMS = 4.1. The developed biocompatible PEDOT:PSS ink was shown to promote adhesion, growth and differentiation of SH-SY5Y neuronal cells. SH-SY5Y cells cultured directly onto these features exhibited increased nuclei and neuronal alignment on both substrates. In addition, the cell adhesion to the substrate was selective when cultured onto the PKSPMA surfaces resulting in a highly organized neural pattern. This demonstrated the ability to rapidly and flexibly realize intricate and accurate cell patterns by a computer controlled process.https://www.frontiersin.org/articles/10.3389/fcell.2021.722294/fulldirect writeaerosol jet printingmicrofabricationneuronal alignmenttissue engineeringbiomaterials |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrew J. Capel Matthew A. A. Smith Silvia Taccola Maria Pardo-Figuerez Rowan P. Rimington Mark P. Lewis Steven D. R. Christie Robert W. Kay Russell A. Harris |
spellingShingle |
Andrew J. Capel Matthew A. A. Smith Silvia Taccola Maria Pardo-Figuerez Rowan P. Rimington Mark P. Lewis Steven D. R. Christie Robert W. Kay Russell A. Harris Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance Frontiers in Cell and Developmental Biology direct write aerosol jet printing microfabrication neuronal alignment tissue engineering biomaterials |
author_facet |
Andrew J. Capel Matthew A. A. Smith Silvia Taccola Maria Pardo-Figuerez Rowan P. Rimington Mark P. Lewis Steven D. R. Christie Robert W. Kay Russell A. Harris |
author_sort |
Andrew J. Capel |
title |
Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance |
title_short |
Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance |
title_full |
Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance |
title_fullStr |
Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance |
title_full_unstemmed |
Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance |
title_sort |
digitally driven aerosol jet printing to enable customisable neuronal guidance |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-08-01 |
description |
Digitally driven manufacturing technologies such as aerosol jet printing (AJP) can make a significant contribution to enabling new capabilities in the field of tissue engineering disease modeling and drug screening. AJP is an emerging non-contact and mask-less printing process which has distinct advantages over other patterning technologies as it offers versatile, high-resolution, direct-write deposition of a variety of materials on planar and non-planar surfaces. This research demonstrates the ability of AJP to print digitally controlled patterns that influence neuronal guidance. These consist of patterned poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) tracks on both glass and poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) coated glass surfaces, promoting selective adhesion of SH-SY5Y neuroblastoma cells. The cell attractive patterns had a maximum height ≥0.2 μm, width and half height ≥15 μm, Ra = 3.5 nm, and RMS = 4.1. The developed biocompatible PEDOT:PSS ink was shown to promote adhesion, growth and differentiation of SH-SY5Y neuronal cells. SH-SY5Y cells cultured directly onto these features exhibited increased nuclei and neuronal alignment on both substrates. In addition, the cell adhesion to the substrate was selective when cultured onto the PKSPMA surfaces resulting in a highly organized neural pattern. This demonstrated the ability to rapidly and flexibly realize intricate and accurate cell patterns by a computer controlled process. |
topic |
direct write aerosol jet printing microfabrication neuronal alignment tissue engineering biomaterials |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.722294/full |
work_keys_str_mv |
AT andrewjcapel digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance AT matthewaasmith digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance AT silviataccola digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance AT mariapardofiguerez digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance AT rowanprimington digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance AT markplewis digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance AT stevendrchristie digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance AT robertwkay digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance AT russellaharris digitallydrivenaerosoljetprintingtoenablecustomisableneuronalguidance |
_version_ |
1717816202328276992 |