The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes Mellitus

Diabetes is a disorder in the metabolism of carbohydrates, lipids and proteins with various causes. From the previous research, GPR43 is a potent clinical target for the treatment of type 2 diabetes. It can be activated by Short Chain Fatty Acid (SCFA) which is an organic fatty acid produced through...

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Main Authors: Rizky Amalia, Karyono Mintaroem, Loeki Enggar Fitri, Nashi Widodo, Hidayat Sujuti
Format: Article
Language:English
Published: University of Brawijaya 2019-05-01
Series:Research Journal of Life Science
Subjects:
Online Access:https://rjls.ub.ac.id/index.php/rjls/article/view/242
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spelling doaj-cde326c1a21b499784f70ad8bd8024be2020-11-24T20:51:23ZengUniversity of BrawijayaResearch Journal of Life Science2355-99262019-05-0161465310.21776/ub.rjls.2019.006.01.6129The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes MellitusRizky Amalia0Karyono Mintaroem1Loeki Enggar Fitri2Nashi Widodo3Hidayat Sujuti4Master Program in Biomedical Science, Faculty of Medicine, University of BrawijayaDepartment of Pathology Anatomy, Faculty of Medicine, University of BrawijayaDepartment of Parasitology, Faculty of Medicine, University of BrawijayaDepartment of Biology, Faculty of Mathematics and Natural Science, University of BrawijayaDepartment of Biochemistry, Faculty of Medicine, University of BrawijayaDiabetes is a disorder in the metabolism of carbohydrates, lipids and proteins with various causes. From the previous research, GPR43 is a potent clinical target for the treatment of type 2 diabetes. It can be activated by Short Chain Fatty Acid (SCFA) which is an organic fatty acid produced through fermentation of dietary fiber by bacteria in the distal intestine. The aim of this study was to analyze the effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) in silico. The study used 8 sequences of GPR43: ID AF024690, AC002511, EU432114, BC095535, BC096198, BC096199, BC096200, BC096201 and  data of ligand (SCFA): acetic acid (CID 176), Butyric acid (CID 264) and Propionic acid (CID 1032). GPR43 was modelled using I-TASSER, Sequence and structural alignments were conducted using Bioedit and Superpose V.10, respectively. The Docking process was done using PyRx and molecular interaction was analyzed using Discovery Studio 2016. The Result showed that three types of SCFA bind to GPR43 variants T and GPR43 variant M obtained a similar pattern. The binding affinity from the largest to the smallest is butyric acid > propionic acid > acetic acid. GPR43 variant T has greater affinity to the SCFA than the GPR43 variant M. There is no differences of preference between GPR43 variant T and GPR43 variant M to bind SCFA.https://rjls.ub.ac.id/index.php/rjls/article/view/242GPR43SCFAacetic acidpropionic acidbutyric acid.
collection DOAJ
language English
format Article
sources DOAJ
author Rizky Amalia
Karyono Mintaroem
Loeki Enggar Fitri
Nashi Widodo
Hidayat Sujuti
spellingShingle Rizky Amalia
Karyono Mintaroem
Loeki Enggar Fitri
Nashi Widodo
Hidayat Sujuti
The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes Mellitus
Research Journal of Life Science
GPR43
SCFA
acetic acid
propionic acid
butyric acid.
author_facet Rizky Amalia
Karyono Mintaroem
Loeki Enggar Fitri
Nashi Widodo
Hidayat Sujuti
author_sort Rizky Amalia
title The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes Mellitus
title_short The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes Mellitus
title_full The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes Mellitus
title_fullStr The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes Mellitus
title_full_unstemmed The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes Mellitus
title_sort effect of c680t gpr43 gene variations to its interaction with short chain fatty acid (scfa) in type 2 diabetes mellitus
publisher University of Brawijaya
series Research Journal of Life Science
issn 2355-9926
publishDate 2019-05-01
description Diabetes is a disorder in the metabolism of carbohydrates, lipids and proteins with various causes. From the previous research, GPR43 is a potent clinical target for the treatment of type 2 diabetes. It can be activated by Short Chain Fatty Acid (SCFA) which is an organic fatty acid produced through fermentation of dietary fiber by bacteria in the distal intestine. The aim of this study was to analyze the effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) in silico. The study used 8 sequences of GPR43: ID AF024690, AC002511, EU432114, BC095535, BC096198, BC096199, BC096200, BC096201 and  data of ligand (SCFA): acetic acid (CID 176), Butyric acid (CID 264) and Propionic acid (CID 1032). GPR43 was modelled using I-TASSER, Sequence and structural alignments were conducted using Bioedit and Superpose V.10, respectively. The Docking process was done using PyRx and molecular interaction was analyzed using Discovery Studio 2016. The Result showed that three types of SCFA bind to GPR43 variants T and GPR43 variant M obtained a similar pattern. The binding affinity from the largest to the smallest is butyric acid > propionic acid > acetic acid. GPR43 variant T has greater affinity to the SCFA than the GPR43 variant M. There is no differences of preference between GPR43 variant T and GPR43 variant M to bind SCFA.
topic GPR43
SCFA
acetic acid
propionic acid
butyric acid.
url https://rjls.ub.ac.id/index.php/rjls/article/view/242
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