LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES
ABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, e...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade de Investigações Florestais
2019-02-01
|
Series: | Revista Árvore |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622018000600203&lng=en&tlng=en |
id |
doaj-ce5f5e44d0e841a3aa64cb127a69c066 |
---|---|
record_format |
Article |
spelling |
doaj-ce5f5e44d0e841a3aa64cb127a69c0662020-11-24T22:17:45ZengSociedade de Investigações FlorestaisRevista Árvore1806-90882019-02-0142610.1590/1806-90882018000600004S0100-67622018000600203LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONESDenys Matheus Santana Costa SouzaAloisio XavierWagner Campos OtoniNatane Amaral MirandaJoane Helena MaggioniABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622018000600203&lng=en&tlng=enIn vitro propagationVegetative propagationLEDs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Denys Matheus Santana Costa Souza Aloisio Xavier Wagner Campos Otoni Natane Amaral Miranda Joane Helena Maggioni |
spellingShingle |
Denys Matheus Santana Costa Souza Aloisio Xavier Wagner Campos Otoni Natane Amaral Miranda Joane Helena Maggioni LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES Revista Árvore In vitro propagation Vegetative propagation LEDs |
author_facet |
Denys Matheus Santana Costa Souza Aloisio Xavier Wagner Campos Otoni Natane Amaral Miranda Joane Helena Maggioni |
author_sort |
Denys Matheus Santana Costa Souza |
title |
LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES |
title_short |
LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES |
title_full |
LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES |
title_fullStr |
LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES |
title_full_unstemmed |
LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES |
title_sort |
light quality in the in vitro introduction of corymbia hybrid clones |
publisher |
Sociedade de Investigações Florestais |
series |
Revista Árvore |
issn |
1806-9088 |
publishDate |
2019-02-01 |
description |
ABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction. |
topic |
In vitro propagation Vegetative propagation LEDs |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622018000600203&lng=en&tlng=en |
work_keys_str_mv |
AT denysmatheussantanacostasouza lightqualityintheinvitrointroductionofcorymbiahybridclones AT aloisioxavier lightqualityintheinvitrointroductionofcorymbiahybridclones AT wagnercamposotoni lightqualityintheinvitrointroductionofcorymbiahybridclones AT nataneamaralmiranda lightqualityintheinvitrointroductionofcorymbiahybridclones AT joanehelenamaggioni lightqualityintheinvitrointroductionofcorymbiahybridclones |
_version_ |
1725784600854134784 |