Single‐cell imaging of genome organization and dynamics
Abstract Probing the architecture, mechanism, and dynamics of genome folding is fundamental to our understanding of genome function in homeostasis and disease. Most chromosome conformation capture studies dissect the genome architecture with population‐ and time‐averaged snapshots and thus have limi...
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Online Access: | https://doi.org/10.15252/msb.20209653 |
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doaj-ce170cab2ef0464aaf9784f83beac8992021-07-29T07:30:28ZengWileyMolecular Systems Biology1744-42922021-07-01177n/an/a10.15252/msb.20209653Single‐cell imaging of genome organization and dynamicsLiangqi Xie0Zhe Liu1Janelia Research Campus Howard Hughes Medical Institute Ashburn VA USAJanelia Research Campus Howard Hughes Medical Institute Ashburn VA USAAbstract Probing the architecture, mechanism, and dynamics of genome folding is fundamental to our understanding of genome function in homeostasis and disease. Most chromosome conformation capture studies dissect the genome architecture with population‐ and time‐averaged snapshots and thus have limited capabilities to reveal 3D nuclear organization and dynamics at the single‐cell level. Here, we discuss emerging imaging techniques ranging from light microscopy to electron microscopy that enable investigation of genome folding and dynamics at high spatial and temporal resolution. Results from these studies complement genomic data, unveiling principles underlying the spatial arrangement of the genome and its potential functional links to diverse biological activities in the nucleus.https://doi.org/10.15252/msb.20209653chromatin dynamicsgenome organizationimagingsingle cellsuper‐resolution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liangqi Xie Zhe Liu |
spellingShingle |
Liangqi Xie Zhe Liu Single‐cell imaging of genome organization and dynamics Molecular Systems Biology chromatin dynamics genome organization imaging single cell super‐resolution |
author_facet |
Liangqi Xie Zhe Liu |
author_sort |
Liangqi Xie |
title |
Single‐cell imaging of genome organization and dynamics |
title_short |
Single‐cell imaging of genome organization and dynamics |
title_full |
Single‐cell imaging of genome organization and dynamics |
title_fullStr |
Single‐cell imaging of genome organization and dynamics |
title_full_unstemmed |
Single‐cell imaging of genome organization and dynamics |
title_sort |
single‐cell imaging of genome organization and dynamics |
publisher |
Wiley |
series |
Molecular Systems Biology |
issn |
1744-4292 |
publishDate |
2021-07-01 |
description |
Abstract Probing the architecture, mechanism, and dynamics of genome folding is fundamental to our understanding of genome function in homeostasis and disease. Most chromosome conformation capture studies dissect the genome architecture with population‐ and time‐averaged snapshots and thus have limited capabilities to reveal 3D nuclear organization and dynamics at the single‐cell level. Here, we discuss emerging imaging techniques ranging from light microscopy to electron microscopy that enable investigation of genome folding and dynamics at high spatial and temporal resolution. Results from these studies complement genomic data, unveiling principles underlying the spatial arrangement of the genome and its potential functional links to diverse biological activities in the nucleus. |
topic |
chromatin dynamics genome organization imaging single cell super‐resolution |
url |
https://doi.org/10.15252/msb.20209653 |
work_keys_str_mv |
AT liangqixie singlecellimagingofgenomeorganizationanddynamics AT zheliu singlecellimagingofgenomeorganizationanddynamics |
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1721259307258871808 |