Fetal lung C4BPA induces p100 processing in human placenta
Abstract The non-canonical NF-κB signaling may be a central integrator of a placental clock that governs the length of human pregnancy. We sought to identify fetal signals that could activate this NF-κB pathway in the placenta, and in turn, contribute to the onset of labor. Proteomics analysis of ex...
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doaj-ce25bda40c1c46b8a4cb3b799bb23b122020-12-08T06:35:06ZengNature Publishing GroupScientific Reports2045-23222019-04-019111010.1038/s41598-019-42078-0Fetal lung C4BPA induces p100 processing in human placentaMayra Cruz Ithier0Nataliya Parobchak1Stacy Yadava2Jerry Cheng3Bingbing Wang4Todd Rosen5Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Rutgers Robert Wood Johnson Medical SchoolDepartment of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Rutgers Robert Wood Johnson Medical SchoolDepartment of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Rutgers Robert Wood Johnson Medical SchoolThe Cardiovascular Institute of New Jersey, Rutgers Robert Wood Johnson Medical SchoolDepartment of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Rutgers Robert Wood Johnson Medical SchoolDepartment of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Rutgers Robert Wood Johnson Medical SchoolAbstract The non-canonical NF-κB signaling may be a central integrator of a placental clock that governs the length of human pregnancy. We sought to identify fetal signals that could activate this NF-κB pathway in the placenta, and in turn, contribute to the onset of labor. Proteomics analysis of exosomes purified from fetal cord arterial blood revealed a total of 328 proteins, among which 48 were more significantly abundant (p < 0.01) in samples from women who delivered following elective Cesarean-section at term (39 to 40 weeks of estimated gestational age, EGA) compared to those who had elective Cesarean deliveries near term (35 to 36 weeks of EGA). Computational, crystal structural, and gene functional analyses showed that one of these 48 proteins, C4BPA, binds to CD40 of placental villous trophoblast to activate p100 processing to p52, and in turn, pro-labor genes. These results suggest that fetal C4BPA-induced activation of non-canonical NF-κB in human placenta may play a critical role in processes of term or preterm labor.https://doi.org/10.1038/s41598-019-42078-0 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mayra Cruz Ithier Nataliya Parobchak Stacy Yadava Jerry Cheng Bingbing Wang Todd Rosen |
spellingShingle |
Mayra Cruz Ithier Nataliya Parobchak Stacy Yadava Jerry Cheng Bingbing Wang Todd Rosen Fetal lung C4BPA induces p100 processing in human placenta Scientific Reports |
author_facet |
Mayra Cruz Ithier Nataliya Parobchak Stacy Yadava Jerry Cheng Bingbing Wang Todd Rosen |
author_sort |
Mayra Cruz Ithier |
title |
Fetal lung C4BPA induces p100 processing in human placenta |
title_short |
Fetal lung C4BPA induces p100 processing in human placenta |
title_full |
Fetal lung C4BPA induces p100 processing in human placenta |
title_fullStr |
Fetal lung C4BPA induces p100 processing in human placenta |
title_full_unstemmed |
Fetal lung C4BPA induces p100 processing in human placenta |
title_sort |
fetal lung c4bpa induces p100 processing in human placenta |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2019-04-01 |
description |
Abstract The non-canonical NF-κB signaling may be a central integrator of a placental clock that governs the length of human pregnancy. We sought to identify fetal signals that could activate this NF-κB pathway in the placenta, and in turn, contribute to the onset of labor. Proteomics analysis of exosomes purified from fetal cord arterial blood revealed a total of 328 proteins, among which 48 were more significantly abundant (p < 0.01) in samples from women who delivered following elective Cesarean-section at term (39 to 40 weeks of estimated gestational age, EGA) compared to those who had elective Cesarean deliveries near term (35 to 36 weeks of EGA). Computational, crystal structural, and gene functional analyses showed that one of these 48 proteins, C4BPA, binds to CD40 of placental villous trophoblast to activate p100 processing to p52, and in turn, pro-labor genes. These results suggest that fetal C4BPA-induced activation of non-canonical NF-κB in human placenta may play a critical role in processes of term or preterm labor. |
url |
https://doi.org/10.1038/s41598-019-42078-0 |
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