Genotoxicity of <em>Euphorbia hirta</em>: An <em>Allium cepa</em> Assay
The potential genotoxic effects of methanolic extracts of <em>Euphorbia hirta </em>which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using <em>Allium cep...
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doaj-ce0f2d8e524641b4a39476809293a7672020-11-24T23:41:40ZengMDPI AGMolecules1420-30492012-06-011777782779110.3390/molecules17077782Genotoxicity of <em>Euphorbia hirta</em>: An <em>Allium cepa</em> AssayKwan Yuet PingIbrahim DarahUmi Kalsom YusufChen YengSreenivasan SasidharanThe potential genotoxic effects of methanolic extracts of <em>Euphorbia hirta </em>which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using <em>Allium cepa</em> assay. The extracts of 125, 250, 500 and 1,000 µg/mL were tested on root meristems of <em>A. cepa</em>. Ethylmethanesulfonate was used as positive control and distilled water was used as negative control. The result showed that mitotic index decreased as the concentrations of <em>E. hirta</em> extract increased. A dose-dependent increase of chromosome aberrations was also observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Micronucleated cells were also observed at interphase. Result of this study confirmed that the methanol extracts of <em>E. hirta </em>exerted significant genotoxic and mitodepressive effects at 1,000 µg/mL.http://www.mdpi.com/1420-3049/17/7/7782genotoxicity<em>Allium cepa</em><em> </em>ethylmethane sulfonatemitotic indexchromosome aberrations |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kwan Yuet Ping Ibrahim Darah Umi Kalsom Yusuf Chen Yeng Sreenivasan Sasidharan |
spellingShingle |
Kwan Yuet Ping Ibrahim Darah Umi Kalsom Yusuf Chen Yeng Sreenivasan Sasidharan Genotoxicity of <em>Euphorbia hirta</em>: An <em>Allium cepa</em> Assay Molecules genotoxicity <em>Allium cepa</em> <em> </em>ethylmethane sulfonate mitotic index chromosome aberrations |
author_facet |
Kwan Yuet Ping Ibrahim Darah Umi Kalsom Yusuf Chen Yeng Sreenivasan Sasidharan |
author_sort |
Kwan Yuet Ping |
title |
Genotoxicity of <em>Euphorbia hirta</em>: An <em>Allium cepa</em> Assay |
title_short |
Genotoxicity of <em>Euphorbia hirta</em>: An <em>Allium cepa</em> Assay |
title_full |
Genotoxicity of <em>Euphorbia hirta</em>: An <em>Allium cepa</em> Assay |
title_fullStr |
Genotoxicity of <em>Euphorbia hirta</em>: An <em>Allium cepa</em> Assay |
title_full_unstemmed |
Genotoxicity of <em>Euphorbia hirta</em>: An <em>Allium cepa</em> Assay |
title_sort |
genotoxicity of <em>euphorbia hirta</em>: an <em>allium cepa</em> assay |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2012-06-01 |
description |
The potential genotoxic effects of methanolic extracts of <em>Euphorbia hirta </em>which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using <em>Allium cepa</em> assay. The extracts of 125, 250, 500 and 1,000 µg/mL were tested on root meristems of <em>A. cepa</em>. Ethylmethanesulfonate was used as positive control and distilled water was used as negative control. The result showed that mitotic index decreased as the concentrations of <em>E. hirta</em> extract increased. A dose-dependent increase of chromosome aberrations was also observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Micronucleated cells were also observed at interphase. Result of this study confirmed that the methanol extracts of <em>E. hirta </em>exerted significant genotoxic and mitodepressive effects at 1,000 µg/mL. |
topic |
genotoxicity <em>Allium cepa</em> <em> </em>ethylmethane sulfonate mitotic index chromosome aberrations |
url |
http://www.mdpi.com/1420-3049/17/7/7782 |
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