Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea
This study aimed to describe the distribution and characterization of fecal extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli isolates from healthy companion animals and cohabiting humans. A total of 968 rectal swab samples from 340 participants, including healthy companion a...
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doaj-ce4317bc9caf4ad69d4584b23707c90b2020-11-25T02:03:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-04-011110.3389/fmicb.2020.00674535641Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South KoreaJun Sung Hong0Wonkeun Song1Hee-Myung Park2Jae-Young Oh3Jong-Chan Chae4Seri Jeong5Seok Hoon Jeong6Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South KoreaDepartment of Laboratory Medicine, College of Medicine, Hallym University, Chuncheon, South KoreaDepartment of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, South KoreaDepartment of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, South KoreaDivision of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South KoreaDepartment of Laboratory Medicine, College of Medicine, Hallym University, Chuncheon, South KoreaDepartment of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South KoreaThis study aimed to describe the distribution and characterization of fecal extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli isolates from healthy companion animals and cohabiting humans. A total of 968 rectal swab samples from 340 participants, including healthy companion animals and cohabiting humans, were collected from 130 households in South Korea from 2018 to 2019. To determine the bacterial profiles of the participants, several experiments were performed as follows: antimicrobial susceptibility testing, PCR and direct sequencing for ESBL/AmpC production, PFGE, MLST, whole genome sequencing and qRT-PCR. A total of 24.9 and 21.5% of the E. coli isolates from healthy companion animals and cohabiting humans were ESBL/AmpC producers, respectively. The blaCTX–M–14 gene was the most prevalent ESC resistance gene in both pets (n = 25/95, 26.3%) and humans (n = 44/126, 34.9%). The blaCMY–2 gene was also largely detected in pets (n = 19, 20.0%). Overall, intrahousehold pet-human sharing of ESBL/AmpC E. coli isolates occurred in 4.8% of households, and the isolates were all CTX-M-14 producers. In particular, ten CMY-2-producing E. coli isolates from seven dogs and three humans in the different households belonged to the same pulsotype. The MIC values of cefoxitin and the transcription level in CMY-2-producing E. coli isolates were proportional to the blaCMY–2 copy number on the chromosome. Our results showed that the clonal spread of fecal ESBL/AmpC-producing E. coli households’ isolates between healthy companion animals and cohabiting humans was rare, but it could happen. In particular, E. coli ST405 isolates carrying multiple blaCMY–2 genes on the chromosome was sporadically spread between companion animals and humans in South Korea.https://www.frontiersin.org/article/10.3389/fmicb.2020.00674/fullEscherichia coliCTX-MCMY-2healthy companion animalhumancopy number |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun Sung Hong Wonkeun Song Hee-Myung Park Jae-Young Oh Jong-Chan Chae Seri Jeong Seok Hoon Jeong |
spellingShingle |
Jun Sung Hong Wonkeun Song Hee-Myung Park Jae-Young Oh Jong-Chan Chae Seri Jeong Seok Hoon Jeong Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea Frontiers in Microbiology Escherichia coli CTX-M CMY-2 healthy companion animal human copy number |
author_facet |
Jun Sung Hong Wonkeun Song Hee-Myung Park Jae-Young Oh Jong-Chan Chae Seri Jeong Seok Hoon Jeong |
author_sort |
Jun Sung Hong |
title |
Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea |
title_short |
Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea |
title_full |
Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea |
title_fullStr |
Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea |
title_full_unstemmed |
Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea |
title_sort |
molecular characterization of fecal extended-spectrum β-lactamase- and ampc β-lactamase-producing escherichia coli from healthy companion animals and cohabiting humans in south korea |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-04-01 |
description |
This study aimed to describe the distribution and characterization of fecal extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli isolates from healthy companion animals and cohabiting humans. A total of 968 rectal swab samples from 340 participants, including healthy companion animals and cohabiting humans, were collected from 130 households in South Korea from 2018 to 2019. To determine the bacterial profiles of the participants, several experiments were performed as follows: antimicrobial susceptibility testing, PCR and direct sequencing for ESBL/AmpC production, PFGE, MLST, whole genome sequencing and qRT-PCR. A total of 24.9 and 21.5% of the E. coli isolates from healthy companion animals and cohabiting humans were ESBL/AmpC producers, respectively. The blaCTX–M–14 gene was the most prevalent ESC resistance gene in both pets (n = 25/95, 26.3%) and humans (n = 44/126, 34.9%). The blaCMY–2 gene was also largely detected in pets (n = 19, 20.0%). Overall, intrahousehold pet-human sharing of ESBL/AmpC E. coli isolates occurred in 4.8% of households, and the isolates were all CTX-M-14 producers. In particular, ten CMY-2-producing E. coli isolates from seven dogs and three humans in the different households belonged to the same pulsotype. The MIC values of cefoxitin and the transcription level in CMY-2-producing E. coli isolates were proportional to the blaCMY–2 copy number on the chromosome. Our results showed that the clonal spread of fecal ESBL/AmpC-producing E. coli households’ isolates between healthy companion animals and cohabiting humans was rare, but it could happen. In particular, E. coli ST405 isolates carrying multiple blaCMY–2 genes on the chromosome was sporadically spread between companion animals and humans in South Korea. |
topic |
Escherichia coli CTX-M CMY-2 healthy companion animal human copy number |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00674/full |
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